Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metY and metA are separated by 48 nucleotides metA and Synpcc7942_0372 are separated by 37 nucleotides Synpcc7942_0372 and Synpcc7942_0373 overlap by 116 nucleotides
Synpcc7942_0370: metY - O-acetylhomoserine/O-acetylserine sulfhydrylase, at 363,763 to 365,067
metY
Synpcc7942_0371: metA - homoserine O-succinyltransferase, at 365,116 to 366,015
metA
Synpcc7942_0372: Synpcc7942_0372 - hypothetical protein, at 366,053 to 366,853
_0372
Synpcc7942_0373: Synpcc7942_0373 - hypothetical protein, at 366,738 to 368,024
_0373
Position (kb) 365
366
367 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 364.117 kb on + strand, within metY at 364.133 kb on + strand, within metY at 364.206 kb on + strand, within metY at 364.206 kb on + strand at 364.214 kb on - strand, within metY at 364.214 kb on - strand, within metY at 364.215 kb on - strand at 364.231 kb on + strand, within metY at 364.238 kb on - strand, within metY at 364.243 kb on + strand, within metY at 364.244 kb on - strand, within metY at 364.283 kb on + strand, within metY at 364.300 kb on + strand, within metY at 364.324 kb on + strand, within metY at 364.329 kb on + strand, within metY at 364.337 kb on + strand, within metY at 364.337 kb on - strand, within metY at 364.337 kb on - strand, within metY at 364.345 kb on - strand, within metY at 364.363 kb on + strand, within metY at 364.363 kb on + strand, within metY at 364.417 kb on - strand, within metY at 364.472 kb on + strand, within metY at 364.480 kb on - strand, within metY at 364.538 kb on + strand, within metY at 364.546 kb on - strand, within metY at 364.546 kb on - strand at 364.548 kb on + strand, within metY at 364.573 kb on + strand, within metY at 364.581 kb on - strand, within metY at 364.600 kb on - strand, within metY at 364.624 kb on + strand, within metY at 364.631 kb on - strand, within metY at 364.632 kb on - strand, within metY at 364.650 kb on + strand, within metY at 364.650 kb on - strand, within metY at 364.705 kb on - strand, within metY at 364.733 kb on + strand, within metY at 364.733 kb on + strand, within metY at 364.756 kb on + strand, within metY at 364.756 kb on + strand, within metY at 364.764 kb on - strand, within metY at 364.764 kb on - strand, within metY at 364.788 kb on - strand, within metY at 364.788 kb on - strand, within metY at 364.801 kb on + strand, within metY at 364.809 kb on - strand, within metY at 364.809 kb on - strand, within metY at 364.809 kb on - strand, within metY at 364.809 kb on - strand, within metY at 364.831 kb on - strand, within metY at 364.858 kb on + strand, within metY at 364.928 kb on + strand, within metY at 364.936 kb on - strand, within metY at 364.936 kb on - strand, within metY at 364.959 kb on - strand at 364.966 kb on + strand at 364.971 kb on - strand at 364.989 kb on + strand at 364.999 kb on + strand at 365.008 kb on + strand at 365.008 kb on - strand at 365.009 kb on + strand at 365.013 kb on + strand at 365.019 kb on + strand at 365.030 kb on + strand at 365.041 kb on + strand at 365.071 kb on + strand at 365.084 kb on - strand at 365.099 kb on + strand at 365.114 kb on - strand at 365.187 kb on - strand at 365.248 kb on - strand, within metA at 365.257 kb on - strand, within metA at 365.287 kb on + strand, within metA at 365.297 kb on - strand, within metA at 365.317 kb on + strand, within