Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt smf and act are separated by 32 nucleotides act and Synpcc7942_0705 overlap by 14 nucleotides Synpcc7942_0705 and cobL are separated by 6 nucleotides cobL and cad overlap by 20 nucleotides
Synpcc7942_0703: smf - DNA processing protein DprA, putative, at 694,072 to 695,280
smf
Synpcc7942_0704: act - Chloramphenicol O-acetyltransferase, at 695,313 to 695,948
act
Synpcc7942_0705: Synpcc7942_0705 - hypothetical protein, at 695,935 to 696,570
_0705
Synpcc7942_0706: cobL - precorrin-6B methylase, at 696,577 to 697,191
cobL
Synpcc7942_0707: cad - arginine decarboxylase, at 697,172 to 698,641
cad
Position (kb) 695
696
697 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 694.938 kb on + strand, within smf at 694.938 kb on + strand, within smf at 694.938 kb on + strand at 694.938 kb on + strand, within smf at 694.946 kb on - strand, within smf at 695.104 kb on + strand, within smf at 695.122 kb on + strand, within smf at 695.127 kb on + strand, within smf at 695.135 kb on - strand, within smf at 695.135 kb on - strand, within smf at 695.137 kb on - strand, within smf at 695.148 kb on - strand, within smf at 695.269 kb on - strand at 695.411 kb on + strand, within act at 695.556 kb on + strand, within act at 695.835 kb on + strand, within act at 696.145 kb on + strand, within Synpcc7942_0705 at 696.164 kb on + strand, within Synpcc7942_0705 at 696.209 kb on - strand, within Synpcc7942_0705 at 696.213 kb on + strand, within Synpcc7942_0705 at 696.301 kb on - strand, within Synpcc7942_0705 at 696.371 kb on - strand, within Synpcc7942_0705 at 696.387 kb on + strand, within Synpcc7942_0705 at 696.397 kb on + strand, within Synpcc7942_0705 at 696.397 kb on + strand, within Synpcc7942_0705 at 696.403 kb on - strand, within Synpcc7942_0705 at 696.464 kb on + strand, within Synpcc7942_0705 at 696.464 kb on - strand, within Synpcc7942_0705 at 696.466 kb on + strand, within Synpcc7942_0705 at 696.474 kb on - strand, within Synpcc7942_0705 at 696.474 kb on - strand at 696.474 kb on - strand, within Synpcc7942_0705 at 696.719 kb on + strand, within cobL at 696.722 kb on + strand, within cobL at 696.722 kb on + strand, within cobL at 696.724 kb on + strand, within cobL at 696.748 kb on + strand, within cobL at 696.756 kb on - strand, within cobL at 696.756 kb on - strand, within cobL at 696.770 kb on + strand, within cobL at 696.799 kb on - strand, within cobL at 696.799 kb on - strand, within cobL at 696.837 kb on + strand, within cobL at 696.845 kb on - strand, within cobL at 696.866 kb on - strand, within cobL at 696.888 kb on - strand, within cobL at 696.896 kb on + strand, within cobL at 696.923 kb on - strand, within cobL at 696.926 kb on + strand, within cobL at 696.930 kb on + strand, within cobL at 696.955 kb on - strand, within cobL at 696.983 kb on + strand, within cobL at 696.983 kb on + strand, within cobL at 697.040 kb on + strand, within cobL at 697.040 kb on - strand, within cobL at 697.055 kb on - strand, within cobL at 697.135 kb on - strand at 697.144 kb on + strand at 697.154 kb on + strand at 697.159 kb on + strand at 697.162 kb on - strand at 697.162 kb on - strand at 697.178 kb on - strand at 697.180 kb on + strand at 697.180 kb on + strand at 697.183 