Experiment: Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0041 and rpmB are separated by 109 nucleotides rpmB and Synpcc7942_0043 are separated by 39 nucleotides Synpcc7942_0043 and Synpcc7942_0044 overlap by 2 nucleotides
Synpcc7942_0041: Synpcc7942_0041 - probable transport protein, at 39,441 to 40,745
_0041
Synpcc7942_0042: rpmB - 50S ribosomal protein L28, at 40,855 to 41,091
rpmB
Synpcc7942_0043: Synpcc7942_0043 - hypothetical protein, at 41,131 to 41,496
_0043
Synpcc7942_0044: Synpcc7942_0044 - hypothetical protein, at 41,495 to 43,477
_0044
Position (kb) 41
42 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 40.132 kb on + strand, within Synpcc7942_0041 at 40.137 kb on + strand, within Synpcc7942_0041 at 40.145 kb on - strand, within Synpcc7942_0041 at 40.157 kb on + strand at 40.158 kb on + strand, within Synpcc7942_0041 at 40.177 kb on + strand, within Synpcc7942_0041 at 40.185 kb on - strand, within Synpcc7942_0041 at 40.185 kb on - strand, within Synpcc7942_0041 at 40.194 kb on - strand, within Synpcc7942_0041 at 40.249 kb on - strand, within Synpcc7942_0041 at 40.254 kb on + strand, within Synpcc7942_0041 at 40.276 kb on + strand at 40.281 kb on + strand, within Synpcc7942_0041 at 40.281 kb on + strand, within Synpcc7942_0041 at 40.286 kb on + strand, within Synpcc7942_0041 at 40.289 kb on - strand, within Synpcc7942_0041 at 40.289 kb on - strand, within Synpcc7942_0041 at 40.313 kb on + strand, within Synpcc7942_0041 at 40.321 kb on - strand, within Synpcc7942_0041 at 40.323 kb on + strand, within Synpcc7942_0041 at 40.330 kb on + strand, within Synpcc7942_0041 at 40.336 kb on + strand, within Synpcc7942_0041 at 40.336 kb on + strand, within Synpcc7942_0041 at 40.344 kb on - strand, within Synpcc7942_0041 at 40.344 kb on - strand, within Synpcc7942_0041 at 40.359 kb on + strand, within Synpcc7942_0041 at 40.368 kb on + strand, within Synpcc7942_0041 at 40.397 kb on - strand, within Synpcc7942_0041 at 40.406 kb on - strand, within Synpcc7942_0041 at 40.415 kb on + strand, within Synpcc7942_0041 at 40.423 kb on - strand, within Synpcc7942_0041 at 40.447 kb on + strand, within Synpcc7942_0041 at 40.490 kb on + strand, within Synpcc7942_0041 at 40.494 kb on - strand, within Synpcc7942_0041 at 40.516 kb on + strand, within Synpcc7942_0041 at 40.538 kb on + strand, within Synpcc7942_0041 at 40.541 kb on - strand, within Synpcc7942_0041 at 40.541 kb on - strand, within Synpcc7942_0041 at 40.546 kb on - strand, within Synpcc7942_0041 at 40.547 kb on + strand, within Synpcc7942_0041 at 40.550 kb on + strand, within Synpcc7942_0041 at 40.552 kb on + strand, within Synpcc7942_0041 at 40.558 kb on - strand, within Synpcc7942_0041 at 40.573 kb on - strand, within Synpcc7942_0041 at 40.583 kb on + strand, within Synpcc7942_0041 at 40.596 kb on - strand, within Synpcc7942_0041 at 40.596 kb on - strand, within Synpcc7942_0041 at 40.632 kb on - strand at 40.651 kb on + strand at 40.676 kb on + strand at 40.676 kb on + strand at 40.676 