Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0102 and Synpcc7942_0103 are separated by 30 nucleotides Synpcc7942_0103 and Synpcc7942_0104 overlap by 14 nucleotides Synpcc7942_0104 and nadE are separated by 244 nucleotides
Synpcc7942_0102: Synpcc7942_0102 - hypothetical protein, at 102,674 to 103,237
_0102
Synpcc7942_0103: Synpcc7942_0103 - Ankyrin, at 103,268 to 103,645
_0103
Synpcc7942_0104: Synpcc7942_0104 - hypothetical protein, at 103,632 to 104,408
_0104
Synpcc7942_0105: nadE - NAD synthetase, at 104,653 to 106,377
nadE
Position (kb) 103
104
105 Strain fitness (log2 ratio) -2
-1
0
1
2 at 102.635 kb on + strand at 102.635 kb on + strand at 102.680 kb on - strand at 102.809 kb on + strand, within Synpcc7942_0102 at 102.875 kb on + strand, within Synpcc7942_0102 at 102.877 kb on + strand, within Synpcc7942_0102 at 102.943 kb on - strand, within Synpcc7942_0102 at 102.948 kb on - strand, within Synpcc7942_0102 at 102.986 kb on - strand, within Synpcc7942_0102 at 103.248 kb on - strand at 103.293 kb on + strand at 103.301 kb on - strand at 103.307 kb on + strand, within Synpcc7942_0103 at 103.438 kb on - strand, within Synpcc7942_0103 at 103.508 kb on + strand, within Synpcc7942_0103 at 103.620 kb on - strand at 103.700 kb on - strand at 103.757 kb on - strand, within Synpcc7942_0104 at 103.757 kb on - strand, within Synpcc7942_0104 at 103.907 kb on - strand, within Synpcc7942_0104 at 103.947 kb on + strand, within Synpcc7942_0104 at 103.957 kb on + strand, within Synpcc7942_0104 at 103.957 kb on + strand, within Synpcc7942_0104 at 103.957 kb on + strand, within Synpcc7942_0104 at 103.989 kb on - strand, within Synpcc7942_0104 at 103.992 kb on + strand, within Synpcc7942_0104 at 104.047 kb on + strand, within Synpcc7942_0104 at 104.117 kb on + strand, within Synpcc7942_0104 at 104.137 kb on + strand, within Synpcc7942_0104 at 104.148 kb on - strand, within Synpcc7942_0104 at 104.188 kb on + strand, within Synpcc7942_0104 at 104.188 kb on + strand, within Synpcc7942_0104 at 104.196 kb on - strand, within Synpcc7942_0104 at 104.196 kb on - strand, within Synpcc7942_0104 at 104.239 kb on + strand, within Synpcc7942_0104 at 104.247 kb on + strand, within Synpcc7942_0104 at 104.258 kb on - strand, within Synpcc7942_0104 at 104.280 kb on + strand, within Synpcc7942_0104 at 104.287 kb on + strand, within Synpcc7942_0104 at 104.288 kb on - strand, within Synpcc7942_0104 at 104.294 kb on + strand, within Synpcc7942_0104 at 104.299 kb on + strand, within Synpcc7942_0104 at 104.302 kb on - strand, within Synpcc7942_0104 at 104.319 kb on - strand, within Synpcc7942_0104 at 104.331 kb on + strand at 104.339 kb on - strand at 104.346 kb on + strand at 104.364 kb on - strand at 104.365 kb on - strand at 104.380 kb on + strand at 104.574 kb on + strand at 104.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 102,635 + -0.6 102,635 + -0.6 102,680 - +0.3 102,809 + Synpcc7942_0102 0.24 +0.8 102,875 + Synpcc7942_0102 0.36 -1.0 102,877 + Synpcc7942_0102 0.36 +1.6 102,943 - Synpcc7942_0102 0.48 -0.5 102,948 - Synpcc7942_0102 0.49 -1.3 102,986 - Synpcc7942_0102 0.55 -0.6 103,248 - -0.6 103,293 + +0.4 103,301 - +0.3 103,307 + Synpcc7942_0103 0.10 -1.9 103,438 - Synpcc7942_0103 0.45 -0.3 103,508 + Synpcc7942_0103 0.63 -2.1 103,620 - -0.9 103,700 - -0.6 103,757 - Synpcc7942_0104 0.16 -1.5 103,757 - Synpcc7942_0104 0.16 -1.4 103,907 - Synpcc7942_0104 0.35 -1.3 103,947 + Synpcc7942_0104 0.41 -1.0 103,957 + Synpcc7942_0104 0.42 -2.3 103,957 + Synpcc7942_0104 0.42 -0.6 103,957 + Synpcc7942_0104 0.42 +0.8 103,989 - Synpcc7942_0104 0.46 -1.9 103,992 + Synpcc7942_0104 0.46 -1.6 104,047 + Synpcc7942_0104 0.53 +1.0 104,117 + Synpcc7942_0104 0.62 +1.0 104,137 + Synpcc7942_0104 0.65 -0.0 104,148 - Synpcc7942_0104 0.66 +1.0 104,188 + Synpcc7942_0104 0.72 -1.3 104,188 + Synpcc7942_0104 0.72 -2.2 104,196 - Synpcc7942_0104 0.73 -0.0 104,196 - Synpcc7942_0104 0.73 +1.2 104,239 + Synpcc7942_0104 0.78 -0.3 104,247 + Synpcc7942_0104 0.79 -1.3 104,258 - Synpcc7942_0104 0.81 -0.6 104,280 + Synpcc7942_0104 0.83 -1.1 104,287 + Synpcc7942_0104 0.84 -0.6 104,288 - Synpcc7942_0104 0.84 -1.1 104,294 + Synpcc7942_0104 0.85 +1.8 104,299 + Synpcc7942_0104 0.86 -0.2 104,302 - Synpcc7942_0104 0.86 +2.0 104,319 - Synpcc7942_0104 0.88 -1.3 104,331 + -1.5 104,339 - +1.7 104,346 + +0.6 104,364 - -1.5 104,365 - +1.6 104,380 + +1.0 104,574 + -1.4 104,581 - -1.8
Or see this region's nucleotide sequence