Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pmbA and smtA are separated by 108 nucleotides smtA and smtB are separated by 100 nucleotides smtB and dnaG are separated by 9 nucleotides dnaG and pheT are separated by 142 nucleotides
Synpcc7942_1289: pmbA - putative modulator of DNA gyrase, at 1,311,841 to 1,313,181
pmbA
Synpcc7942_1290: smtA - SmtA metallothionein, at 1,313,290 to 1,313,460
smtA
Synpcc7942_1291: smtB - transcriptional regulator, ArsR family, at 1,313,561 to 1,313,929
smtB
Synpcc7942_1292: dnaG - DNA primase, at 1,313,939 to 1,316,023
dnaG
Synpcc7942_1293: pheT - phenylalanyl-tRNA synthetase subunit beta, at 1,316,166 to 1,318,598
pheT
Position (kb)
1313
1314
1315
1316
1317 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1312.962 kb on + strand, within pmbA at 1312.970 kb on - strand, within pmbA at 1313.080 kb on + strand at 1313.085 kb on + strand at 1313.118 kb on + strand at 1313.126 kb on - strand at 1313.151 kb on + strand at 1313.182 kb on + strand at 1313.182 kb on + strand at 1313.184 kb on + strand at 1313.185 kb on + strand at 1313.185 kb on + strand at 1313.193 kb on - strand at 1313.193 kb on - strand at 1313.193 kb on - strand at 1313.193 kb on - strand at 1313.198 kb on + strand at 1313.206 kb on - strand at 1313.230 kb on + strand at 1313.252 kb on - strand at 1313.291 kb on - strand at 1313.356 kb on + strand, within smtA at 1313.366 kb on + strand, within smtA at 1313.370 kb on + strand, within smtA at 1313.371 kb on + strand, within smtA at 1313.416 kb on - strand at 1313.424 kb on + strand, within smtA at 1313.440 kb on + strand, within smtA at 1313.455 kb on + strand at 1313.457 kb on - strand at 1313.563 kb on + strand at 1313.586 kb on + strand at 1313.589 kb on - strand at 1313.594 kb on + strand at 1313.625 kb on + strand, within smtB at 1313.665 kb on - strand, within smtB at 1313.684 kb on + strand, within smtB at 1313.692 kb on - strand, within smtB at 1313.716 kb on + strand, within smtB at 1313.716 kb on + strand, within smtB at 1313.716 kb on + strand, within smtB at 1313.716 kb on + strand, within smtB at 1313.737 kb on + strand, within smtB at 1313.740 kb on + strand, within smtB at 1313.750 kb on - strand, within smtB at 1313.761 kb on + strand, within smtB at 1313.763 kb on + strand, within smtB at 1313.814 kb on + strand, within smtB at 1313.852 kb on + strand, within smtB at 1313.852 kb on + strand, within smtB at 1313.857 kb on + strand, within smtB at 1313.860 kb on - strand, within smtB at 1313.878 kb on - strand, within smtB at 1313.936 kb on + strand at 1313.937 kb on + strand at 1313.977 kb on + strand at 1313.977 kb on + strand at 1313.979 kb on + strand at 1313.984 kb on + strand at 1313.985 kb on - strand at 1314.002 kb on - strand at 1314.007 kb on - strand at 1316.029 kb on + strand at 1316.046 kb on - strand at 1316.069 kb on - strand at 1316.071 kb on + strand at 1316.079 kb on - strand at 1316.138 kb on + strand at 1316.139 kb on - strand at 1316.153 kb on + strand at 1316.161 kb on + strand at 1316.161 kb on - strand at 1316.161 kb on - strand at 1316.163 kb on + strand at 1316.163 kb on + strand at 1316.170 kb on - strand at 1316.170 kb on - strand at 1316.170 kb on - strand at 1316.170 kb on - strand at 1316.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 1,312,962 + pmbA Synpcc7942_1289 0.84 -0.6 1,312,970 - pmbA Synpcc7942_1289 0.84 +0.0 1,313,080 + +2.0 1,313,085 + +0.0 1,313,118 + -0.8 1,313,126 - +1.0 1,313,151 + +0.0 1,313,182 + +1.0 1,313,182 + -0.7 1,313,184 + +1.0 1,313,185 + -1.2 1,313,185 + -0.2 1,313,193 - +0.8 1,313,193 - +1.0 1,313,193 - -1.0 1,313,193 - +1.5 1,313,198 + +0.6 1,313,206 - +0.3 1,313,230 + -1.1 1,313,252 - -1.3 1,313,291 - +1.0 1,313,356 + smtA Synpcc7942_1290 0.39 -2.0 1,313,366 + smtA Synpcc7942_1290 0.44 +1.0 1,313,370 + smtA Synpcc7942_1290 0.47 +1.0 1,313,371 + smtA Synpcc7942_1290 0.47 -2.8 1,313,416 - -0.2 1,313,424 + smtA Synpcc7942_1290 0.78 -2.6 1,313,440 + smtA Synpcc7942_1290 0.88 +1.0 1,313,455 + +1.0 1,313,457 - +1.0 1,313,563 + +0.0 1,313,586 + -0.6 1,313,589 - +1.0 1,313,594 + +0.2 1,313,625 + smtB Synpcc7942_1291 0.17 +0.5 1,313,665 - smtB Synpcc7942_1291 0.28 +0.0 1,313,684 + smtB Synpcc7942_1291 0.33 +0.0 1,313,692 - smtB Synpcc7942_1291 0.36 +1.4 1,313,716 + smtB Synpcc7942_1291 0.42 -0.9 1,313,716 + smtB Synpcc7942_1291 0.42 +1.0 1,313,716 + smtB Synpcc7942_1291 0.42 -0.3 1,313,716 + smtB Synpcc7942_1291 0.42 +0.0 1,313,737 + smtB Synpcc7942_1291 0.48 -0.3 1,313,740 + smtB Synpcc7942_1291 0.49 +0.0 1,313,750 - smtB Synpcc7942_1291 0.51 -0.1 1,313,761 + smtB Synpcc7942_1291 0.54 +0.4 1,313,763 + smtB Synpcc7942_1291 0.55 +1.0 1,313,814 + smtB Synpcc7942_1291 0.69 +1.0 1,313,852 + smtB Synpcc7942_1291 0.79 +0.0 1,313,852 + smtB Synpcc7942_1291 0.79 +2.6 1,313,857 + smtB Synpcc7942_1291 0.80 -0.3 1,313,860 - smtB Synpcc7942_1291 0.81 -0.6 1,313,878 - smtB Synpcc7942_1291 0.86 -0.3 1,313,936 + +1.0 1,313,937 + +1.0 1,313,977 + +1.0 1,313,977 + -0.6 1,313,979 + -1.2 1,313,984 + -1.6 1,313,985 - +1.5 1,314,002 - +0.6 1,314,007 - -0.7 1,316,029 + +0.4 1,316,046 - +1.0 1,316,069 - -0.3 1,316,071 + -2.0 1,316,079 - +0.4 1,316,138 + +2.0 1,316,139 - +0.6 1,316,153 + -0.3 1,316,161 + +3.0 1,316,161 - +1.0 1,316,161 - -1.1 1,316,163 + +0.0 1,316,163 + +0.1 1,316,170 - -1.1 1,316,170 - -0.4 1,316,170 - -0.1 1,316,170 - +3.4 1,316,171 - +2.0
Or see this region's nucleotide sequence