kb on + strand at 40.676 kb on + strand at 40.684 kb on - strand at 40.684 kb on - strand at 40.719 kb on + strand at 40.719 kb on - strand at 40.721 kb on + strand at 40.721 kb on + strand at 40.721 kb on + strand at 40.721 kb on + strand at 40.727 kb on - strand at 40.729 kb on - strand at 40.729 kb on - strand at 40.729 kb on - strand at 40.729 kb on - strand at 40.745 kb on + strand at 40.754 kb on - strand at 40.805 kb on + strand at 40.810 kb on + strand at 40.815 kb on + strand at 40.818 kb on - strand at 40.823 kb on - strand at 40.843 kb on - strand at 40.844 kb on - strand at 40.853 kb on + strand at 40.861 kb on - strand at 40.904 kb on + strand, within rpmB at 40.917 kb on - strand, within rpmB at 41.041 kb on + strand, within rpmB at 41.042 kb on + strand, within rpmB at 41.050 kb on - strand, within rpmB at 41.130 kb on + strand at 41.138 kb on + strand at 41.138 kb on - strand at 41.176 kb on + strand, within Synpcc7942_0043 at 41.192 kb on - strand, within Synpcc7942_0043 at 41.194 kb on + strand, within Synpcc7942_0043 at 41.212 kb on + strand, within Synpcc7942_0043 at 41.212 kb on + strand at 41.220 kb on - strand, within Synpcc7942_0043 at 41.256 kb on - strand, within Synpcc7942_0043 at 41.264 kb on - strand, within Synpcc7942_0043 at 41.277 kb on - strand, within Synpcc7942_0043 at 41.297 kb on - strand, within Synpcc7942_0043 at 41.321 kb on - strand, within Synpcc7942_0043 at 41.339 kb on + strand, within Synpcc7942_0043 at 41.339 kb on + strand, within Synpcc7942_0043 at 41.347 kb on - strand, within Synpcc7942_0043 at 41.372 kb on + strand, within Synpcc7942_0043 at 41.391 kb on - strand, within Synpcc7942_0043 at 41.415 kb on - strand, within Synpcc7942_0043 at 41.420 kb on - strand, within Synpcc7942_0043 at 41.440 kb on + strand, within Synpcc7942_0043 at 41.492 kb on + strand at 41.492 kb on + strand at 41.492 kb on + strand at 41.492 kb on + strand at 41.492 kb on + strand at 41.495 kb on - strand at 41.499 kb on + strand at 41.500 kb on - strand at 41.500 kb on - strand at 41.500 kb on - strand at 41.543 kb on + strand at 41.544 kb on - strand at 41.558 kb on - strand at 41.558 kb on - strand at 41.579 kb on - strand at 41.579 kb on - strand at 41.581 kb on + strand at 41.581 kb on + strand at 41.581 kb on - strand at 41.585 kb on + strand at 41.585 kb on + strand at 41.585 kb on + strand at 41.585 kb on + strand at 41.585 kb on + strand at 41.589 kb on - strand at 41.593 kb on - strand at 41.593 kb on - strand at 41.593 kb on - strand at 41.593 kb on - strand at 41.593 kb on - strand at 41.643 kb on + strand at 41.651 kb on - strand at 41.659 kb on - strand at 41.675 kb on - strand at 41.706 kb on - strand, within Synpcc7942_0044 at 41.710 kb on - strand, within Synpcc7942_0044 at 41.712 kb on + strand, within Synpcc7942_0044 at 41.717 kb on - strand, within Synpcc7942_0044 at 41.719 kb on + strand, within Synpcc7942_0044 at 41.719 kb on + strand, within Synpcc7942_0044 at 41.732 kb on - strand, within