Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gldA and Synpcc7942_1654 are separated by 112 nucleotides Synpcc7942_1654 and Synpcc7942_1655 are separated by 27 nucleotides Synpcc7942_1655 and katG are separated by 89 nucleotides
Synpcc7942_1653: gldA - glycerol dehydrogenase, at 1,723,540 to 1,724,736
gldA
Synpcc7942_1654: Synpcc7942_1654 - hypothetical protein, at 1,724,849 to 1,725,232
_1654
Synpcc7942_1655: Synpcc7942_1655 - hypothetical protein, at 1,725,260 to 1,725,829
_1655
Synpcc7942_1656: katG - catalase/peroxidase HPI, at 1,725,919 to 1,728,081
katG
Position (kb) 1725
1726 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1724.276 kb on + strand, within gldA at 1724.281 kb on - strand, within gldA at 1724.283 kb on + strand, within gldA at 1724.286 kb on - strand, within gldA at 1724.291 kb on - strand, within gldA at 1724.297 kb on + strand, within gldA at 1724.297 kb on + strand, within gldA at 1724.297 kb on + strand, within gldA at 1724.300 kb on - strand, within gldA at 1724.300 kb on - strand, within gldA at 1724.300 kb on - strand, within gldA at 1724.300 kb on - strand, within gldA at 1724.305 kb on - strand, within gldA at 1724.305 kb on - strand, within gldA at 1724.350 kb on - strand, within gldA at 1724.424 kb on - strand, within gldA at 1724.443 kb on - strand, within gldA at 1724.443 kb on - strand, within gldA at 1724.462 kb on + strand, within gldA at 1724.483 kb on + strand, within gldA at 1724.491 kb on - strand, within gldA at 1724.493 kb on - strand, within gldA at 1724.496 kb on + strand, within gldA at 1724.504 kb on - strand, within gldA at 1724.515 kb on - strand, within gldA at 1724.566 kb on + strand, within gldA at 1724.607 kb on + strand, within gldA at 1724.607 kb on + strand, within gldA at 1724.615 kb on - strand, within gldA at 1724.615 kb on - strand, within gldA at 1724.615 kb on - strand, within gldA at 1724.640 kb on - strand at 1724.642 kb on - strand at 1724.664 kb on - strand at 1724.703 kb on - strand at 1724.789 kb on - strand at 1724.921 kb on + strand, within Synpcc7942_1654 at 1724.929 kb on - strand, within Synpcc7942_1654 at 1724.929 kb on - strand, within Synpcc7942_1654 at 1724.933 kb on + strand, within Synpcc7942_1654 at 1724.955 kb on - strand, within Synpcc7942_1654 at 1724.972 kb on + strand, within Synpcc7942_1654 at 1724.999 kb on + strand, within Synpcc7942_1654 at 1725.006 kb on + strand, within Synpcc7942_1654 at 1725.072 kb on - strand, within Synpcc7942_1654 at 1725.121 kb on + strand, within Synpcc7942_1654 at 1725.129 kb on - strand, within Synpcc7942_1654 at 1725.135 kb on + strand, within Synpcc7942_1654 at 1725.143 kb on - strand, within Synpcc7942_1654 at 1725.162 kb on + strand, within Synpcc7942_1654 at 1725.256 kb on - strand at 1725.279 kb on + strand at 1725.321 kb on - strand, within Synpcc7942_1655 at 1725.502 kb on + strand, within Synpcc7942_1655 at 1725.510 kb on - strand, within Synpcc7942_1655 at 1725.564 kb on - strand, within Synpcc7942_1655 at 1725.613 kb on + strand, within Synpcc7942_1655 at 1725.743 kb on + strand at 1725.803 kb on + strand at 1725.911 kb on + strand at 1725.922 kb on + strand at 1725.922 kb on + strand at 1725.946 kb on + strand at 1725.963 kb on - strand at 1725.963 kb on - strand at 1726.014 kb on + strand at 1726.014 kb on + strand at 1726.014 kb on + strand at 1726.022 kb on - strand at 1726.022 kb on - strand at 1726.022 kb on - strand at 1726.022 kb on - strand at 1726.022 kb on - strand at 1726.022 kb on - strand at 1726.022 kb on - strand at 1726.033 kb on - strand at 1726.116 kb on + strand at 1726.198 kb on + strand, within katG at 1726.199 kb on + strand, within katG at 1726.208 kb on - strand, within katG at 1726.213 kb on + strand, within katG at 1726.270 kb on + strand, within katG at 1726.279 kb on + strand, within katG at 1726.280 kb on + strand, within katG at 1726.287 kb on - strand, within katG at 1726.328 kb on + strand, within katG at 1726.336 kb on + strand, within katG at 1726.336 kb on + strand, within katG at 1726.336 kb on + strand, within katG at 1726.354 kb on + strand, within katG at 1726.383 kb on + strand, within katG at 1726.390 kb on + strand, within katG at 1726.391 kb on - strand, within katG at 1726.402 kb on + strand, within katG at 1726.412 kb on + strand, within katG at 1726.423 kb on + strand, within katG at 1726.423 kb on + strand, within katG at 1726.424 kb on + strand, within katG at 1726.431 kb on - strand, within katG at 1726.431 kb on - strand, within katG at 1726.474 kb on - strand, within katG at 1726.483 kb on - strand, within katG at 1726.483 kb on - strand, within katG at 1726.488 kb on - strand, within katG at 1726.498 kb on + strand, within katG at 1726.527 kb on + strand, within katG at 1726.591 kb on - strand, within katG at 1726.631 kb on + strand at 1726.640 kb on - strand, within katG at 1726.675 kb on - strand, within katG at 1726.676 kb on - strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.678 kb on + strand, within katG at 1726.680 kb on + strand, within katG at 1726.686 kb on - strand, within katG at 1726.686 kb on - strand, within katG at 1726.686 kb on - strand, within katG at 1726.686 kb on - strand, within katG at 1726.686 kb on - strand, within katG at 1726.686 kb on - strand, within katG at 1726.686 kb on - strand, within katG at 1726.688 kb on + strand, within katG at 1726.688 kb on + strand, within katG at 1726.688 kb on + strand, within katG at 1726.693 kb on - strand, within katG at 1726.696 kb on - strand, within katG at 1726.696 kb on - strand, within katG at 1726.701 kb on + strand, within katG at 1726.703 kb on + strand, within katG at 1726.706 kb on - strand, within katG at 1726.711 kb on - strand, within katG at 1726.711 kb on - strand at 1726.715 kb on - strand, within katG
