Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt somB2 and dcoHI are separated by 136 nucleotides dcoHI and psbDII are separated by 412 nucleotides
Synpcc7942_1635: somB2 - probable porin; major outer membrane protein, at 1,702,823 to 1,704,469
somB2
Synpcc7942_1636: dcoHI - pterin-4-alpha-carbinolamine dehydratase, at 1,704,606 to 1,705,013
dcoHI
Synpcc7942_1637: psbDII - photosystem II D2 protein (photosystem q(a) protein), at 1,705,426 to 1,706,484
psbDII
Position (kb)
1704
1705
1706 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1703.654 kb on + strand, within somB2 at 1703.732 kb on + strand, within somB2 at 1703.738 kb on - strand, within somB2 at 1703.740 kb on - strand, within somB2 at 1703.765 kb on + strand, within somB2 at 1703.765 kb on + strand, within somB2 at 1703.773 kb on - strand, within somB2 at 1703.773 kb on - strand, within somB2 at 1703.773 kb on - strand, within somB2 at 1703.773 kb on - strand, within somB2 at 1703.827 kb on + strand, within somB2 at 1703.827 kb on + strand, within somB2 at 1703.835 kb on - strand, within somB2 at 1703.835 kb on - strand, within somB2 at 1703.835 kb on - strand, within somB2 at 1703.844 kb on - strand, within somB2 at 1703.844 kb on - strand, within somB2 at 1703.844 kb on - strand, within somB2 at 1703.862 kb on + strand, within somB2 at 1703.870 kb on - strand, within somB2 at 1703.873 kb on + strand, within somB2 at 1703.881 kb on - strand, within somB2 at 1703.881 kb on - strand, within somB2 at 1703.881 kb on - strand, within somB2 at 1703.990 kb on - strand, within somB2 at 1704.011 kb on - strand, within somB2 at 1704.300 kb on - strand, within somB2 at 1704.302 kb on + strand, within somB2 at 1704.322 kb on - strand at 1704.337 kb on - strand at 1704.367 kb on + strand at 1704.561 kb on + strand at 1704.565 kb on + strand at 1704.579 kb on - strand at 1704.612 kb on - strand at 1704.612 kb on - strand at 1704.614 kb on + strand at 1704.641 kb on - strand at 1704.648 kb on + strand, within dcoHI at 1704.649 kb on + strand, within dcoHI at 1704.696 kb on + strand at 1704.701 kb on - strand, within dcoHI at 1704.704 kb on - strand, within dcoHI at 1704.769 kb on - strand, within dcoHI at 1704.770 kb on + strand, within dcoHI at 1704.840 kb on + strand, within dcoHI at 1704.865 kb on + strand, within dcoHI at 1704.873 kb on - strand, within dcoHI at 1704.875 kb on - strand at 1704.875 kb on - strand, within dcoHI at 1704.877 kb on + strand, within dcoHI at 1704.877 kb on + strand, within dcoHI at 1704.880 kb on - strand, within dcoHI at 1704.932 kb on - strand, within dcoHI at 1704.933 kb on + strand, within dcoHI at 1704.933 kb on - strand, within dcoHI at 1704.940 kb on + strand, within dcoHI at 1704.944 kb on + strand, within dcoHI at 1704.945 kb on + strand, within dcoHI at 1704.948 kb on - strand, within dcoHI at 1704.948 kb on - strand, within dcoHI at 1704.948 kb on - strand, within dcoHI at 1704.972 kb on + strand, within dcoHI at 1704.978 kb on + strand at 1704.980 kb on - strand at 1704.994 kb on + strand at 1705.002 kb on - strand at 1705.002 kb on - strand at 1705.004 kb on + strand at 1705.012 kb on - strand at 1705.017 kb on + strand at 1705.020 kb on - strand at 1705.027 kb on + strand at 1705.027 kb on - strand at 1705.098 kb on + strand at 1705.106 kb on - strand at 1705.106 kb on - strand at 1705.127 kb on + strand at 1705.166 kb on - strand at 1705.213 kb on - strand at 1705.270 kb on + strand at 1705.282 kb on + strand at 1705.295 kb on + strand at 1705.326 kb on - strand at 1705.326 kb on - strand at 1705.344 kb on + strand at 1705.352 kb on - strand at 1705.352 kb on - strand at 1705.377 kb on + strand at 1705.377 kb on + strand at 1705.377 kb on + strand at 1705.377 kb on + strand at 1705.385 kb on - strand at 1705.385 kb on - strand at 1705.433 kb on + strand at 1705.