Synpcc7942_0044 at 41.732 kb on - strand, within Synpcc7942_0044 at 41.734 kb on + strand, within Synpcc7942_0044 at 41.734 kb on + strand, within Synpcc7942_0044 at 41.734 kb on + strand, within Synpcc7942_0044 at 41.742 kb on - strand, within Synpcc7942_0044 at 41.745 kb on + strand, within Synpcc7942_0044 at 41.785 kb on - strand, within Synpcc7942_0044 at 41.817 kb on - strand, within Synpcc7942_0044 at 41.826 kb on + strand, within Synpcc7942_0044 at 41.856 kb on + strand, within Synpcc7942_0044 at 41.891 kb on + strand, within Synpcc7942_0044 at 41.926 kb on - strand, within Synpcc7942_0044 at 41.959 kb on - strand, within Synpcc7942_0044 at 41.961 kb on - strand, within Synpcc7942_0044 at 41.988 kb on + strand, within Synpcc7942_0044 at 42.040 kb on + strand, within Synpcc7942_0044 at 42.040 kb on + strand at 42.040 kb on + strand, within Synpcc7942_0044 at 42.040 kb on + strand, within Synpcc7942_0044 at 42.040 kb on + strand, within Synpcc7942_0044 at 42.042 kb on + strand, within Synpcc7942_0044 at 42.042 kb on + strand, within Synpcc7942_0044 at 42.048 kb on - strand, within Synpcc7942_0044 at 42.048 kb on - strand, within Synpcc7942_0044 at 42.048 kb on - strand, within Synpcc7942_0044 at 42.048 kb on - strand, within Synpcc7942_0044 at 42.048 kb on - strand, within Synpcc7942_0044 at 42.048 kb on - strand, within Synpcc7942_0044 at 42.052 kb on + strand, within Synpcc7942_0044 at 42.151 kb on + strand, within Synpcc7942_0044 at 42.196 kb on - strand, within Synpcc7942_0044 at 42.196 kb on - strand, within Synpcc7942_0044 at 42.230 kb on - strand, within Synpcc7942_0044 at 42.277 kb on + strand at 42.277 kb on + strand, within Synpcc7942_0044 at 42.284 kb on + strand, within Synpcc7942_0044 at 42.292 kb on - strand, within Synpcc7942_0044 at 42.322 kb on + strand, within Synpcc7942_0044 at 42.323 kb on + strand, within Synpcc7942_0044 at 42.350 kb on + strand, within Synpcc7942_0044 at 42.371 kb on + strand, within Synpcc7942_0044 at 42.371 kb on + strand, within Synpcc7942_0044 at 42.371 kb on + strand, within Synpcc7942_0044 at 42.371 kb on + strand, within Synpcc7942_0044 at 42.372 kb on - strand, within Synpcc7942_0044 at 42.379 kb on - strand, within Synpcc7942_0044 at 42.389 kb on - strand, within Synpcc7942_0044 at 42.406 kb on + strand, within Synpcc7942_0044 at 42.414 kb on - strand, within Synpcc7942_0044 at 42.416 kb on + strand, within Synpcc7942_0044 at 42.416 kb on + strand, within Synpcc7942_0044 at 42.416 kb on + strand, within Synpcc7942_0044 at 42.418 kb on + strand, within Synpcc7942_0044 at 42.424 kb on - strand, within Synpcc7942_0044 at 42.424 kb on - strand, within Synpcc7942_0044 at 42.441 kb on + strand, within Synpcc7942_0044 at 42.443 kb on + strand, within Synpcc7942_0044 at 42.443 kb on + strand at 42.451 kb on - strand, within Synpcc7942_0044 at 42.451 kb on - strand, within Synpcc7942_0044 at 42.463 kb on + strand, within Synpcc7942_0044 at 42.486 kb on + strand, within Synpcc7942_0044 at 42.491 kb on + strand, within Synpcc7942_0044
