Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt napA and chpX are separated by 32 nucleotides chpX and ndhF4 are separated by 30 nucleotides
Synpcc7942_0307: napA - Na+/H+ antiporter, at 304,710 to 305,936
napA
Synpcc7942_0308: chpX - CO2 hydration protein, at 305,969 to 307,108
chpX
Synpcc7942_0309: ndhF4 - NAD(P)H-quinone oxidoreductase subunit F, at 307,139 to 309,043
ndhF4
Position (kb) 305
306
307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 304.970 kb on - strand, within napA at 305.040 kb on + strand, within napA at 305.040 kb on + strand, within napA at 305.040 kb on + strand, within napA at 305.048 kb on - strand, within napA at 305.048 kb on - strand, within napA at 305.065 kb on + strand, within napA at 305.073 kb on - strand, within napA at 305.075 kb on - strand, within napA at 305.078 kb on + strand at 305.085 kb on - strand, within napA at 305.108 kb on - strand, within napA at 305.177 kb on + strand, within napA at 305.199 kb on - strand, within napA at 305.205 kb on + strand, within napA at 305.227 kb on + strand, within napA at 305.247 kb on + strand, within napA at 305.267 kb on - strand, within napA at 305.291 kb on - strand at 305.326 kb on - strand, within napA at 305.328 kb on + strand, within napA at 305.333 kb on + strand, within napA at 305.333 kb on + strand, within napA at 305.341 kb on - strand, within napA at 305.341 kb on - strand, within napA at 305.374 kb on - strand at 305.393 kb on + strand, within napA at 305.418 kb on - strand, within napA at 305.421 kb on + strand, within napA at 305.461 kb on + strand, within napA at 305.506 kb on - strand, within napA at 305.523 kb on + strand, within napA at 305.523 kb on + strand, within napA at 305.525 kb on - strand, within napA at 305.531 kb on + strand, within napA at 305.531 kb on + strand, within napA at 305.531 kb on + strand, within napA at 305.531 kb on + strand, within napA at 305.531 kb on + strand, within napA at 305.531 kb on + strand, within napA at 305.531 kb on - strand, within napA at 305.539 kb on - strand, within napA at 305.539 kb on - strand, within napA at 305.565 kb on + strand, within napA at 305.571 kb on + strand at 305.571 kb on + strand, within napA at 305.668 kb on + strand, within napA at 305.668 kb on + strand, within napA at 305.678 kb on + strand, within napA at 305.718 kb on + strand, within napA at 305.721 kb on - strand, within napA at 305.744 kb on - strand, within napA at 305.752 kb on - strand, within napA at 305.778 kb on - strand, within napA at 305.788 kb on - strand, within napA at 305.788 kb on - strand, within napA at 305.788 kb on - strand, within napA at 305.792 kb on + strand, within napA at 305.831 kb on + strand at 305.839 kb on - strand at 305.839 kb on - strand at 305.852 kb on - strand at 305.852 kb on - strand at 305.852 kb on - strand at 305.877 kb on + strand at 305.880 kb on - strand at 305.933 kb on + strand at 305.939 kb on - strand at 305.953 kb on - strand at 305.960 kb on + strand at 305.971 kb on + strand at 306.003 kb on + strand at 306.003 kb on + strand at 306.006 kb on - strand at 306.011 kb on - strand at 306.011 kb on - strand at 306.018 kb on + strand at 306.032 kb on - strand at 306.061 