kb on - strand at 697.187 kb on + strand at 697.188 kb on - strand at 697.198 kb on + strand at 697.205 kb on + strand at 697.205 kb on + strand at 697.213 kb on - strand at 697.226 kb on + strand at 697.349 kb on + strand at 697.395 kb on - strand, within cad at 697.432 kb on + strand, within cad at 697.500 kb on - strand, within cad at 697.502 kb on - strand, within cad at 697.517 kb on + strand, within cad at 697.519 kb on - strand, within cad at 697.529 kb on - strand, within cad
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 694,938 + smf Synpcc7942_0703 0.72 -1.1 694,938 + smf Synpcc7942_0703 0.72 +2.1 694,938 + -2.0 694,938 + smf Synpcc7942_0703 0.72 -1.1 694,946 - smf Synpcc7942_0703 0.72 -0.7 695,104 + smf Synpcc7942_0703 0.85 -0.7 695,122 + smf Synpcc7942_0703 0.87 -0.8 695,127 + smf Synpcc7942_0703 0.87 +0.7 695,135 - smf Synpcc7942_0703 0.88 +0.0 695,135 - smf Synpcc7942_0703 0.88 -1.9 695,137 - smf Synpcc7942_0703 0.88 +0.4 695,148 - smf Synpcc7942_0703 0.89 -0.1 695,269 - -1.0 695,411 + act Synpcc7942_0704 0.15 +1.7 695,556 + act Synpcc7942_0704 0.38 +2.1 695,835 + act Synpcc7942_0704 0.82 -0.7 696,145 + Synpcc7942_0705 0.33 -0.6 696,164 + Synpcc7942_0705 0.36 +2.0 696,209 - Synpcc7942_0705 0.43 +0.4 696,213 + Synpcc7942_0705 0.44 -1.9 696,301 - Synpcc7942_0705 0.58 -2.2 696,371 - Synpcc7942_0705 0.69 -2.0 696,387 + Synpcc7942_0705 0.71 -1.5 696,397 + Synpcc7942_0705 0.73 +2.8 696,397 + Synpcc7942_0705 0.73 -1.4 696,403 - Synpcc7942_0705 0.74 +0.0 696,464 + Synpcc7942_0705 0.83 +0.3 696,464 - Synpcc7942_0705 0.83 +1.0 696,466 + Synpcc7942_0705 0.83 +1.0 696,474 - Synpcc7942_0705 0.85 -0.7 696,474 - +3.0 696,474 - Synpcc7942_0705 0.85 -0.5 696,719 + cobL Synpcc7942_0706 0.23 +1.0 696,722 + cobL Synpcc7942_0706 0.24 -0.6 696,722 + cobL Synpcc7942_0706 0.24 -1.6 696,724 + cobL Synpcc7942_0706 0.24 +1.0 696,748 + cobL Synpcc7942_0706 0.28 +1.0 696,756 - cobL Synpcc7942_0706 0.29 +0.3 696,756 - cobL Synpcc7942_0706 0.29 -0.6 696,770 + cobL Synpcc7942_0706 0.31 -0.6 696,799 - cobL Synpcc7942_0706 0.36 -1.0 696,799 - cobL Synpcc7942_0706 0.36 -2.6 696,837 + cobL Synpcc7942_0706 0.42 +0.4 696,845 - cobL Synpcc7942_0706 0.44 +1.0 696,866 - cobL Synpcc7942_0706 0.47 -1.8 696,888 - cobL Synpcc7942_0706 0.51 +1.0 696,896 + cobL Synpcc7942_0706 0.52 -0.8 696,923 - cobL Synpcc7942_0706 0.56 -1.1 696,926 + cobL Synpcc7942_0706 0.57 +2.0 696,930 + cobL Synpcc7942_0706 0.57 -1.0 696,955 - cobL Synpcc7942_0706 0.61 -2.0 696,983 + cobL Synpcc7942_0706 0.66 -1.3 696,983 + cobL Synpcc7942_0706 0.66 -1.8 697,040 + cobL Synpcc7942_0706 0.75 -2.9 697,040 - cobL Synpcc7942_0706 0.75 -0.6 697,055 - cobL Synpcc7942_0706 0.78 -2.0 697,135 - +1.0 697,144 + +0.0 697,154 + -1.0 697,159 + +1.3 697,162 - +1.0 697,162 - -0.7 697,178 - -0.6 697,180 + -1.0 697,180 + -1.3 697,183 - +2.4 697,187 + -0.5 697,188 - -0.3 697,198 + -0.6 697,205 + -1.1 697,205 + +2.2 697,213 - +1.0 697,226 + +1.0 697,349 + -1.6 697,395 - cad Synpcc7942_0707 0.15 +1.0 697,432 + cad Synpcc7942_0707 0.18 +1.0 697,500 - cad Synpcc7942_0707 0.22 -0.1 697,502 - cad Synpcc7942_0707 0.22 -0.6 697,517 + cad Synpcc7942_0707 0.23 +1.0 697,519 - cad Synpcc7942_0707 0.24 +1.0 697,529 - cad Synpcc7942_0707 0.24 -1.2
Or see this region's nucleotide sequence