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 1,724,276 + gldA Synpcc7942_1653 0.61 +0.4 1,724,281 - gldA Synpcc7942_1653 0.62 -0.8 1,724,283 + gldA Synpcc7942_1653 0.62 -0.7 1,724,286 - gldA Synpcc7942_1653 0.62 -0.8 1,724,291 - gldA Synpcc7942_1653 0.63 -1.2 1,724,297 + gldA Synpcc7942_1653 0.63 +1.0 1,724,297 + gldA Synpcc7942_1653 0.63 +0.3 1,724,297 + gldA Synpcc7942_1653 0.63 -1.1 1,724,300 - gldA Synpcc7942_1653 0.63 +1.0 1,724,300 - gldA Synpcc7942_1653 0.63 -0.5 1,724,300 - gldA Synpcc7942_1653 0.63 -1.1 1,724,300 - gldA Synpcc7942_1653 0.63 -0.4 1,724,305 - gldA Synpcc7942_1653 0.64 +0.0 1,724,305 - gldA Synpcc7942_1653 0.64 -1.2 1,724,350 - gldA Synpcc7942_1653 0.68 +0.4 1,724,424 - gldA Synpcc7942_1653 0.74 -0.6 1,724,443 - gldA Synpcc7942_1653 0.75 +0.7 1,724,443 - gldA Synpcc7942_1653 0.75 -1.7 1,724,462 + gldA Synpcc7942_1653 0.77 +2.6 1,724,483 + gldA Synpcc7942_1653 0.79 +0.0 1,724,491 - gldA Synpcc7942_1653 0.79 +0.4 1,724,493 - gldA Synpcc7942_1653 0.80 -1.2 1,724,496 + gldA Synpcc7942_1653 0.80 -1.8 1,724,504 - gldA Synpcc7942_1653 0.81 +0.4 1,724,515 - gldA Synpcc7942_1653 0.81 +1.0 1,724,566 + gldA Synpcc7942_1653 0.86 -1.2 1,724,607 + gldA Synpcc7942_1653 0.89 -1.4 1,724,607 + gldA Synpcc7942_1653 0.89 -0.0 1,724,615 - gldA Synpcc7942_1653 0.90 +2.6 1,724,615 - gldA Synpcc7942_1653 0.90 +0.4 1,724,615 - gldA Synpcc7942_1653 0.90 +1.6 1,724,640 - -1.6 1,724,642 - -0.8 1,724,664 - -0.4 1,724,703 - -0.6 1,724,789 - +1.6 1,724,921 + Synpcc7942_1654 0.19 -1.8 1,724,929 - Synpcc7942_1654 0.21 -1.2 1,724,929 - Synpcc7942_1654 0.21 -1.7 1,724,933 + Synpcc7942_1654 0.22 -0.6 1,724,955 - Synpcc7942_1654 0.28 -0.2 1,724,972 + Synpcc7942_1654 0.32 -0.6 1,724,999 + Synpcc7942_1654 0.39 -2.3 1,725,006 + Synpcc7942_1654 0.41 -0.7 1,725,072 - Synpcc7942_1654 0.58 -1.1 1,725,121 + Synpcc7942_1654 0.71 -0.9 1,725,129 - Synpcc7942_1654 0.73 +0.0 1,725,135 + Synpcc7942_1654 0.74 -0.3 1,725,143 - Synpcc7942_1654 0.77 -1.6 1,725,162 + Synpcc7942_1654 0.82 +0.4 1,725,256 - -1.2 1,725,279 + +1.0 1,725,321 - Synpcc7942_1655 0.11 +1.0 1,725,502 + Synpcc7942_1655 0.42 +1.0 1,725,510 - Synpcc7942_1655 0.44 -2.0 1,725,564 - Synpcc7942_1655 0.53 -1.3 1,725,613 + Synpcc7942_1655 0.62 -1.0 1,725,743 + -0.3 1,725,803 + -1.1 1,725,911 + -0.9 1,725,922 + -2.3 1,725,922 + +1.0 1,725,946 + -1.7 1,725,963 - +2.0 1,725,963 - -1.0 1,726,014 + -0.6 1,726,014 + -1.0 1,726,014 + +0.4 1,726,022 - -1.6 1,726,022 - +0.2 1,726,022 - +1.0 1,726,022 - +1.6 1,726,022 - -0.8 1,726,022 - -0.5 1,726,022 - +1.0 1,726,033 - +2.4 1,726,116 + +0.0 