Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysM and Synpcc7942_1467 are separated by 5 nucleotides Synpcc7942_1467 and Synpcc7942_1468 are separated by 118 nucleotides Synpcc7942_1468 and Synpcc7942_1469 overlap by 4 nucleotides
Synpcc7942_1466: cysM - cysteine synthase, at 1,519,664 to 1,520,668
cysM
Synpcc7942_1467: Synpcc7942_1467 - Heat shock protein DnaJ-like, at 1,520,674 to 1,521,363
_1467
Synpcc7942_1468: Synpcc7942_1468 - putative monovalent cation/H+ antiporter subunit B, at 1,521,482 to 1,522,141
_1468
Synpcc7942_1469: Synpcc7942_1469 - hypothetical protein, at 1,522,138 to 1,522,719
_1469
Position (kb)
1520
1521
1522 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1519.681 kb on + strand at 1519.689 kb on - strand at 1519.689 kb on - strand at 1519.696 kb on + strand at 1519.698 kb on + strand at 1519.711 kb on - strand at 1519.711 kb on - strand at 1519.725 kb on + strand at 1519.733 kb on - strand at 1519.756 kb on - strand at 1519.756 kb on - strand at 1519.757 kb on - strand at 1519.759 kb on + strand at 1519.860 kb on + strand, within cysM at 1519.887 kb on - strand, within cysM at 1519.919 kb on - strand, within cysM at 1519.984 kb on + strand, within cysM at 1520.067 kb on + strand, within cysM at 1520.088 kb on - strand, within cysM at 1520.093 kb on - strand, within cysM at 1520.146 kb on + strand, within cysM at 1520.187 kb on + strand, within cysM at 1520.250 kb on - strand, within cysM at 1520.268 kb on + strand, within cysM at 1520.301 kb on + strand at 1520.324 kb on - strand, within cysM at 1520.380 kb on - strand, within cysM at 1520.412 kb on + strand, within cysM at 1520.439 kb on - strand, within cysM at 1520.500 kb on - strand, within cysM at 1520.595 kb on + strand at 1520.595 kb on + strand at 1520.595 kb on + strand at 1520.608 kb on - strand at 1520.621 kb on + strand at 1520.691 kb on + strand at 1520.763 kb on - strand, within Synpcc7942_1467 at 1520.827 kb on + strand, within Synpcc7942_1467 at 1520.858 kb on + strand, within Synpcc7942_1467 at 1520.858 kb on + strand, within Synpcc7942_1467 at 1520.863 kb on + strand, within Synpcc7942_1467 at 1520.895 kb on + strand, within Synpcc7942_1467 at 1520.897 kb on + strand, within Synpcc7942_1467 at 1520.911 kb on + strand, within Synpcc7942_1467 at 1520.971 kb on - strand, within Synpcc7942_1467 at 1520.971 kb on - strand, within Synpcc7942_1467 at 1520.993 kb on - strand, within Synpcc7942_1467 at 1521.006 kb on - strand, within Synpcc7942_1467 at 1521.038 kb on - strand, within Synpcc7942_1467 at 1521.048 kb on - strand, within Synpcc7942_1467 at 1521.066 kb on - strand, within Synpcc7942_1467 at 1521.069 kb on - strand, within Synpcc7942_1467 at 1521.096 kb on + strand, within Synpcc7942_1467 