Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lspA and Synpcc7942_1727 are separated by 27 nucleotides Synpcc7942_1727 and Synpcc7942_1728 are separated by 152 nucleotides Synpcc7942_1728 and kdpD are separated by 5 nucleotides
Synpcc7942_1726: lspA - lipoprotein signal peptidase, at 1,799,119 to 1,799,610
lspA
Synpcc7942_1727: Synpcc7942_1727 - BioY protein, at 1,799,638 to 1,800,219
_1727
Synpcc7942_1728: Synpcc7942_1728 - hypothetical protein, at 1,800,372 to 1,800,887
_1728
Synpcc7942_1729: kdpD - potassium-transporting ATPase D chain, at 1,800,893 to 1,802,005
kdpD
Position (kb) 1800
1801 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1799.619 kb on + strand at 1799.622 kb on + strand at 1799.622 kb on - strand at 1799.630 kb on - strand at 1799.630 kb on - strand at 1800.194 kb on + strand at 1800.194 kb on + strand at 1800.229 kb on + strand at 1800.231 kb on + strand at 1800.249 kb on - strand at 1800.250 kb on + strand at 1800.250 kb on + strand at 1800.283 kb on - strand at 1800.294 kb on + strand at 1800.350 kb on + strand at 1800.358 kb on - strand at 1800.369 kb on - strand at 1800.400 kb on + strand at 1800.408 kb on - strand at 1800.418 kb on - strand at 1800.446 kb on + strand, within Synpcc7942_1728 at 1800.446 kb on + strand, within Synpcc7942_1728 at 1800.454 kb on - strand, within Synpcc7942_1728 at 1800.454 kb on - strand, within Synpcc7942_1728 at 1800.456 kb on + strand, within Synpcc7942_1728 at 1800.464 kb on - strand, within Synpcc7942_1728 at 1800.469 kb on - strand, within Synpcc7942_1728 at 1800.507 kb on + strand, within Synpcc7942_1728 at 1800.507 kb on + strand, within Synpcc7942_1728 at 1800.507 kb on + strand, within Synpcc7942_1728 at 1800.507 kb on + strand, within Synpcc7942_1728 at 1800.507 kb on + strand, within Synpcc7942_1728 at 1800.507 kb on + strand, within Synpcc7942_1728 at 1800.508 kb on - strand, within Synpcc7942_1728 at 1800.515 kb on - strand, within Synpcc7942_1728 at 1800.515 kb on - strand, within Synpcc7942_1728 at 1800.515 kb on - strand, within Synpcc7942_1728 at 1800.515 kb on - strand, within Synpcc7942_1728 at 1800.524 kb on + strand at 1800.553 kb on + strand, within Synpcc7942_1728 at 1800.568 kb on + strand, within Synpcc7942_1728 at 1800.574 kb on + strand, within Synpcc7942_1728 at 1800.574 kb on + strand, within Synpcc7942_1728 at 1800.582 kb on - strand, within Synpcc7942_1728 at 1800.582 kb on - strand, within Synpcc7942_1728 at 1800.582 kb on - strand, within Synpcc7942_1728 at 1800.582 kb on - strand, within Synpcc7942_1728 at 1800.608 kb on + strand, within Synpcc7942_1728 at 1800.621 kb on + strand, within Synpcc7942_1728 at 1800.649 kb on + strand, within Synpcc7942_1728 at 1800.649 kb on + strand, within Synpcc7942_1728 at 1800.650 kb on - strand, within Synpcc7942_1728 at 1800.666 kb on + strand, within Synpcc7942_1728 at 1800.669 kb on - strand, within Synpcc7942_1728 at 1800.674 kb on - strand, within Synpcc7942_1728 at 1800.692 kb on + strand, within Synpcc7942_1728 at 1800.695 kb on - strand, within Synpcc7942_1728 at 1800.735 kb on - strand, within Synpcc7942_1728 at 1800.735 kb on - strand, within Synpcc7942_1728 at 1800.773 kb on + strand, within Synpcc7942_1728 at 1800.773 kb on + strand, within Synpcc7942_1728 at 1800.775 kb on + strand, within Synpcc7942_1728 at 1800.781 kb on - strand, within Synpcc7942_1728 at 1800.801 kb on - strand, within Synpcc7942_1728 at 1800.819 kb on - strand, within Synpcc7942_1728 at 1800.820 kb on - strand, within Synpcc7942_1728 at 1800.820 kb on - strand, within Synpcc7942_1728 at 1800.832 kb on + strand, within Synpcc7942_1728 at 1800.850 kb on - strand at 1800.881 kb on - strand at 1800.894 kb on + strand at 1800.894 kb on + strand at 1800.898 kb on - strand at 1800.902 kb on - strand at 1800.904 kb on + strand at 1800.904 kb on + strand at 1800.904 kb on + strand at 1800.912 kb on - strand at 1800.912 kb on - strand at 1800.925 kb on + strand at 1800.948 kb on + strand at 1800.948 kb on + strand at 1800.958 kb on + strand at 1800.966 kb on - strand at 1801.002 kb on + strand at 1801.002 kb on + strand at 1801.002 kb on + strand at 1801.002 kb on + strand at 1801.002 kb on + strand at 1801.002 kb on - strand at 1801.015 kb on - strand at 1801.015 kb on - strand, within kdpD at 1801.019 kb on + strand, within kdpD at 1801.027 kb on - strand, within kdpD at 1801.113 kb on + strand, within kdpD at 1801.116 kb on - strand, within kdpD at 1801.159 kb on + strand, within kdpD at 1801.164 kb on + strand, within kdpD at 1801.167 kb on - strand, within kdpD at 1801.167 kb on - strand, within kdpD at 1801.172 kb on - strand, within kdpD at 1801.177 kb on - strand, within kdpD at 1801.187 kb on + strand, within kdpD at 1801.190 kb on - strand, within kdpD at 1801.195 kb on - strand, within kdpD at 1801.202 kb on - strand, within kdpD at 1801.213 kb on + strand, within kdpD at 1801.214 kb on - strand, within kdpD at 1801.241 kb on - strand, within kdpD at 1801.257 kb on + strand, within kdpD at 1801.257 kb on + strand, within kdpD at 1801.264 kb on - strand, within kdpD at 1801.265 kb on + strand, within kdpD at 1801.289 kb on - strand, within kdpD at 1801.316 kb on - strand, within kdpD at 1801.327 kb on - strand, within kdpD at 1801.331 kb on - strand, within kdpD at 1801.410 kb on + strand, within kdpD at 1801.410 kb on + strand, within kdpD at 1801.411 kb on - strand, within kdpD at 1801.411 kb on - strand, within kdpD at 1801.411 kb on - strand, within kdpD at 1801.457 kb on - strand, within kdpD at 1801.497 kb on + strand, within kdpD at 1801.507 kb on + strand, within kdpD at 1801.539 kb on + strand, within kdpD at 1801.547 kb on - strand, within kdpD at 1801.561 kb on + strand, within kdpD at 1801.563 kb on + strand, within kdpD at 1801.581 kb on + strand, within kdpD at 1801.581 kb on + strand, within kdpD at 1801.581 kb on + strand, within kdpD at 1801.583 kb on + strand, within kdpD at 1801.589 kb on - strand, within kdpD at 1801.589 kb on - strand, within kdpD at 1801.590 kb on + strand, within kdpD at 1801.595 kb on + strand, within kdpD at 1801.595 kb on + strand, within kdpD at 1801.600 kb on + strand, within kdpD at 1801.603 