kb on + strand at 306.093 kb on - strand, within chpX at 306.165 kb on + strand, within chpX at 306.186 kb on + strand, within chpX at 306.227 kb on + strand, within chpX at 306.227 kb on + strand, within chpX at 306.264 kb on + strand, within chpX at 306.342 kb on + strand, within chpX at 306.352 kb on - strand, within chpX at 306.377 kb on + strand, within chpX at 306.398 kb on + strand, within chpX at 306.398 kb on + strand, within chpX at 306.400 kb on - strand, within chpX at 306.412 kb on + strand, within chpX at 306.597 kb on - strand, within chpX at 306.606 kb on + strand, within chpX at 306.616 kb on + strand, within chpX at 306.629 kb on + strand, within chpX at 306.637 kb on - strand, within chpX at 306.637 kb on - strand, within chpX at 306.675 kb on + strand, within chpX at 306.687 kb on + strand, within chpX at 306.691 kb on + strand, within chpX at 306.748 kb on + strand, within chpX at 306.804 kb on + strand, within chpX at 306.819 kb on - strand, within chpX at 306.832 kb on - strand, within chpX at 306.862 kb on + strand at 306.941 kb on - strand, within chpX at 306.959 kb on + strand, within chpX at 306.970 kb on + strand, within chpX at 306.972 kb on - strand, within chpX at 306.978 kb on - strand, within chpX at 306.989 kb on + strand, within chpX at 306.994 kb on - strand, within chpX at 306.994 kb on - strand, within chpX at 307.017 kb on + strand at 307.035 kb on - strand at 307.084 kb on - strand at 307.085 kb on + strand at 307.087 kb on + strand at 307.088 kb on - strand at 307.095 kb on - strand at 307.139 kb on - strand at 307.169 kb on - strand at 307.169 kb on - strand at 307.195 kb on + strand at 307.209 kb on - strand at 307.209 kb on - strand at 307.213 kb on + strand at 307.216 kb on - strand at 307.286 kb on - strand at 307.286 kb on - strand at 307.296 kb on + strand at 307.323 kb on + strand at 307.331 kb on - strand, within ndhF4 at 307.331 kb on - strand, within ndhF4 at 307.356 kb on + strand, within ndhF4 at 307.356 kb on + strand, within ndhF4 at 307.356 kb on + strand, within ndhF4 at 307.356 kb on + strand, within ndhF4 at 307.364 kb on - strand, within ndhF4 at 307.364 kb on - strand, within ndhF4 at 307.384 kb on - strand, within ndhF4 at 307.385 kb on + strand, within ndhF4 at 307.413 kb on - strand, within ndhF4 at 307.453 kb on - strand at 307.453 kb on - strand, within ndhF4 at 307.455 kb on + strand, within ndhF4 at 307.455 kb on + strand, within ndhF4 at 307.506 kb on - strand, within ndhF4 at 307.512 kb on - strand, within ndhF4 at 307.583 kb on + strand, within ndhF4 at 307.596 kb on - strand, within ndhF4 at 307.680 kb on + strand, within ndhF4 at 307.688 kb on + strand, within ndhF4 at 307.688 kb on - strand, within ndhF4 at 307.690 kb on + strand, within ndhF4 at 307.718 kb on + strand at 307.726 kb on - strand, within ndhF4 at 307.804 kb on + strand, within ndhF4 at 307.878 kb on + strand, within ndhF4 at 307.881 kb on - strand, within ndhF4 at 307.888 kb on - strand, within ndhF4 at 307.927 kb on + strand, within ndhF4 at 308.002 kb on + strand, within ndhF4 at 308.004 kb on + strand, within ndhF4 at 308.004 kb on + strand, within ndhF4 at 308.012 kb on - strand, within ndhF4 at 308.028 kb on + strand, within ndhF4 at 308.038 kb on + strand, within ndhF4 at 308.046 kb on - strand, within ndhF4 at 308.046 kb on - strand, within ndhF4 at 308.067 kb on + strand, within ndhF4 at 308.067 kb on - strand, within ndhF4 at 308.099 kb on - strand, within ndhF4 at 308.100 kb on - strand, within ndhF4