metA at 365.360 kb on - strand, within metA at 365.387 kb on - strand, within metA at 365.428 kb on - strand at 365.477 kb on + strand, within metA at 365.485 kb on - strand, within metA at 365.507 kb on + strand, within metA at 365.764 kb on - strand, within metA at 365.773 kb on + strand, within metA at 365.805 kb on - strand, within metA at 365.819 kb on - strand, within metA at 365.827 kb on - strand, within metA at 365.945 kb on + strand at 365.954 kb on + strand at 366.175 kb on - strand, within Synpcc7942_0372 at 366.186 kb on - strand, within Synpcc7942_0372 at 366.218 kb on - strand, within Synpcc7942_0372 at 366.233 kb on - strand, within Synpcc7942_0372 at 366.246 kb on - strand, within Synpcc7942_0372 at 366.280 kb on + strand, within Synpcc7942_0372 at 366.340 kb on - strand, within Synpcc7942_0372 at 366.403 kb on - strand, within Synpcc7942_0372 at 366.420 kb on - strand, within Synpcc7942_0372 at 366.432 kb on - strand, within Synpcc7942_0372 at 366.452 kb on + strand, within Synpcc7942_0372 at 366.490 kb on - strand, within Synpcc7942_0372 at 366.518 kb on - strand, within Synpcc7942_0372 at 366.526 kb on - strand, within Synpcc7942_0372 at 366.529 kb on + strand, within Synpcc7942_0372 at 366.537 kb on - strand, within Synpcc7942_0372 at 366.641 kb on + strand, within Synpcc7942_0372 at 366.641 kb on + strand, within Synpcc7942_0372 at 366.641 kb on + strand, within Synpcc7942_0372 at 366.649 kb on - strand, within Synpcc7942_0372 at 366.649 kb on - strand, within Synpcc7942_0372 at 366.649 kb on - strand, within Synpcc7942_0372 at 366.649 kb on - strand, within Synpcc7942_0372 at 366.649 kb on - strand, within Synpcc7942_0372 at 366.684 kb on + strand, within Synpcc7942_0372 at 366.685 kb on + strand, within Synpcc7942_0372 at 366.720 kb on + strand, within Synpcc7942_0372 at 366.720 kb on + strand, within Synpcc7942_0372 at 366.730 kb on + strand, within Synpcc7942_0372 at 366.742 kb on + strand at 366.742 kb on + strand at 366.742 kb on + strand at 366.742 kb on + strand at 366.750 kb on - strand at 366.776 kb on + strand at 366.788 kb on + strand at 366.789 kb on + strand at 366.799 kb on - strand at 366.820 kb on + strand at 366.847 kb on + strand at 366.847 kb on + strand at 366.847 kb on + strand at 367.009 kb on - strand, within Synpcc7942_0373
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 364,117 + metY Synpcc7942_0370 0.27 +0.8 364,133 + metY Synpcc7942_0370 0.28 +1.4 364,206 + metY Synpcc7942_0370 0.34 +0.2 364,206 + +0.1 364,214 - metY Synpcc7942_0370 0.35 -0.3 364,214 - metY Synpcc7942_0370 0.35 +1.0 364,215 - -0.3 364,231 + metY Synpcc7942_0370 0.36 +1.0 364,238 - metY Synpcc7942_0370 0.36 +0.4 364,243 + metY Synpcc7942_0370 0.37 -0.7 364,244 - metY Synpcc7942_0370 0.37 -0.8 364,283 + metY Synpcc7942_0370 0.40 -1.7 364,300 + metY Synpcc7942_0370 0.41 -0.4 364,324 + metY Synpcc7942_0370 0.43 -0.0 364,329 + metY Synpcc7942_0370 0.43 -1.0 364,337 + metY Synpcc7942_0370 0.44 -1.0 364,337 - metY Synpcc7942_0370 0.44 +1.0 364,337 - metY Synpcc7942_0370 0.44 -0.0 364,345 - metY Synpcc7942_0370 0.45 +1.0 364,363 + metY Synpcc7942_0370 0.46 -0.3 364,363 + metY Synpcc7942_0370 0.46 -1.0 364,417 - metY Synpcc7942_0370 0.50 +2.0 364,472 + metY Synpcc7942_0370 0.54 -1.7 364,480 - metY Synpcc7942_0370 0.55 -0.0 364,538 + metY Synpcc7942_0370 0.59 -0.0 364,546 - metY Synpcc7942_0370 0.60 -0.3 364,546 - +1.0 364,548 + metY Synpcc7942_0370 0.60 -1.1 364,573 + metY Synpcc7942_0370 0.62 -0.8 364,581 - metY Synpcc7942_0370 0.63 -0.3 364,600 - metY Synpcc7942_0370 0.64 -0.8 364,624 + metY Synpcc7942_0370 0.66 -0.8 364,631 - metY Synpcc7942_0370 0.67 +0.2 364,632 - metY Synpcc7942_0370 0.67 -2.9 364,650 + metY Synpcc7942_0370 0.68 -1.8 364,650 - metY Synpcc7942_0370 0.68 +2.0 364,705 - metY Synpcc7942_0370 0.72 -0.6 364,733 + metY Synpcc7942_0370 0.74 -0.6 364,733 + metY Synpcc7942_0370 0.74 -0.3 364,756 + metY Synpcc7942_0370 0.76 -1.3 364,756 + metY Synpcc7942_0370 0.76 -1.0 364,764 - metY Synpcc7942_0370 0.77 -0.3 364,764 - metY Synpcc7942_0370 0.77 -1.3 364,788 - metY Synpcc7942_0370 0.79 +0.7 364,788 - metY Synpcc7942_0370 0.79 +2.0 364,801 + metY Synpcc7942_0370 0.80 +0.1 364,809 - metY Synpcc7942_0370 0.80 -1.6 364,809 - metY Synpcc7942_0370 0.80 -0.1 364,809 - metY Synpcc7942_0370 0.80 -0.3 364,809 - metY Synpcc7942_0370 0.80 +2.4 364,831 - metY Synpcc7942_0370 0.82 -0.8 364,858 + metY Synpcc7942_0370 0.84 -1.0 364,928 + metY Synpcc7942_0370 0.89 -0.0 364,936 - metY Synpcc7942_0370 0.90 -0.3 364,936 - metY Synpcc7942_0370 0.90 -0.8 364,959 - +2.0 364,966 + -0.5 364,971 - +1.0 364,989 + -1.1 364,999 + -2.0 365,008 + +1.0 365,008 - +0.9 365,009 + +1.0 365,013 + +1.0 365,019 + -1.0 365,030 + -0.7 365,041 + +0.3 365,071 + +1.4 365,084 - +2.0 365,099 + -0.8 365,114 - -1.8 365,187 - +1.7 365,248 - metA Synpcc7942_0371 0.15 +1.3 365,257 - metA Synpcc7942_0371 0.16 -2.3 365,287 + metA Synpcc7942_0371 0.19 -1.3 365,297 - metA Synpcc7942_0371 0.20 -1.1 365,317 + metA Synpcc7942_0371 0.22 -0.3 365,360 - metA Synpcc7942_0371 0.27 -1.8 365,387 - metA Synpcc7942_0371 0.30 +2.3 365,428 - -2.2 365,477 + metA Synpcc7942_0371 0.40 -0.6 365,485 - metA Synpcc7942_0371 0.41 -2.5 365,507 + metA Synpcc7942_0371 0.43 +2.0 365,764 - metA Synpcc7942_0371 0.72 -1.0 365,773 + metA Synpcc7942_0371 0.73 +1.0 365,805 - metA Synpcc7942_0371 0.77 -1.3 365,819 - metA Synpcc7942_0371 0.78 -1.6 365,827 - metA Synpcc7942_0371 0.79 -0.3 365,945 + -1.1 365,954 + -1.1 366,175 - Synpcc7942_0372 0.15 -0.3 366,186 - Synpcc7942_0372 0.17 +0.3 366,218 - Synpcc7942_0372 0.21 -0.4 366,233 - Synpcc7942_0372 0.22 -0.0 366,246 - Synpcc7942_0372 0.24 +0.2 366,280 + Synpcc7942_0372 0.28 -0.6 366,340 - Synpcc7942_0372 0.36 +2.0 366,403 - Synpcc7942_0372 0.44 -0.9 366,420 - Synpcc7942_0372 0.46 +1.0 366,432 - Synpcc7942_0372 0.47 +2.3 366,452 + Synpcc7942_0372 0.50 -0.5 366,490 - Synpcc7942_0372 0.55 -1.6 366,518 - Synpcc7942_0372 0.58 +1.0 366,526 - Synpcc7942_0372 0.59 -0.9 366,529 + Synpcc7942_0372 0.59 +1.0 366,537 - Synpcc7942_0372 0.60 -1.6 366,641 + Synpcc7942_0372 0.73 +0.3 366,641 + Synpcc7942_0372 0.73 -0.8 366,641 + Synpcc7942_0372 0.73 +1.0 366,649 - Synpcc7942_0372 0.74 -0.8 366,649 - Synpcc7942_0372 0.74 +0.2 366,649 - Synpcc7942_0372 0.74 +1.0 366,649 - Synpcc7942_0372 0.74 -0.9 366,649 - Synpcc7942_0372 0.74 -0.8 366,684 + Synpcc7942_0372 0.79 +0.3 366,685 + Synpcc7942_0372 0.79 +0.0 366,720 + Synpcc7942_0372 0.83 -0.6 366,720 + Synpcc7942_0372 0.83 -1.0 366,730 + Synpcc7942_0372 0.85 +0.2 366,742 + +0.4 366,742 + -0.6 366,742 + +1.0 366,742 + -1.5 366,750 - -0.4 366,776 + -0.7 366,788 + -1.8 366,789 + +0.9 366,799 - -1.5 366,820 + -0.8 366,847 + -2.0 366,847 + -1.0 366,847 + -1.0 367,009 - Synpcc7942_0373 0.21 +2.0
Or see this region's nucleotide sequence