1,726,198 + katG Synpcc7942_1656 0.13 +1.4 1,726,199 + katG Synpcc7942_1656 0.13 +0.0 1,726,208 - katG Synpcc7942_1656 0.13 -1.7 1,726,213 + katG Synpcc7942_1656 0.14 +1.0 1,726,270 + katG Synpcc7942_1656 0.16 -1.0 1,726,279 + katG Synpcc7942_1656 0.17 +0.1 1,726,280 + katG Synpcc7942_1656 0.17 -2.3 1,726,287 - katG Synpcc7942_1656 0.17 +1.0 1,726,328 + katG Synpcc7942_1656 0.19 -0.7 1,726,336 + katG Synpcc7942_1656 0.19 +0.0 1,726,336 + katG Synpcc7942_1656 0.19 -1.5 1,726,336 + katG Synpcc7942_1656 0.19 -2.0 1,726,354 + katG Synpcc7942_1656 0.20 -1.1 1,726,383 + katG Synpcc7942_1656 0.21 +0.0 1,726,390 + katG Synpcc7942_1656 0.22 -0.2 1,726,391 - katG Synpcc7942_1656 0.22 -0.9 1,726,402 + katG Synpcc7942_1656 0.22 +1.0 1,726,412 + katG Synpcc7942_1656 0.23 -0.7 1,726,423 + katG Synpcc7942_1656 0.23 -1.6 1,726,423 + katG Synpcc7942_1656 0.23 -1.3 1,726,424 + katG Synpcc7942_1656 0.23 +0.5 1,726,431 - katG Synpcc7942_1656 0.24 -0.5 1,726,431 - katG Synpcc7942_1656 0.24 -0.1 1,726,474 - katG Synpcc7942_1656 0.26 -1.5 1,726,483 - katG Synpcc7942_1656 0.26 -1.2 1,726,483 - katG Synpcc7942_1656 0.26 -1.3 1,726,488 - katG Synpcc7942_1656 0.26 -0.7 1,726,498 + katG Synpcc7942_1656 0.27 +1.0 1,726,527 + katG Synpcc7942_1656 0.28 -0.4 1,726,591 - katG Synpcc7942_1656 0.31 -1.3 1,726,631 + -0.7 1,726,640 - katG Synpcc7942_1656 0.33 -0.3 1,726,675 - katG Synpcc7942_1656 0.35 +0.3 1,726,676 - katG Synpcc7942_1656 0.35 -0.3 1,726,678 + katG Synpcc7942_1656 0.35 -1.2 1,726,678 + katG Synpcc7942_1656 0.35 -1.3 1,726,678 + katG Synpcc7942_1656 0.35 +0.0 1,726,678 + katG Synpcc7942_1656 0.35 -0.4 1,726,678 + katG Synpcc7942_1656 0.35 -2.4 1,726,678 + katG Synpcc7942_1656 0.35 -0.4 1,726,678 + katG Synpcc7942_1656 0.35 -0.8 1,726,678 + katG Synpcc7942_1656 0.35 +0.0 1,726,678 + katG Synpcc7942_1656 0.35 +1.0 1,726,678 + katG Synpcc7942_1656 0.35 -1.8 1,726,678 + katG Synpcc7942_1656 0.35 +0.7 1,726,680 + katG Synpcc7942_1656 0.35 -0.6 1,726,686 - katG Synpcc7942_1656 0.35 -1.6 1,726,686 - katG Synpcc7942_1656 0.35 +0.0 1,726,686 - katG Synpcc7942_1656 0.35 +0.0 1,726,686 - katG Synpcc7942_1656 0.35 -1.3 1,726,686 - katG Synpcc7942_1656 0.35 -0.7 1,726,686 - katG Synpcc7942_1656 0.35 -2.9 1,726,686 - katG Synpcc7942_1656 0.35 -0.4 1,726,688 + katG Synpcc7942_1656 0.36 -1.4 1,726,688 + katG Synpcc7942_1656 0.36 +1.0 1,726,688 + katG Synpcc7942_1656 0.36 +1.0 1,726,693 - katG Synpcc7942_1656 0.36 -1.2 1,726,696 - katG Synpcc7942_1656 0.36 +0.8 1,726,696 - katG Synpcc7942_1656 0.36 +1.0 1,726,701 + katG Synpcc7942_1656 0.36 +0.4 1,726,703 + katG Synpcc7942_1656 0.36 +1.0 1,726,706 - katG Synpcc7942_1656 0.36 -0.6 1,726,711 - katG Synpcc7942_1656 0.37 -0.9 1,726,711 - -2.7 1,726,715 - katG Synpcc7942_1656 0.37 -0.7
Or see this region's nucleotide sequence