kb on - strand, within kdpD at 1801.603 kb on - strand, within kdpD at 1801.614 kb on - strand, within kdpD at 1801.654 kb on + strand, within kdpD at 1801.670 kb on - strand, within kdpD at 1801.686 kb on - strand, within kdpD at 1801.686 kb on - strand, within kdpD at 1801.722 kb on + strand, within kdpD at 1801.738 kb on - strand, within kdpD at 1801.768 kb on + strand, within kdpD at 1801.768 kb on + strand, within kdpD at 1801.796 kb on - strand, within kdpD at 1801.805 kb on - strand, within kdpD at 1801.842 kb on + strand, within kdpD at 1801.852 kb on + strand, within kdpD at 1801.856 kb on - strand, within kdpD at 1801.858 kb on - strand, within kdpD at 1801.878 kb on + strand, within kdpD
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 1,799,619 + -2.8 1,799,622 + -3.6 1,799,622 - +0.1 1,799,630 - -0.0 1,799,630 - -1.4 1,800,194 + -0.0 1,800,194 + -1.6 1,800,229 + -0.0 1,800,231 + -0.3 1,800,249 - -2.5 1,800,250 + -0.6 1,800,250 + -0.6 1,800,283 - -1.5 1,800,294 + -1.4 1,800,350 + +1.0 1,800,358 - +1.0 1,800,369 - +0.2 1,800,400 + +0.7 1,800,408 - -0.6 1,800,418 - -1.3 1,800,446 + Synpcc7942_1728 0.14 -1.3 1,800,446 + Synpcc7942_1728 0.14 +0.7 1,800,454 - Synpcc7942_1728 0.16 -1.3 1,800,454 - Synpcc7942_1728 0.16 -1.3 1,800,456 + Synpcc7942_1728 0.16 -1.3 1,800,464 - Synpcc7942_1728 0.18 -0.7 1,800,469 - Synpcc7942_1728 0.19 -0.7 1,800,507 + Synpcc7942_1728 0.26 -0.1 1,800,507 + Synpcc7942_1728 0.26 -1.8 1,800,507 + Synpcc7942_1728 0.26 -0.6 1,800,507 + Synpcc7942_1728 0.26 -0.9 1,800,507 + Synpcc7942_1728 0.26 -1.6 1,800,507 + Synpcc7942_1728 0.26 +0.3 1,800,508 - Synpcc7942_1728 0.26 -1.9 1,800,515 - Synpcc7942_1728 0.28 +1.0 1,800,515 - Synpcc7942_1728 0.28 -1.2 1,800,515 - Synpcc7942_1728 0.28 -0.9 1,800,515 - Synpcc7942_1728 0.28 -2.8 1,800,524 + +1.6 1,800,553 + Synpcc7942_1728 0.35 +1.0 1,800,568 + Synpcc7942_1728 0.38 -1.4 1,800,574 + Synpcc7942_1728 0.39 -2.2 1,800,574 + Synpcc7942_1728 0.39 -0.8 1,800,582 - Synpcc7942_1728 0.41 +1.0 1,800,582 - Synpcc7942_1728 0.41 -0.7 1,800,582 - Synpcc7942_1728 0.41 -0.4 1,800,582 - Synpcc7942_1728 0.41 -1.3 1,800,608 + Synpcc7942_1728 0.46 -1.0 1,800,621 + Synpcc7942_1728 0.48 -1.2 1,800,649 + Synpcc7942_1728 0.54 -0.0 1,800,649 + Synpcc7942_1728 0.54 -1.6 1,800,650 - Synpcc7942_1728 0.54 -1.4 1,800,666 + Synpcc7942_1728 0.57 -0.3 1,800,669 - Synpcc7942_1728 0.58 +0.1 1,800,674 - Synpcc7942_1728 0.59 -0.0 1,800,692 + Synpcc7942_1728 0.62 -1.1 1,800,695 - Synpcc7942_1728 0.63 -0.8 1,800,735 - Synpcc7942_1728 0.70 -1.0 1,800,735 - Synpcc7942_1728 0.70 -1.6 1,800,773 + Synpcc7942_1728 0.78 +1.0 1,800,773 + Synpcc7942_1728 0.78 -0.7 1,800,775 + Synpcc7942_1728 0.78 -1.3 1,800,781 - Synpcc7942_1728 0.79 -0.0 1,800,801 - Synpcc7942_1728 0.83 -0.6 1,800,819 - Synpcc7942_1728 0.87 -1.0 1,800,820 - Synpcc7942_1728 0.87 -0.6 1,800,820 - Synpcc7942_1728 0.87 -1.4 1,800,832 + Synpcc7942_1728 0.89 -1.3 1,800,850 - -0.0 1,800,881 - -0.6 1,800,894 + -0.6 1,800,894 + -1.0 1,800,898 - -1.9 1,800,902 - +1.0 1,800,904 + +1.0 1,800,904 + +0.1 1,800,904 + +1.0 1,800,912 - -0.7 1,800,912 - +0.4 1,800,925 + -0.7 1,800,948 + -1.3 1,800,948 + -0.2 1,800,958 + -0.0 1,800,966 - -0.8 1,801,002 + +0.1 1,801,002 + -1.8 1,801,002 + +1.0 1,801,002 + -0.1 1,801,002 + +1.0 1,801,002 - -0.4 1,801,015 - -1.8 1,801,015 - kdpD Synpcc7942_1729 0.11 +0.1 1,801,019 + kdpD Synpcc7942_1729 0.11 -1.8 1,801,027 - kdpD Synpcc7942_1729 0.12 -0.6 1,801,113 + kdpD Synpcc7942_1729 0.20 -0.3 1,801,116 - kdpD Synpcc7942_1729 0.20 +1.0 1,801,159 + kdpD Synpcc7942_1729 0.24 -0.8 1,801,164 + kdpD Synpcc7942_1729 0.24 +2.0 1,801,167 - kdpD Synpcc7942_1729 0.25 +0.5 1,801,167 - kdpD Synpcc7942_1729 0.25 -0.9 1,801,172 - kdpD Synpcc7942_1729 0.25 -0.0 1,801,177 - kdpD Synpcc7942_1729 0.26 -0.6 1,801,187 + kdpD Synpcc7942_1729 0.26 -0.1 1,801,190 - kdpD Synpcc7942_1729 0.27 +2.3 1,801,195 - kdpD Synpcc7942_1729 0.27 -0.1 1,801,202 - kdpD Synpcc7942_1729 0.28 -1.1 1,801,213 + kdpD Synpcc7942_1729 0.29 -0.5 1,801,214 - kdpD Synpcc7942_1729 0.29 +0.5 1,801,241 - kdpD Synpcc7942_1729 0.31 -0.9 1,801,257 + kdpD Synpcc7942_1729 0.33 -1.0 1,801,257 + kdpD Synpcc7942_1729 0.33 -1.3 1,801,264 - kdpD Synpcc7942_1729 0.33 +1.7 1,801,265 + kdpD Synpcc7942_1729 0.33 +1.0 1,801,289 - kdpD Synpcc7942_1729 0.36 -0.9 1,801,316 - kdpD Synpcc7942_1729 0.38 +1.5 1,801,327 - kdpD Synpcc7942_1729 0.39 -0.7 1,801,331 - kdpD Synpcc7942_1729 0.39 -0.9 1,801,410 + kdpD Synpcc7942_1729 0.46 -0.8 1,801,410 + kdpD Synpcc7942_1729 0.46 -1.3 1,801,411 - kdpD Synpcc7942_1729 0.47 -1.0 1,801,411 - kdpD Synpcc7942_1729 0.47 -2.0 1,801,411 - kdpD Synpcc7942_1729 0.47 -2.3 1,801,457 - kdpD Synpcc7942_1729 0.51 +2.0 1,801,497 + kdpD Synpcc7942_1729 0.54 -0.7 1,801,507 + kdpD Synpcc7942_1729 0.55 -1.4 1,801,539 + kdpD Synpcc7942_1729 0.58 -0.5 1,801,547 - kdpD Synpcc7942_1729 0.59 +0.3 1,801,561 + kdpD Synpcc7942_1729 0.60 -0.0 1,801,563 + kdpD Synpcc7942_1729 0.60 +1.1 1,801,581 + kdpD Synpcc7942_1729 0.62 -0.3 1,801,581 + kdpD Synpcc7942_1729 0.62 +1.0 1,801,581 + kdpD Synpcc7942_1729 0.62 -1.3 1,801,583 + kdpD Synpcc7942_1729 0.62 -1.3 1,801,589 - kdpD Synpcc7942_1729 0.63 +1.6 1,801,589 - kdpD Synpcc7942_1729 0.63 -0.2 1,801,590 + kdpD Synpcc7942_1729 0.63 -0.1 1,801,595 + kdpD Synpcc7942_1729 0.63 -0.6 1,801,595 + kdpD Synpcc7942_1729 0.63 -0.8 1,801,600 + kdpD Synpcc7942_1729 0.64 -0.7 1,801,603 - kdpD Synpcc7942_1729 0.64 +1.5 1,801,603 - kdpD Synpcc7942_1729 0.64 +2.8 1,801,614 - kdpD Synpcc7942_1729 0.65 -0.2 1,801,654 + kdpD Synpcc7942_1729 0.68 -0.6 1,801,670 - kdpD Synpcc7942_1729 0.70 -1.3 1,801,686 - kdpD Synpcc7942_1729 0.71 -0.5 1,801,686 - kdpD Synpcc7942_1729 0.71 -0.8 1,801,722 + kdpD Synpcc7942_1729 0.74 -0.6 1,801,738 - kdpD Synpcc7942_1729 0.76 +1.0 1,801,768 + kdpD Synpcc7942_1729 0.79 +1.0 1,801,768 + kdpD Synpcc7942_1729 0.79 -0.5 1,801,796 - kdpD Synpcc7942_1729 0.81 +1.7 1,801,805 - kdpD Synpcc7942_1729 0.82 +1.4 1,801,842 + kdpD Synpcc7942_1729 0.85 -0.6 1,801,852 + kdpD Synpcc7942_1729 0.86 -1.0 1,801,856 - kdpD Synpcc7942_1729 0.87 -0.7 1,801,858 - kdpD Synpcc7942_1729 0.87 +2.2 1,801,878 + kdpD Synpcc7942_1729 0.88 +0.2
Or see this region's nucleotide sequence