at 1521.166 kb on + strand, within Synpcc7942_1467 at 1521.213 kb on + strand, within Synpcc7942_1467 at 1521.374 kb on - strand at 1521.376 kb on + strand at 1521.399 kb on + strand at 1521.480 kb on + strand at 1521.584 kb on + strand, within Synpcc7942_1468 at 1521.599 kb on + strand, within Synpcc7942_1468 at 1521.614 kb on - strand, within Synpcc7942_1468 at 1521.795 kb on - strand, within Synpcc7942_1468 at 1522.284 kb on + strand, within Synpcc7942_1469 at 1522.332 kb on - strand, within Synpcc7942_1469 at 1522.332 kb on - strand, within Synpcc7942_1469
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 1,519,681 + -0.8 1,519,689 - +2.0 1,519,689 - +0.8 1,519,696 + +1.4 1,519,698 + -0.7 1,519,711 - -1.7 1,519,711 - -1.7 1,519,725 + +0.4 1,519,733 - -0.9 1,519,756 - -1.0 1,519,756 - +0.0 1,519,757 - +1.0 1,519,759 + +0.0 1,519,860 + cysM Synpcc7942_1466 0.20 +2.0 1,519,887 - cysM Synpcc7942_1466 0.22 -1.0 1,519,919 - cysM Synpcc7942_1466 0.25 -0.6 1,519,984 + cysM Synpcc7942_1466 0.32 +0.0 1,520,067 + cysM Synpcc7942_1466 0.40 -0.7 1,520,088 - cysM Synpcc7942_1466 0.42 +1.0 1,520,093 - cysM Synpcc7942_1466 0.43 -0.7 1,520,146 + cysM Synpcc7942_1466 0.48 +2.0 1,520,187 + cysM Synpcc7942_1466 0.52 -0.5 1,520,250 - cysM Synpcc7942_1466 0.58 -1.5 1,520,268 + cysM Synpcc7942_1466 0.60 +1.0 1,520,301 + +0.0 1,520,324 - cysM Synpcc7942_1466 0.66 -2.3 1,520,380 - cysM Synpcc7942_1466 0.71 -2.3 1,520,412 + cysM Synpcc7942_1466 0.74 -1.0 1,520,439 - cysM Synpcc7942_1466 0.77 -0.7 1,520,500 - cysM Synpcc7942_1466 0.83 +0.4 1,520,595 + -1.5 1,520,595 + -0.6 1,520,595 + +0.3 1,520,608 - +0.4 1,520,621 + +0.0 1,520,691 + -1.7 1,520,763 - Synpcc7942_1467 0.13 -1.0 1,520,827 + Synpcc7942_1467 0.22 -0.4 1,520,858 + Synpcc7942_1467 0.27 +1.0 1,520,858 + Synpcc7942_1467 0.27 -0.9 1,520,863 + Synpcc7942_1467 0.27 +1.4 1,520,895 + Synpcc7942_1467 0.32 +0.8 1,520,897 + Synpcc7942_1467 0.32 -1.8 1,520,911 + Synpcc7942_1467 0.34 -1.5 1,520,971 - Synpcc7942_1467 0.43 -0.9 1,520,971 - Synpcc7942_1467 0.43 +0.3 1,520,993 - Synpcc7942_1467 0.46 -0.6 1,521,006 - Synpcc7942_1467 0.48 +0.3 1,521,038 - Synpcc7942_1467 0.53 -1.5 1,521,048 - Synpcc7942_1467 0.54 -1.9 1,521,066 - Synpcc7942_1467 0.57 -1.3 1,521,069 - Synpcc7942_1467 0.57 +1.0 1,521,096 + Synpcc7942_1467 0.61 -0.3 1,521,166 + Synpcc7942_1467 0.71 +0.0 1,521,213 + Synpcc7942_1467 0.78 -1.0 1,521,374 - +0.2 1,521,376 + +1.0 1,521,399 + -0.8 1,521,480 + +1.0 1,521,584 + Synpcc7942_1468 0.15 +1.0 1,521,599 + Synpcc7942_1468 0.18 +1.0 1,521,614 - Synpcc7942_1468 0.20 +1.0 1,521,795 - Synpcc7942_1468 0.47 +1.0 1,522,284 + Synpcc7942_1469 0.25 +0.0 1,522,332 - Synpcc7942_1469 0.33 +1.0 1,522,332 - Synpcc7942_1469 0.33 +1.0
Or see this region's nucleotide sequence