Per-strain Table
Position Strand Gene LocusTag Fraction Biofilm Wash/Settlers in WT Conditioned Media in Test Tubes with Spin rep 1 remove 40,132 + Synpcc7942_0041 0.53 +1.0 40,137 + Synpcc7942_0041 0.53 +1.0 40,145 - Synpcc7942_0041 0.54 -1.1 40,157 + +0.4 40,158 + Synpcc7942_0041 0.55 +0.2 40,177 + Synpcc7942_0041 0.56 -0.8 40,185 - Synpcc7942_0041 0.57 -0.2 40,185 - Synpcc7942_0041 0.57 -0.0 40,194 - Synpcc7942_0041 0.58 +0.6 40,249 - Synpcc7942_0041 0.62 -0.8 40,254 + Synpcc7942_0041 0.62 -0.9 40,276 + -0.9 40,281 + Synpcc7942_0041 0.64 -0.0 40,281 + Synpcc7942_0041 0.64 -0.3 40,286 + Synpcc7942_0041 0.65 -2.2 40,289 - Synpcc7942_0041 0.65 +0.4 40,289 - Synpcc7942_0041 0.65 +0.6 40,313 + Synpcc7942_0041 0.67 -1.0 40,321 - Synpcc7942_0041 0.67 +2.0 40,323 + Synpcc7942_0041 0.68 -1.2 40,330 + Synpcc7942_0041 0.68 -1.1 40,336 + Synpcc7942_0041 0.69 -0.0 40,336 + Synpcc7942_0041 0.69 -0.5 40,344 - Synpcc7942_0041 0.69 -1.1 40,344 - Synpcc7942_0041 0.69 +2.6 40,359 + Synpcc7942_0041 0.70 -0.4 40,368 + Synpcc7942_0041 0.71 +0.6 40,397 - Synpcc7942_0041 0.73 +1.0 40,406 - Synpcc7942_0041 0.74 +1.0 40,415 + Synpcc7942_0041 0.75 +1.0 40,423 - Synpcc7942_0041 0.75 +0.6 40,447 + Synpcc7942_0041 0.77 -0.5 40,490 + Synpcc7942_0041 0.80 -1.7 40,494 - Synpcc7942_0041 0.81 -1.1 40,516 + Synpcc7942_0041 0.82 +0.7 40,538 + Synpcc7942_0041 0.84 +1.0 40,541 - Synpcc7942_0041 0.84 -0.9 40,541 - Synpcc7942_0041 0.84 +1.0 40,546 - Synpcc7942_0041 0.85 +2.0 40,547 + Synpcc7942_0041 0.85 +1.0 40,550 + Synpcc7942_0041 0.85 +1.4 40,552 + Synpcc7942_0041 0.85 -0.7 40,558 - Synpcc7942_0041 0.86 -0.8 40,573 - Synpcc7942_0041 0.87 -1.1 40,583 + Synpcc7942_0041 0.88 -0.5 40,596 - Synpcc7942_0041 0.89 -0.8 40,596 - Synpcc7942_0041 0.89 +2.0 40,632 - +2.0 40,651 + -1.8 40,676 + -0.9 40,676 + -2.5 40,676 + -1.4 40,676 + -0.3 40,684 - +2.0 40,684 - -0.5 40,719 + -0.3 40,719 - -0.5 40,721 + -0.8 40,721 + -0.7 40,721 + +1.0 40,721 + -0.6 40,727 - +0.8 40,729 - -0.7 40,729 - -1.0 40,729 - +2.1 40,729 - -0.4 40,745 + -0.4 40,754 - -0.6 40,805 + -0.8 40,810 + -0.0 40,815 + -1.0 40,818 - -0.7 40,823 - -0.6 40,843 - -0.0 40,844 - +1.4 40,853 + -0.9 40,861 - +1.0 40,904 + rpmB Synpcc7942_0042 0.21 -1.0 40,917 - rpmB Synpcc7942_0042 0.26 +1.0 41,041 + rpmB Synpcc7942_0042 0.78 -0.7 41,042 + rpmB Synpcc7942_0042 0.79 +1.0 41,050 - rpmB Synpcc7942_0042 0.82 -0.7 41,130 + -0.3 41,138 + +0.6 41,138 - +0.5 41,176 + Synpcc7942_0043 0.12 -2.0 41,192 - Synpcc7942_0043 0.17 +0.7 41,194 + Synpcc7942_0043 0.17 -1.4 41,212 + Synpcc7942_0043 0.22 -1.0 41,212 + -2.3 41,220 - Synpcc7942_0043 0.24 -1.2 41,256 - Synpcc7942_0043 0.34 -2.5 41,264 - Synpcc7942_0043 0.36 -0.0 41,277 - Synpcc7942_0043 0.40 -0.5 41,297 - Synpcc7942_0043 0.45 -0.2 41,321 - Synpcc7942_0043 0.52 +0.4 41,339 + Synpcc7942_0043 0.57 -0.0 41,339 + Synpcc7942_0043 0.57 -0.9 41,347 - Synpcc7942_0043 0.59 -0.6 41,372 + Synpcc7942_0043 0.66 -1.8 41,391 - Synpcc7942_0043 0.71 +2.8 41,415 - Synpcc7942_0043 0.78 -1.0 41,420 - Synpcc7942_0043 0.79 -1.4 41,440 + Synpcc7942_0043 0.84 -1.3 41,492 + -0.2 41,492 + +3.3 41,492 + +1.0 41,492 + -1.5 41,492 + +1.0 41,495 - +0.0 41,499 + -0.9 41,500 - -0.8 41,500 - +1.0 41,500 - +1.4 41,543 + +1.8 41,544 - -1.2 41,558 - -1.3 41,558 - -1.2 41,579 - -1.0 41,579 - -1.1 41,581 + +1.0 41,581 + -1.0 41,581 - +1.0 41,585 + -0.5 41,585 + +1.6 41,585 + +0.4 41,585 + -0.8 41,585 + +2.6 41,589 - +0.1 41,593 - +1.0 41,593 - -0.0 41,593 - +1.0 41,593 - -0.1 41,593 - +2.4 41,643 + +1.0 41,651 - +2.0 41,659 - -2.3 41,675 - -0.8 41,706 - Synpcc7942_0044 0.11 -1.1 41,710 - Synpcc7942_0044 0.11 -1.1 41,712 + Synpcc7942_0044 0.11 +1.0 41,717 - Synpcc7942_0044 0.11 -0.0 41,719 + Synpcc7942_0044 0.11 -0.3 41,719 + Synpcc7942_0044 0.11 -2.3 41,732 - Synpcc7942_0044 0.12 -0.2 41,732 - Synpcc7942_0044 0.12 -0.4 41,734 + Synpcc7942_0044 0.12 -0.9 41,734 + Synpcc7942_0044 0.12 -1.6 41,734 + Synpcc7942_0044 0.12 -0.0 41,742 - Synpcc7942_0044 0.12 -1.3 41,745 + Synpcc7942_0044 0.13 -1.6 41,785 - Synpcc7942_0044 0.15 -1.7 41,817 - Synpcc7942_0044 0.16 -1.1 41,826 + Synpcc7942_0044 0.17 -0.9 41,856 + Synpcc7942_0044 0.18 -0.3 41,891 + Synpcc7942_0044 0.20 +1.0 41,926 - Synpcc7942_0044 0.22 -0.5 41,959 - Synpcc7942_0044 0.23 +1.0 41,961 - Synpcc7942_0044 0.23 +0.4 41,988 + Synpcc7942_0044 0.25 +1.0 42,040 + Synpcc7942_0044 0.27 -1.1 42,040 + -0.0 42,040 + Synpcc7942_0044 0.27 -0.9 42,040 + Synpcc7942_0044 0.27 -0.9 42,040 + Synpcc7942_0044 0.27 -0.5 42,042 + Synpcc7942_0044 0.28 -0.3 42,042 + Synpcc7942_0044 0.28 -1.6 42,048 - Synpcc7942_0044 0.28 +2.6 42,048 - Synpcc7942_0044 0.28 +1.0 42,048 - Synpcc7942_0044 0.28 -1.7 42,048 - Synpcc7942_0044 0.28 +1.0 42,048 - Synpcc7942_0044 0.28 -0.9 42,048 - Synpcc7942_0044 0.28 -1.1 42,052 + Synpcc7942_0044 0.28 +0.6 42,151 + Synpcc7942_0044 0.33 -0.2 42,196 - Synpcc7942_0044 0.35 -1.2 42,196 - Synpcc7942_0044 0.35 +0.7 42,230 - Synpcc7942_0044 0.37 -0.9 42,277 + -1.0 42,277 + Synpcc7942_0044 0.39 +1.0 42,284 + Synpcc7942_0044 0.40 -1.3 42,292 - Synpcc7942_0044 0.40 -0.3 42,322 + Synpcc7942_0044 0.42 +0.4 42,323 + Synpcc7942_0044 0.42 -0.2 42,350 + Synpcc7942_0044 0.43 -1.6 42,371 + Synpcc7942_0044 0.44 -0.5 42,371 + Synpcc7942_0044 0.44 -0.6 42,371 + Synpcc7942_0044 0.44 -0.0 42,371 + Synpcc7942_0044 0.44 +0.5 42,372 - Synpcc7942_0044 0.44 +0.5 42,379 - Synpcc7942_0044 0.45 -0.0 42,389 - Synpcc7942_0044 0.45 -0.4 42,406 + Synpcc7942_0044 0.46 -0.4 42,414 - Synpcc7942_0044 0.46 +0.7 42,416 + Synpcc7942_0044 0.46 -0.9 42,416 + Synpcc7942_0044 0.46 -0.6 42,416 + Synpcc7942_0044 0.46 -1.3 42,418 + Synpcc7942_0044 0.47 -0.9 42,424 - Synpcc7942_0044 0.47 -0.0 42,424 - Synpcc7942_0044 0.47 +2.0 42,441 + Synpcc7942_0044 0.48 +0.4 42,443 + Synpcc7942_0044 0.48 +1.5 42,443 + -1.1 42,451 - Synpcc7942_0044 0.48 -0.8 42,451 - Synpcc7942_0044 0.48 +1.5 42,463 + Synpcc7942_0044 0.49 -1.0 42,486 + Synpcc7942_0044 0.50 +0.6 42,491 + Synpcc7942_0044 0.50 -0.0
Or see this region's nucleotide sequence