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 304,970 - napA Synpcc7942_0307 0.21 -0.6 305,040 + napA Synpcc7942_0307 0.27 +0.1 305,040 + napA Synpcc7942_0307 0.27 +0.6 305,040 + napA Synpcc7942_0307 0.27 -0.6 305,048 - napA Synpcc7942_0307 0.28 -0.0 305,048 - napA Synpcc7942_0307 0.28 -1.4 305,065 + napA Synpcc7942_0307 0.29 +0.5 305,073 - napA Synpcc7942_0307 0.30 -1.3 305,075 - napA Synpcc7942_0307 0.30 -0.0 305,078 + -0.9 305,085 - napA Synpcc7942_0307 0.31 -1.4 305,108 - napA Synpcc7942_0307 0.32 -0.5 305,177 + napA Synpcc7942_0307 0.38 +0.4 305,199 - napA Synpcc7942_0307 0.40 -1.1 305,205 + napA Synpcc7942_0307 0.40 +1.1 305,227 + napA Synpcc7942_0307 0.42 +2.0 305,247 + napA Synpcc7942_0307 0.44 -0.5 305,267 - napA Synpcc7942_0307 0.45 -0.8 305,291 - -1.6 305,326 - napA Synpcc7942_0307 0.50 -1.2 305,328 + napA Synpcc7942_0307 0.50 -0.9 305,333 + napA Synpcc7942_0307 0.51 -1.7 305,333 + napA Synpcc7942_0307 0.51 -0.9 305,341 - napA Synpcc7942_0307 0.51 -0.6 305,341 - napA Synpcc7942_0307 0.51 -1.6 305,374 - -2.3 305,393 + napA Synpcc7942_0307 0.56 -0.6 305,418 - napA Synpcc7942_0307 0.58 -1.3 305,421 + napA Synpcc7942_0307 0.58 -2.7 305,461 + napA Synpcc7942_0307 0.61 +0.8 305,506 - napA Synpcc7942_0307 0.65 -1.0 305,523 + napA Synpcc7942_0307 0.66 +1.8 305,523 + napA Synpcc7942_0307 0.66 +3.3 305,525 - napA Synpcc7942_0307 0.66 +1.1 305,531 + napA Synpcc7942_0307 0.67 -0.1 305,531 + napA Synpcc7942_0307 0.67 +0.1 305,531 + napA Synpcc7942_0307 0.67 -0.8 305,531 + napA Synpcc7942_0307 0.67 +0.2 305,531 + napA Synpcc7942_0307 0.67 +1.8 305,531 + napA Synpcc7942_0307 0.67 -0.0 305,531 - napA Synpcc7942_0307 0.67 -0.9 305,539 - napA Synpcc7942_0307 0.68 -0.1 305,539 - napA Synpcc7942_0307 0.68 -0.8 305,565 + napA Synpcc7942_0307 0.70 -0.5 305,571 + +1.0 305,571 + napA Synpcc7942_0307 0.70 -0.7 305,668 + napA Synpcc7942_0307 0.78 +2.7 305,668 + napA Synpcc7942_0307 0.78 +1.6 305,678 + napA Synpcc7942_0307 0.79 -2.1 305,718 + napA Synpcc7942_0307 0.82 -1.2 305,721 - napA Synpcc7942_0307 0.82 +1.0 305,744 - napA Synpcc7942_0307 0.84 -0.0 305,752 - napA Synpcc7942_0307 0.85 +1.6 305,778 - napA Synpcc7942_0307 0.87 +0.8 305,788 - napA Synpcc7942_0307 0.88 -0.6 305,788 - napA Synpcc7942_0307 0.88 -0.1 305,788 - napA Synpcc7942_0307 0.88 -0.0 305,792 + napA Synpcc7942_0307 0.88 +1.8 305,831 + +0.2 305,839 - +0.3 305,839 - -0.3 305,852 - +1.0 305,852 - +1.6 305,852 - -1.9 305,877 + -0.6 305,880 - -2.9 305,933 + -0.6 305,939 - +1.6 305,953 - -1.5 305,960 + -0.6 305,971 + -1.4 306,003 + -0.9 306,003 + -0.7 306,006 - +1.0 306,011 - +1.0 306,011 - -1.9 306,018 + -0.6 306,032 - +2.8 306,061 + +3.0 306,093 - chpX Synpcc7942_0308 0.11 -1.6 306,165 + chpX Synpcc7942_0308 0.17 -0.6 306,186 + chpX Synpcc7942_0308 0.19 +0.4 306,227 + chpX Synpcc7942_0308 0.23 -1.0 306,227 + chpX Synpcc7942_0308 0.23 -0.0 306,264 + chpX Synpcc7942_0308 0.26 +0.4 306,342 + chpX Synpcc7942_0308 0.33 -0.8 306,352 - chpX Synpcc7942_0308 0.34 -2.7 306,377 + chpX Synpcc7942_0308 0.36 +1.0 306,398 + chpX Synpcc7942_0308 0.38 -0.6 306,398 + chpX Synpcc7942_0308 0.38 +0.2 306,400 - chpX Synpcc7942_0308 0.38 -0.6 306,412 + chpX Synpcc7942_0308 0.39 +0.5 306,597 - chpX Synpcc7942_0308 0.55 +0.4 306,606 + chpX Synpcc7942_0308 0.56 -1.5 306,616 + chpX Synpcc7942_0308 0.57 -1.8 306,629 + chpX Synpcc7942_0308 0.58 -0.6 306,637 - chpX Synpcc7942_0308 0.59 -1.2 306,637 - chpX Synpcc7942_0308 0.59 -0.0 306,675 + chpX Synpcc7942_0308 0.62 +1.0 306,687 + chpX Synpcc7942_0308 0.63 +1.0 306,691 + chpX Synpcc7942_0308 0.63 +1.0 306,748 + chpX Synpcc7942_0308 0.68 -1.8 306,804 + chpX Synpcc7942_0308 0.73 -0.0 306,819 - chpX Synpcc7942_0308 0.75 -0.0 306,832 - chpX Synpcc7942_0308 0.76 -0.8 306,862 + -0.3 306,941 - chpX Synpcc7942_0308 0.85 -0.2 306,959 + chpX Synpcc7942_0308 0.87 -0.6 306,970 + chpX Synpcc7942_0308 0.88 -0.0 306,972 - chpX Synpcc7942_0308 0.88 -0.4 306,978 - chpX Synpcc7942_0308 0.89 -0.0 306,989 + chpX Synpcc7942_0308 0.89 -1.0 306,994 - chpX Synpcc7942_0308 0.90 -1.6 306,994 - chpX Synpcc7942_0308 0.90 +1.0 307,017 + +1.0 307,035 - -2.8 307,084 - -0.6 307,085 + -0.8 307,087 + -0.7 307,088 - -2.2 307,095 - -0.0 307,139 - -0.3 307,169 - +2.0 307,169 - +0.4 307,195 + -1.4 307,209 - -0.9 307,209 - -1.0 307,213 + -0.0 307,216 - +0.2 307,286 - -1.0 307,286 - +0.2 307,296 + -0.0 307,323 + +1.6 307,331 - ndhF4 Synpcc7942_0309 0.10 -0.6 307,331 - ndhF4 Synpcc7942_0309 0.10 -1.4 307,356 + ndhF4 Synpcc7942_0309 0.11 -0.7 307,356 + ndhF4 Synpcc7942_0309 0.11 +1.0 307,356 + ndhF4 Synpcc7942_0309 0.11 -1.6 307,356 + ndhF4 Synpcc7942_0309 0.11 +1.0 307,364 - ndhF4 Synpcc7942_0309 0.12 +1.0 307,364 - ndhF4 Synpcc7942_0309 0.12 -1.1 307,384 - ndhF4 Synpcc7942_0309 0.13 -1.1 307,385 + ndhF4 Synpcc7942_0309 0.13 +0.5 307,413 - ndhF4 Synpcc7942_0309 0.14 -3.2 307,453 - +1.0 307,453 - ndhF4 Synpcc7942_0309 0.16 +1.0 307,455 + ndhF4 Synpcc7942_0309 0.17 -1.4 307,455 + ndhF4 Synpcc7942_0309 0.17 -0.0 307,506 - ndhF4 Synpcc7942_0309 0.19 -2.2 307,512 - ndhF4 Synpcc7942_0309 0.20 -1.1 307,583 + ndhF4 Synpcc7942_0309 0.23 +0.1 307,596 - ndhF4 Synpcc7942_0309 0.24 -1.0 307,680 + ndhF4 Synpcc7942_0309 0.28 -1.2 307,688 + ndhF4 Synpcc7942_0309 0.29 -0.5 307,688 - ndhF4 Synpcc7942_0309 0.29 -1.0 307,690 + ndhF4 Synpcc7942_0309 0.29 +1.2 307,718 + +1.0 307,726 - ndhF4 Synpcc7942_0309 0.31 -0.0 307,804 + ndhF4 Synpcc7942_0309 0.35 -2.4 307,878 + ndhF4 Synpcc7942_0309 0.39 -1.8 307,881 - ndhF4 Synpcc7942_0309 0.39 +0.4 307,888 - ndhF4 Synpcc7942_0309 0.39 +1.1 307,927 + ndhF4 Synpcc7942_0309 0.41 +1.0 308,002 + ndhF4 Synpcc7942_0309 0.45 +1.0 308,004 + ndhF4 Synpcc7942_0309 0.45 -1.3 308,004 + ndhF4 Synpcc7942_0309 0.45 -2.0 308,012 - ndhF4 Synpcc7942_0309 0.46 +1.0 308,028 + ndhF4 Synpcc7942_0309 0.47 -0.5 308,038 + ndhF4 Synpcc7942_0309 0.47 +2.0 308,046 - ndhF4 Synpcc7942_0309 0.48 -0.7 308,046 - ndhF4 Synpcc7942_0309 0.48 -1.7 308,067 + ndhF4 Synpcc7942_0309 0.49 -1.5 308,067 - ndhF4 Synpcc7942_0309 0.49 -0.2 308,099 - ndhF4 Synpcc7942_0309 0.50 +0.2 308,100 - ndhF4 Synpcc7942_0309 0.50 +2.2
Or see this region's nucleotide sequence