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 1,703,654 + somB2 Synpcc7942_1635 0.50 +0.0 1,703,732 + somB2 Synpcc7942_1635 0.55 +1.0 1,703,738 - somB2 Synpcc7942_1635 0.56 +0.8 1,703,740 - somB2 Synpcc7942_1635 0.56 +1.5 1,703,765 + somB2 Synpcc7942_1635 0.57 +1.2 1,703,765 + somB2 Synpcc7942_1635 0.57 -0.6 1,703,773 - somB2 Synpcc7942_1635 0.58 +1.6 1,703,773 - somB2 Synpcc7942_1635 0.58 +1.8 1,703,773 - somB2 Synpcc7942_1635 0.58 +1.5 1,703,773 - somB2 Synpcc7942_1635 0.58 +3.0 1,703,827 + somB2 Synpcc7942_1635 0.61 -0.7 1,703,827 + somB2 Synpcc7942_1635 0.61 +0.0 1,703,835 - somB2 Synpcc7942_1635 0.61 +1.7 1,703,835 - somB2 Synpcc7942_1635 0.61 +0.9 1,703,835 - somB2 Synpcc7942_1635 0.61 +0.8 1,703,844 - somB2 Synpcc7942_1635 0.62 +1.5 1,703,844 - somB2 Synpcc7942_1635 0.62 +0.6 1,703,844 - somB2 Synpcc7942_1635 0.62 +0.9 1,703,862 + somB2 Synpcc7942_1635 0.63 +2.0 1,703,870 - somB2 Synpcc7942_1635 0.64 +1.3 1,703,873 + somB2 Synpcc7942_1635 0.64 +0.4 1,703,881 - somB2 Synpcc7942_1635 0.64 +1.4 1,703,881 - somB2 Synpcc7942_1635 0.64 +2.0 1,703,881 - somB2 Synpcc7942_1635 0.64 +1.8 1,703,990 - somB2 Synpcc7942_1635 0.71 +1.4 1,704,011 - somB2 Synpcc7942_1635 0.72 +0.7 1,704,300 - somB2 Synpcc7942_1635 0.90 +1.5 1,704,302 + somB2 Synpcc7942_1635 0.90 +1.0 1,704,322 - +0.1 1,704,337 - -0.3 1,704,367 + +0.0 1,704,561 + -1.1 1,704,565 + +2.0 1,704,579 - -0.6 1,704,612 - -0.4 1,704,612 - -0.4 1,704,614 + +0.2 1,704,641 - -1.1 1,704,648 + dcoHI Synpcc7942_1636 0.10 -0.1 1,704,649 + dcoHI Synpcc7942_1636 0.11 -0.7 1,704,696 + -0.9 1,704,701 - dcoHI Synpcc7942_1636 0.23 +2.5 1,704,704 - dcoHI Synpcc7942_1636 0.24 +1.0 1,704,769 - dcoHI Synpcc7942_1636 0.40 +0.6 1,704,770 + dcoHI Synpcc7942_1636 0.40 -1.2 1,704,840 + dcoHI Synpcc7942_1636 0.57 +0.4 1,704,865 + dcoHI Synpcc7942_1636 0.63 +0.5 1,704,873 - dcoHI Synpcc7942_1636 0.65 +0.6 1,704,875 - +0.6 1,704,875 - dcoHI Synpcc7942_1636 0.66 -1.2 1,704,877 + dcoHI Synpcc7942_1636 0.66 -0.5 1,704,877 + dcoHI Synpcc7942_1636 0.66 +3.8 1,704,880 - dcoHI Synpcc7942_1636 0.67 -0.6 1,704,932 - dcoHI Synpcc7942_1636 0.80 -0.5 1,704,933 + dcoHI Synpcc7942_1636 0.80 -0.6 1,704,933 - dcoHI Synpcc7942_1636 0.80 -0.6 1,704,940 + dcoHI Synpcc7942_1636 0.82 +0.0 1,704,944 + dcoHI Synpcc7942_1636 0.83 +0.0 1,704,945 + dcoHI Synpcc7942_1636 0.83 -0.6 1,704,948 - dcoHI Synpcc7942_1636 0.84 +0.6 1,704,948 - dcoHI Synpcc7942_1636 0.84 -0.5 1,704,948 - dcoHI Synpcc7942_1636 0.84 +2.0 1,704,972 + dcoHI Synpcc7942_1636 0.90 -1.0 1,704,978 + -0.6 1,704,980 - -1.1 1,704,994 + -0.9 1,705,002 - +0.6 1,705,002 - -1.3 1,705,004 + -0.1 1,705,012 - -1.7 1,705,017 + -0.9 1,705,020 - +2.0 1,705,027 + +2.2 1,705,027 - -1.7 1,705,098 + +2.0 1,705,106 - +1.6 1,705,106 - -1.3 1,705,127 + -0.3 1,705,166 - +0.3 1,705,213 - -0.5 1,705,270 + -0.6 1,705,282 + +0.7 1,705,295 + -0.8 1,705,326 - +0.0 1,705,326 - -0.5 1,705,344 + -0.2 1,705,352 - +1.3 1,705,352 - +2.3 1,705,377 + -0.4 1,705,377 + +0.0 1,705,377 + -0.2 1,705,377 + -0.9 1,705,385 - +2.0 1,705,385 - +1.0 1,705,433 + -0.1 1,705,441 - +1.0
Or see this region's nucleotide sequence