Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pgk and Synpcc7942_1117 are separated by 204 nucleotides Synpcc7942_1117 and Synpcc7942_1118 are separated by 20 nucleotides Synpcc7942_1118 and Synpcc7942_1119 are separated by 2 nucleotides
Synpcc7942_1116: pgk - phosphoglycerate kinase, at 1,132,968 to 1,134,176
pgk
Synpcc7942_1117: Synpcc7942_1117 - hypothetical protein, at 1,134,381 to 1,135,043
_1117
Synpcc7942_1118: Synpcc7942_1118 - mercuric reductase, at 1,135,064 to 1,136,614
_1118
Synpcc7942_1119: Synpcc7942_1119 - hypothetical protein, at 1,136,617 to 1,137,294
_1119
Position (kb)
1135
1136
1137 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1134.171 kb on + strand at 1134.171 kb on + strand at 1134.171 kb on + strand at 1134.171 kb on + strand at 1134.179 kb on - strand at 1134.179 kb on - strand at 1134.179 kb on - strand at 1134.257 kb on + strand at 1134.274 kb on + strand at 1134.274 kb on + strand at 1134.282 kb on - strand at 1134.283 kb on - strand at 1134.310 kb on - strand at 1134.405 kb on + strand at 1134.468 kb on + strand, within Synpcc7942_1117 at 1134.549 kb on - strand, within Synpcc7942_1117 at 1134.564 kb on + strand, within Synpcc7942_1117 at 1134.583 kb on + strand, within Synpcc7942_1117 at 1134.590 kb on - strand, within Synpcc7942_1117 at 1134.594 kb on + strand, within Synpcc7942_1117 at 1134.677 kb on + strand, within Synpcc7942_1117 at 1134.756 kb on - strand, within Synpcc7942_1117 at 1134.815 kb on + strand, within Synpcc7942_1117 at 1134.825 kb on - strand, within Synpcc7942_1117 at 1134.829 kb on + strand, within Synpcc7942_1117 at 1134.829 kb on + strand, within Synpcc7942_1117 at 1134.837 kb on - strand, within Synpcc7942_1117 at 1134.837 kb on - strand, within Synpcc7942_1117 at 1134.837 kb on - strand, within Synpcc7942_1117 at 1134.837 kb on - strand, within Synpcc7942_1117 at 1134.948 kb on + strand, within Synpcc7942_1117 at 1135.130 kb on - strand at 1135.188 kb on + strand at 1135.337 kb on + strand, within Synpcc7942_1118 at 1135.345 kb on - strand, within Synpcc7942_1118 at 1135.345 kb on - strand, within Synpcc7942_1118 at 1135.428 kb on + strand, within Synpcc7942_1118 at 1135.542 kb on + strand, within Synpcc7942_1118 at 1135.588 kb on - strand, within Synpcc7942_1118 at 1135.833 kb on - strand, within Synpcc7942_1118 at 1135.869 kb on - strand, within Synpcc7942_1118 at 1135.926 kb on - strand, within Synpcc7942_1118 at 1135.928 kb on + strand, within Synpcc7942_1118 at 1135.974 kb on + strand, within Synpcc7942_1118 at 1136.001 kb on + strand, within Synpcc7942_1118 at 1136.001 kb on + strand, within Synpcc7942_1118 at 1136.009 kb on - strand, within Synpcc7942_1118 at 1136.020 kb on - strand, within Synpcc7942_1118 at 1136.198 kb on - strand, within Synpcc7942_1118 at 1136.203 kb on + strand, within Synpcc7942_1118 at 1136.224 kb on + strand, within Synpcc7942_1118 at 1136.249 kb on + strand, within Synpcc7942_1118 at 1136.249 kb on + strand, within Synpcc7942_1118 at 1136.257 kb on - strand, within Synpcc7942_1118 at 1136.257 kb on - strand, within Synpcc7942_1118 at 1136.257 kb on - strand, within Synpcc7942_1118 at 1136.257 kb on - strand, within Synpcc7942_1118 at 1136.293 kb on + strand, within Synpcc7942_1118 at 1136.309 kb on + strand, within Synpcc7942_1118 at 1136.326 kb on + strand, within Synpcc7942_1118 at 1136.356 kb on + strand, within Synpcc7942_1118 at 1136.435 kb on + strand, within Synpcc7942_1118 at 1136.435 kb on + strand, within Synpcc7942_1118 at 1136.435 kb on + strand, within Synpcc7942_1118 at 1136.435 kb on + strand, within Synpcc7942_1118 at 1136.435 kb on + strand, within Synpcc7942_1118 at 1136.443 kb on - strand, within Synpcc7942_1118 at 1136.443 kb on - strand, within Synpcc7942_1118 at 1136.443 kb on - strand, within Synpcc7942_1118 at 1136.443 kb on - strand, within Synpcc7942_1118 at 1136.443 kb on - strand, within Synpcc7942_1118 at 1136.443 kb on - strand, within Synpcc7942_1118 at 1136.457 kb on - strand, within Synpcc7942_1118 at 1136.514 kb on + strand at 1136.517 kb on - strand at 1136.531 kb on + strand at 1136.534 kb on - strand at 1136.583 kb on - strand at 1136.603 kb on - strand at 1136.617 kb on + strand at 1136.627 kb on + strand at 1136.643 kb on - strand at 1136.666 kb on - strand at 1136.728 kb on + strand, within Synpcc7942_1119 at 1136.850 kb on - strand, within Synpcc7942_1119 at 1136.864 kb on - strand, within Synpcc7942_1119 at 1136.871 kb on - strand, within Synpcc7942_1119 at 1136.881 kb on - strand, within Synpcc7942_1119 at 1136.915 kb on - strand, within Synpcc7942_1119 at 1136.917 kb on - strand, within Synpcc7942_1119 at 1136.945 kb on + strand, within Synpcc7942_1119 at 1136.953 kb on - strand, within Synpcc7942_1119 at 1136.960 kb on - strand, within Synpcc7942_1119 at 1137.005 kb on + strand, within Synpcc7942_1119 at 1137.013 kb on - strand, within Synpcc7942_1119 at 1137.013 kb on - strand, within Synpcc7942_1119 at 1137.059 kb on - strand, within Synpcc7942_1119 at 1137.059 kb on - strand, within Synpcc7942_1119 at 1137.071 kb on + strand, within Synpcc7942_1119 at 1137.073 kb on + strand, within Synpcc7942_1119 at 1137.129 kb on - strand, within Synpcc7942_1119 at 1137.204 kb on + strand, within Synpcc7942_1119 at 1137.214 kb on + strand, within Synpcc7942_1119 at 1137.264 kb on - strand at 1137.278 kb on + strand at 1137.283 kb on + strand at 1137.299 kb on - strand at 1137.299 kb on - strand at 1137.313 kb on - strand at 1137.328 kb on + strand at 1137.336 kb on - strand at 1137.343 kb on - strand at 1137.383 kb on + strand at 1137.383 kb on + strand at 1137.384 kb on - strand at 1137.391 kb on - strand at 1137.391 kb on - strand at 1137.464 kb on + strand at 1137.464 kb on + strand at 1137.464 kb on + strand at 1137.472 kb on - strand at 1137.499 kb on + strand at 1137.519 kb on - strand at 1137.519 kb on - strand at 1137.519 kb on - strand at 1137.547 kb on + strand at 1137.571 kb on + strand at 1137.599 kb on + strand at 1137.614 kb on - strand at 1137.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 1,134,171 + +0.0 1,134,171 + +1.0 1,134,171 + +2.0 1,134,171 + -0.6 1,134,179 - -0.6 1,134,179 - +0.0 1,134,179 - -0.1 1,134,257 + -0.7 1,134,274 + -1.3 1,134,274 + -1.1 1,134,282 - -0.7 1,134,283 - -1.3 1,134,310 - +1.0 1,134,405 + -0.4 1,134,468 + Synpcc7942_1117 0.13 -1.0 1,134,549 - Synpcc7942_1117 0.25 -0.3 1,134,564 + Synpcc7942_1117 0.28 -0.3 1,134,583 + Synpcc7942_1117 0.30 +1.5 1,134,590 - Synpcc7942_1117 0.32 -0.7 1,134,594 + Synpcc7942_1117 0.32 +0.3 1,134,677 + Synpcc7942_1117 0.45 -0.8 1,134,756 - Synpcc7942_1117 0.57 +0.2 1,134,815 + Synpcc7942_1117 0.65 +0.2 1,134,825 - Synpcc7942_1117 0.67 +0.6 1,134,829 + Synpcc7942_1117 0.68 +1.3 1,134,829 + Synpcc7942_1117 0.68 -0.2 1,134,837 - Synpcc7942_1117 0.69 +1.8 1,134,837 - Synpcc7942_1117 0.69 -1.8 1,134,837 - Synpcc7942_1117 0.69 +1.0 1,134,837 - Synpcc7942_1117 0.69 -1.3 1,134,948 + Synpcc7942_1117 0.86 -0.1 1,135,130 - +0.5 1,135,188 + +1.2 1,135,337 + Synpcc7942_1118 0.18 -1.1 1,135,345 - Synpcc7942_1118 0.18 -0.7 1,135,345 - Synpcc7942_1118 0.18 -1.0 1,135,428 + Synpcc7942_1118 0.23 +0.0 1,135,542 + Synpcc7942_1118 0.31 +0.0 1,135,588 - Synpcc7942_1118 0.34 +1.0 1,135,833 - Synpcc7942_1118 0.50 +1.6 1,135,869 - Synpcc7942_1118 0.52 -1.7 1,135,926 - Synpcc7942_1118 0.56 -1.0 1,135,928 + Synpcc7942_1118 0.56 -0.2 1,135,974 + Synpcc7942_1118 0.59 +0.7 1,136,001 + Synpcc7942_1118 0.60 +1.0 1,136,001 + Synpcc7942_1118 0.60 -1.3 1,136,009 - Synpcc7942_1118 0.61 -1.3 1,136,020 - Synpcc7942_1118 0.62 -3.8 1,136,198 - Synpcc7942_1118 0.73 -2.0 1,136,203 + Synpcc7942_1118 0.73 -1.6 1,136,224 + Synpcc7942_1118 0.75 -0.5 1,136,249 + Synpcc7942_1118 0.76 -1.8 1,136,249 + Synpcc7942_1118 0.76 +1.0 1,136,257 - Synpcc7942_1118 0.77 -0.8 1,136,257 - Synpcc7942_1118 0.77 -0.9 1,136,257 - Synpcc7942_1118 0.77 -0.5 1,136,257 - Synpcc7942_1118 0.77 -2.4 1,136,293 + Synpcc7942_1118 0.79 -0.5 1,136,309 + Synpcc7942_1118 0.80 -0.1 1,136,326 + Synpcc7942_1118 0.81 +0.0 1,136,356 + Synpcc7942_1118 0.83 -1.8 1,136,435 + Synpcc7942_1118 0.88 +1.0 1,136,435 + Synpcc7942_1118 0.88 -0.6 1,136,435 + Synpcc7942_1118 0.88 -1.0 1,136,435 + Synpcc7942_1118 0.88 +1.6 1,136,435 + Synpcc7942_1118 0.88 +0.7 1,136,443 - Synpcc7942_1118 0.89 +0.3 1,136,443 - Synpcc7942_1118 0.89 -0.7 1,136,443 - Synpcc7942_1118 0.89 -0.5 1,136,443 - Synpcc7942_1118 0.89 -1.4 1,136,443 - Synpcc7942_1118 0.89 -1.1 1,136,443 - Synpcc7942_1118 0.89 +0.0 1,136,457 - Synpcc7942_1118 0.90 -1.8 1,136,514 + +0.4 1,136,517 - +1.0 1,136,531 + -0.4 1,136,534 - -0.9 1,136,583 - -1.1 1,136,603 - +1.0 1,136,617 + +0.5 1,136,627 + +1.0 1,136,643 - -0.8 1,136,666 - -0.9 1,136,728 + Synpcc7942_1119 0.16 -0.6 1,136,850 - Synpcc7942_1119 0.34 +0.0 1,136,864 - Synpcc7942_1119 0.36 +1.0 1,136,871 - Synpcc7942_1119 0.37 -0.6 1,136,881 - Synpcc7942_1119 0.39 -1.0 1,136,915 - Synpcc7942_1119 0.44 -0.8 1,136,917 - Synpcc7942_1119 0.44 -1.4 1,136,945 + Synpcc7942_1119 0.48 +0.9 1,136,953 - Synpcc7942_1119 0.50 -0.6 1,136,960 - Synpcc7942_1119 0.51 +1.6 1,137,005 + Synpcc7942_1119 0.57 -0.4 1,137,013 - Synpcc7942_1119 0.58 -1.2 1,137,013 - Synpcc7942_1119 0.58 -1.9 1,137,059 - Synpcc7942_1119 0.65 -1.0 1,137,059 - Synpcc7942_1119 0.65 +0.0 1,137,071 + Synpcc7942_1119 0.67 -2.6 1,137,073 + Synpcc7942_1119 0.67 -2.2 1,137,129 - Synpcc7942_1119 0.76 +0.4 1,137,204 + Synpcc7942_1119 0.87 +1.0 1,137,214 + Synpcc7942_1119 0.88 +0.4 1,137,264 - +0.0 1,137,278 + +1.0 1,137,283 + -0.1 1,137,299 - -0.8 1,137,299 - -0.8 1,137,313 - +1.9 1,137,328 + -1.0 1,137,336 - -0.1 1,137,343 - -0.0 1,137,383 + -1.5 1,137,383 + -0.8 1,137,384 - +0.5 1,137,391 - -1.1 1,137,391 - +1.0 1,137,464 + +0.0 1,137,464 + +0.4 1,137,464 + -1.0 1,137,472 - -0.6 1,137,499 + -1.9 1,137,519 - -1.8 1,137,519 - +0.0 1,137,519 - +2.0 1,137,547 + +1.0 1,137,571 + +1.0 1,137,599 + -1.0 1,137,614 - +0.7 1,137,614 - -1.6
Or see this region's nucleotide sequence