Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prxQ and Synpcc7942_0643 are separated by 91 nucleotides Synpcc7942_0643 and cikA are separated by 109 nucleotides cikA and hemL are separated by 57 nucleotides
Synpcc7942_0642: prxQ - bacterioferritin comigratory protein, at 636,125 to 636,670
prxQ
Synpcc7942_0643: Synpcc7942_0643 - hypothetical protein, at 636,762 to 636,965
_0643
Synpcc7942_0644: cikA - GAF sensor hybrid histidine kinase, at 637,075 to 639,339
cikA
Synpcc7942_0645: hemL - glutamate-1-semialdehyde aminotransferase, at 639,397 to 640,758
hemL
Position (kb) 637
638
639
640 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 636.097 kb on - strand at 636.103 kb on - strand at 636.106 kb on - strand at 636.122 kb on + strand at 636.122 kb on + strand at 636.130 kb on - strand at 636.130 kb on - strand at 636.130 kb on - strand at 636.130 kb on - strand at 636.130 kb on - strand at 636.132 kb on + strand at 636.140 kb on - strand at 636.168 kb on - strand at 636.168 kb on - strand at 636.191 kb on + strand, within prxQ at 636.197 kb on - strand, within prxQ at 636.228 kb on + strand, within prxQ at 636.264 kb on + strand, within prxQ at 636.266 kb on + strand, within prxQ at 636.274 kb on - strand, within prxQ at 636.288 kb on + strand, within prxQ at 636.288 kb on + strand, within prxQ at 636.296 kb on - strand, within prxQ at 636.302 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.309 kb on + strand, within prxQ at 636.317 kb on - strand, within prxQ at 636.317 kb on - strand, within prxQ at 636.317 kb on - strand, within prxQ at 636.317 kb on - strand, within prxQ at 636.317 kb on - strand, within prxQ at 636.317 kb on - strand, within prxQ at 636.350 kb on + strand, within prxQ at 636.358 kb on + strand, within prxQ at 636.358 kb on + strand, within prxQ at 636.363 kb on + strand, within prxQ at 636.366 kb on - strand, within prxQ at 636.457 kb on - strand, within prxQ at 636.489 kb on + strand, within prxQ at 636.505 kb on + strand, within prxQ at 636.505 kb on + strand, within prxQ at 636.506 kb on + strand, within prxQ at 636.514 kb on - strand, within prxQ at 636.514 kb on - strand, within prxQ at 636.515 kb on + strand, within prxQ at 636.528 kb on + strand, within prxQ at 636.568 kb on - strand, within prxQ at 636.571 kb on + strand, within prxQ at 636.581 kb on + strand, within prxQ at 636.581 kb on + strand, within prxQ at 636.582 kb on + strand, within prxQ at 636.587 kb on + strand, within prxQ at 636.587 kb on + strand, within prxQ at 636.589 kb on - strand, within prxQ at 636.589 kb on - strand, within prxQ at 636.589 kb on - strand, within prxQ at 636.589 kb on - strand, within prxQ at 636.646 kb on - strand at 636.652 kb on + strand at 636.673 kb on + strand at 636.673 kb on + strand at 636.691 kb on - strand at 636.691 kb on - strand at 636.692 kb on + strand at 636.709 kb on - strand at 636.710 kb on - strand at 636.717 kb on + strand at 636.722 kb on + strand at 636.722 kb on + strand at 636.722 kb on + strand at 636.730 kb on - strand at 636.730 kb on - strand at 636.730 kb on - strand at 636.730 kb on - strand at 636.766 kb on - strand at 636.787 kb on - strand, within Synpcc7942_0643 at 636.792 kb on - strand, within Synpcc7942_0643 at 636.794 kb on + strand, within Synpcc7942_0643 at 636.802 kb on - strand, within Synpcc7942_0643 at 636.802 kb on - strand, within Synpcc7942_0643 at 636.802 kb on - strand, within Synpcc7942_0643 at 636.831 kb on + strand, within Synpcc7942_0643 at 636.857 kb on + strand, within Synpcc7942_0643 at 636.857 kb on + strand, within Synpcc7942_0643 at 636.867 kb on + strand, within Synpcc7942_0643 at 636.915 kb on + strand, within Synpcc7942_0643 at 636.921 kb on - strand, within Synpcc7942_0643 at 636.944 kb on + strand, within Synpcc7942_0643 at 636.944 kb on + strand, within Synpcc7942_0643 at 636.949 kb on + strand at 636.949 kb on + strand at 636.954 kb on + strand at 636.957 kb on - strand at 636.988 kb on + strand at 637.111 kb on - strand at 637.121 kb on + strand at 637.131 kb on - strand at 637.146 kb on - strand at 637.165 kb on + strand at 637.171 kb on + strand at 637.305 kb on - strand, within cikA at 637.305 kb on - strand at 637.306 kb on - strand at 637.308 kb on + strand at 637.325 kb on - strand, within cikA at 637.338 kb on - strand, within cikA at 637.338 kb on - strand, within cikA at 637.373 kb on + strand at 637.419 kb on - strand, within cikA at 637.420 kb on - strand, within cikA at 637.431 kb on + strand, within cikA at 637.619 kb on + strand at 637.995 kb on - strand at 638.076 kb on - strand, within cikA at 638.080 kb on + strand, within cikA at 638.096 kb on - strand, within cikA at 638.096 kb on - strand at 638.113 kb on + strand, within cikA at 638.115 kb on + strand at 638.115 kb on + strand at 638.123 kb on - strand, within cikA at 638.123 kb on - strand, within cikA at 638.123 kb on - strand, within cikA at 638.123 kb on - strand, within cikA at 638.123 kb on - strand, within cikA at 638.135 kb on - strand at 638.157 kb on - strand, within cikA at 638.261 kb on + strand, within cikA at 638.274 kb on - strand, within cikA at 638.274 kb on - strand at 638.274 kb on - strand, within cikA at 638.274 kb on - strand, within cikA at 638.289 kb on + strand, within cikA at 638.319 kb on - strand at 638.368 kb on + strand, within cikA at 638.481 kb on + strand, within cikA at 638.489 kb on - strand, within cikA at 638.490 kb on - strand, within cikA at 638.552 kb on + strand, within cikA at 638.634 kb on + strand, within cikA at 638.643 kb on - strand, within cikA at 638.666 kb on - strand, within cikA at 638.737 kb on - strand, within cikA at 638.794 kb on + strand at 638.794 kb on + strand at 638.794 kb on + strand, within cikA at 638.802 kb on - strand, within cikA at 638.802 kb on - strand, within cikA at 638.866 kb on + strand, within cikA at 638.866 kb on + strand at 638.874 kb on - strand at 638.924 kb on + strand at 638.924 kb on + strand at 638.924 kb on + strand, within cikA at 638.924 kb on + strand, within cikA at 638.924 kb on + strand at 638.932 kb on - strand, within cikA at 638.932 kb on - strand, within cikA at 638.932 kb on - strand, within cikA at 638.932 kb on - strand, within cikA at 638.932 kb on - strand, within cikA at 638.932 kb on - strand, within cikA at 638.982 kb on + strand at 638.982 kb on + strand, within cikA at 639.020 kb on - strand, within cikA at 639.026 kb on + strand, within cikA at 639.034 kb on - strand, within cikA at 639.034 kb on - strand, within cikA at 639.034 kb on - strand, within cikA at 639.061 kb on + strand, within cikA at 639.061 kb on + strand, within cikA at 639.061 kb on + strand at 639.061 kb on + strand, within cikA at 639.070 kb on - strand, within cikA at 639.105 kb on + strand, within cikA at 639.131 kb on + strand at 639.139 kb on - strand at 639.152 kb on + strand at 639.168 kb on - strand at 639.168 kb on - strand at 639.217 kb on + strand at 639.229 kb on + strand at 639.263 kb on + strand at 639.276 kb on + strand at 639.276 kb on + strand at 639.301 kb on - strand at 639.312 kb on + strand at 639.326 kb on + strand at 639.348 kb on + strand at 639.354 kb on - strand at 639.356 kb on + strand at 639.358 kb on + strand at 639.364 kb on - strand at 639.365 kb on + strand at 639.377 kb on + strand at 639.400 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 636,097 - -0.6 636,103 - -0.2 636,106 - -1.7 636,122 + +1.0 636,122 + -1.2 636,130 - -0.9 636,130 - +0.2 636,130 - -0.9 636,130 - -0.9 636,130 - -2.2 636,132 + -1.0 636,140 - -1.7 636,168 - -0.3 636,168 - -0.6 636,191 + prxQ Synpcc7942_0642 0.12 -1.1 636,197 - prxQ Synpcc7942_0642 0.13 -1.0 636,228 + prxQ Synpcc7942_0642 0.19 -0.8 636,264 + prxQ Synpcc7942_0642 0.25 +1.0 636,266 + prxQ Synpcc7942_0642 0.26 +0.4 636,274 - prxQ Synpcc7942_0642 0.27 +1.6 636,288 + prxQ Synpcc7942_0642 0.30 +1.0 636,288 + prxQ Synpcc7942_0642 0.30 +0.6 636,296 - prxQ Synpcc7942_0642 0.31 +1.0 636,302 + prxQ Synpcc7942_0642 0.32 -1.1 636,309 + prxQ Synpcc7942_0642 0.34 -1.0 636,309 + prxQ Synpcc7942_0642 0.34 -1.6 636,309 + prxQ Synpcc7942_0642 0.34 -0.6 636,309 + prxQ Synpcc7942_0642 0.34 -1.1 636,309 + prxQ Synpcc7942_0642 0.34 +1.0 636,309 + prxQ Synpcc7942_0642 0.34 -0.9 636,309 + prxQ Synpcc7942_0642 0.34 -0.4 636,309 + prxQ Synpcc7942_0642 0.34 -0.6 636,309 + prxQ Synpcc7942_0642 0.34 +0.6 636,317 - prxQ Synpcc7942_0642 0.35 +0.4 636,317 - prxQ Synpcc7942_0642 0.35 -1.2 636,317 - prxQ Synpcc7942_0642 0.35 -0.1 636,317 - prxQ Synpcc7942_0642 0.35 -0.2 636,317 - prxQ Synpcc7942_0642 0.35 -0.8 636,317 - prxQ Synpcc7942_0642 0.35 -0.2 636,350 + prxQ Synpcc7942_0642 0.41 -1.6 636,358 + prxQ Synpcc7942_0642 0.43 -1.1 636,358 + prxQ Synpcc7942_0642 0.43 -1.6 636,363 + prxQ Synpcc7942_0642 0.44 +1.0 636,366 - prxQ Synpcc7942_0642 0.44 +1.0 636,457 - prxQ Synpcc7942_0642 0.61 +2.6 636,489 + prxQ Synpcc7942_0642 0.67 -2.5 636,505 + prxQ Synpcc7942_0642 0.70 +2.0 636,505 + prxQ Synpcc7942_0642 0.70 -1.3 636,506 + prxQ Synpcc7942_0642 0.70 -0.0 636,514 - prxQ Synpcc7942_0642 0.71 +1.0 636,514 - prxQ Synpcc7942_0642 0.71 -1.6 636,515 + prxQ Synpcc7942_0642 0.71 -0.8 636,528 + prxQ Synpcc7942_0642 0.74 -0.9 636,568 - prxQ Synpcc7942_0642 0.81 +0.4 636,571 + prxQ Synpcc7942_0642 0.82 -1.3 636,581 + prxQ Synpcc7942_0642 0.84 +1.2 636,581 + prxQ Synpcc7942_0642 0.84 +2.3 636,582 + prxQ Synpcc7942_0642 0.84 +1.0 636,587 + prxQ Synpcc7942_0642 0.85 -1.5 636,587 + prxQ Synpcc7942_0642 0.85 +2.1 636,589 - prxQ Synpcc7942_0642 0.85 -0.8 636,589 - prxQ Synpcc7942_0642 0.85 -0.4 636,589 - prxQ Synpcc7942_0642 0.85 +1.0 636,589 - prxQ Synpcc7942_0642 0.85 +0.5 636,646 - +0.3 636,652 + -0.6 636,673 + -2.2 636,673 + -1.4 636,691 - -0.2 636,691 - -0.9 636,692 + -0.9 636,709 - -1.2 636,710 - -1.7 636,717 + -1.2 636,722 + -1.0 636,722 + +0.2 636,722 + +0.4 636,730 - -1.0 636,730 - -0.6 636,730 - -1.7 636,730 - +0.7 636,766 - +1.0 636,787 - Synpcc7942_0643 0.12 -1.2 636,792 - Synpcc7942_0643 0.15 +2.2 636,794 + Synpcc7942_0643 0.16 +0.2 636,802 - Synpcc7942_0643 0.20 +1.0 636,802 - Synpcc7942_0643 0.20 +1.0 636,802 - Synpcc7942_0643 0.20 +0.2 636,831 + Synpcc7942_0643 0.34 -0.2 636,857 + Synpcc7942_0643 0.47 -0.2 636,857 + Synpcc7942_0643 0.47 +1.0 636,867 + Synpcc7942_0643 0.51 -0.4 636,915 + Synpcc7942_0643 0.75 -1.3 636,921 - Synpcc7942_0643 0.78 -0.3 636,944 + Synpcc7942_0643 0.89 +2.6 636,944 + Synpcc7942_0643 0.89 -1.1 636,949 + +1.0 636,949 + -0.4 636,954 + +1.0 636,957 - +0.4 636,988 + -0.2 637,111 - -1.0 637,121 + +1.0 637,131 - -0.0 637,146 - +1.0 637,165 + -0.8 637,171 + -0.0 637,305 - cikA Synpcc7942_0644 0.10 -0.4 637,305 - +1.0 637,306 - -0.0 637,308 + -0.0 637,325 - cikA Synpcc7942_0644 0.11 -0.0 637,338 - cikA Synpcc7942_0644 0.12 -0.6 637,338 - cikA Synpcc7942_0644 0.12 -0.0 637,373 + +1.0 637,419 - cikA Synpcc7942_0644 0.15 +1.0 637,420 - cikA Synpcc7942_0644 0.15 +1.0 637,431 + cikA Synpcc7942_0644 0.16 -1.0 637,619 + +0.4 637,995 - +1.0 638,076 - cikA Synpcc7942_0644 0.44 -2.1 638,080 + cikA Synpcc7942_0644 0.44 +1.0 638,096 - cikA Synpcc7942_0644 0.45 -0.0 638,096 - +1.0 638,113 + cikA Synpcc7942_0644 0.46 -0.6 638,115 + +0.6 638,115 + +1.0 638,123 - cikA Synpcc7942_0644 0.46 -2.5 638,123 - cikA Synpcc7942_0644 0.46 -0.0 638,123 - cikA Synpcc7942_0644 0.46 -0.0 638,123 - cikA Synpcc7942_0644 0.46 -0.6 638,123 - cikA Synpcc7942_0644 0.46 -3.3 638,135 - -0.6 638,157 - cikA Synpcc7942_0644 0.48 -0.0 638,261 + cikA Synpcc7942_0644 0.52 -0.6 638,274 - cikA Synpcc7942_0644 0.53 +1.0 638,274 - +1.0 638,274 - cikA Synpcc7942_0644 0.53 -0.0 638,274 - cikA Synpcc7942_0644 0.53 +1.0 638,289 + cikA Synpcc7942_0644 0.54 -0.7 638,319 - +1.0 638,368 + cikA Synpcc7942_0644 0.57 +1.0 638,481 + cikA Synpcc7942_0644 0.62 -0.9 638,489 - cikA Synpcc7942_0644 0.62 +1.0 638,490 - cikA Synpcc7942_0644 0.62 -0.0 638,552 + cikA Synpcc7942_0644 0.65 +1.0 638,634 + cikA Synpcc7942_0644 0.69 -0.6 638,643 - cikA Synpcc7942_0644 0.69 +1.0 638,666 - cikA Synpcc7942_0644 0.70 +1.0 638,737 - cikA Synpcc7942_0644 0.73 -1.9 638,794 + +2.0 638,794 + -0.6 638,794 + cikA Synpcc7942_0644 0.76 -0.0 638,802 - cikA Synpcc7942_0644 0.76 -1.0 638,802 - cikA Synpcc7942_0644 0.76 +1.0 638,866 + cikA Synpcc7942_0644 0.79 -0.0 638,866 + +1.0 638,874 - +1.0 638,924 + -0.0 638,924 + -0.3 638,924 + cikA Synpcc7942_0644 0.82 +1.0 638,924 + cikA Synpcc7942_0644 0.82 +1.0 638,924 + -0.6 638,932 - cikA Synpcc7942_0644 0.82 -0.0 638,932 - cikA Synpcc7942_0644 0.82 +1.0 638,932 - cikA Synpcc7942_0644 0.82 -0.0 638,932 - cikA Synpcc7942_0644 0.82 -0.0 638,932 - cikA Synpcc7942_0644 0.82 -0.0 638,932 - cikA Synpcc7942_0644 0.82 +1.0 638,982 + +1.0 638,982 + cikA Synpcc7942_0644 0.84 -1.0 639,020 - cikA Synpcc7942_0644 0.86 -0.0 639,026 + cikA Synpcc7942_0644 0.86 -2.3 639,034 - cikA Synpcc7942_0644 0.86 +1.0 639,034 - cikA Synpcc7942_0644 0.86 -0.5 639,034 - cikA Synpcc7942_0644 0.86 +1.0 639,061 + cikA Synpcc7942_0644 0.88 -0.3 639,061 + cikA Synpcc7942_0644 0.88 +1.0 639,061 + +1.0 639,061 + cikA Synpcc7942_0644 0.88 +0.4 639,070 - cikA Synpcc7942_0644 0.88 +1.0 639,105 + cikA Synpcc7942_0644 0.90 -0.6 639,131 + -1.0 639,139 - -0.0 639,152 + +2.0 639,168 - +1.0 639,168 - +2.0 639,217 + -0.0 639,229 + -0.0 639,263 + -0.3 639,276 + -0.6 639,276 + -1.5 639,301 - +1.0 639,312 + -0.6 639,326 + +2.0 639,348 + -0.9 639,354 - -0.0 639,356 + +0.1 639,358 + +1.0 639,364 - +0.8 639,365 + -0.7 639,377 + +0.4 639,400 - +0.2
Or see this region's nucleotide sequence