Experiment: H2 McCas with 40 minute oxygen exposure
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00800 and MMP_RS00805 are separated by 48 nucleotides MMP_RS00805 and MMP_RS00810 are separated by 45 nucleotides MMP_RS00810 and MMP_RS00815 are separated by 29 nucleotides
MMP_RS00800: MMP_RS00800 - Coenzyme F420 hydrogenase/dehydrogenase beta subunit C-terminal domain, at 147,092 to 148,219
_RS00800
MMP_RS00805: MMP_RS00805 - carbamoyltransferase HypF, at 148,268 to 150,583
_RS00805
MMP_RS00810: MMP_RS00810 - topoisomerase DNA-binding C4 zinc finger domain-containing protein, at 150,629 to 151,501
_RS00810
MMP_RS00815: MMP_RS00815 - thiamine pyrophosphate-binding protein, at 151,531 to 153,036
_RS00815
Position (kb) 148
149
150
151 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 147.396 kb on - strand, within MMP_RS00800 at 147.440 kb on + strand, within MMP_RS00800 at 147.440 kb on + strand, within MMP_RS00800 at 147.440 kb on + strand, within MMP_RS00800 at 147.507 kb on + strand, within MMP_RS00800 at 147.528 kb on - strand, within MMP_RS00800 at 147.957 kb on - strand, within MMP_RS00800 at 147.957 kb on - strand, within MMP_RS00800 at 147.957 kb on - strand, within MMP_RS00800 at 147.958 kb on + strand, within MMP_RS00800 at 147.958 kb on + strand, within MMP_RS00800 at 147.966 kb on - strand, within MMP_RS00800 at 147.967 kb on + strand, within MMP_RS00800 at 148.001 kb on - strand, within MMP_RS00800 at 148.021 kb on + strand, within MMP_RS00800 at 148.104 kb on - strand, within MMP_RS00800 at 148.210 kb on + strand at 148.218 kb on + strand at 148.264 kb on - strand at 148.265 kb on + strand at 148.295 kb on + strand at 148.441 kb on - strand at 148.441 kb on - strand at 148.442 kb on + strand at 148.580 kb on - strand at 148.580 kb on - strand at 148.583 kb on - strand at 148.693 kb on + strand at 149.177 kb on - strand at 149.287 kb on + strand at 149.514 kb on - strand at 149.635 kb on + strand at 149.639 kb on - strand at 149.761 kb on - strand at 149.802 kb on - strand at 149.843 kb on + strand at 149.846 kb on + strand at 150.058 kb on - strand at 150.353 kb on - strand at 150.353 kb on - strand at 150.353 kb on - strand at 150.566 kb on - strand at 150.567 kb on + strand at 150.641 kb on - strand at 150.700 kb on - strand at 150.742 kb on - strand, within MMP_RS00810 at 150.812 kb on + strand, within MMP_RS00810 at 150.813 kb on - strand, within MMP_RS00810 at 150.813 kb on - strand, within MMP_RS00810 at 150.813 kb on - strand, within MMP_RS00810 at 150.843 kb on - strand, within MMP_RS00810 at 150.843 kb on - strand, within MMP_RS00810 at 150.847 kb on - strand, within MMP_RS00810 at 150.885 kb on + strand, within MMP_RS00810 at 151.021 kb on + strand, within MMP_RS00810 at 151.105 kb on - strand, within MMP_RS00810 at 151.159 kb on - strand, within MMP_RS00810 at 151.264 kb on + strand, within MMP_RS00810 at 151.277 kb on - strand, within MMP_RS00810 at 151.277 kb on - strand, within MMP_RS00810 at 151.570 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas with 40 minute oxygen exposure remove 147,396 - MMP_RS00800 0.27 +1.1 147,440 + MMP_RS00800 0.31 +0.6 147,440 + MMP_RS00800 0.31 +0.8 147,440 + MMP_RS00800 0.31 +0.0 147,507 + MMP_RS00800 0.37 +0.4 147,528 - MMP_RS00800 0.39 +0.6 147,957 - MMP_RS00800 0.77 +0.3 147,957 - MMP_RS00800 0.77 +1.3 147,957 - MMP_RS00800 0.77 -0.3 147,958 + MMP_RS00800 0.77 +0.7 147,958 + MMP_RS00800 0.77 +0.9 147,966 - MMP_RS00800 0.77 +0.7 147,967 + MMP_RS00800 0.78 +1.0 148,001 - MMP_RS00800 0.81 -0.3 148,021 + MMP_RS00800 0.82 +1.4 148,104 - MMP_RS00800 0.90 +0.8 148,210 + -0.8 148,218 + +1.1 148,264 - -0.2 148,265 + +0.3 148,295 + -1.6 148,441 - -2.9 148,441 - -2.4 148,442 + -2.2 148,580 - -3.6 148,580 - -4.5 148,583 - -4.5 148,693 + -2.9 149,177 - -2.1 149,287 + -4.7 149,514 - -2.2 149,635 + -2.8 149,639 - -3.6 149,761 - -3.1 149,802 - -3.6 149,843 + -2.2 149,846 + -5.0 150,058 - -4.0 150,353 - -2.7 150,353 - -3.2 150,353 - -2.3 150,566 - -3.1 150,567 + -2.4 150,641 - -3.1 150,700 - -2.7 150,742 - MMP_RS00810 0.13 -2.5 150,812 + MMP_RS00810 0.21 -4.8 150,813 - MMP_RS00810 0.21 -2.9 150,813 - MMP_RS00810 0.21 -2.9 150,813 - MMP_RS00810 0.21 -3.4 150,843 - MMP_RS00810 0.25 -3.2 150,843 - MMP_RS00810 0.25 -3.0 150,847 - MMP_RS00810 0.25 -3.1 150,885 + MMP_RS00810 0.29 -2.7 151,021 + MMP_RS00810 0.45 -3.3 151,105 - MMP_RS00810 0.55 -3.6 151,159 - MMP_RS00810 0.61 -3.0 151,264 + MMP_RS00810 0.73 -4.3 151,277 - MMP_RS00810 0.74 -2.7 151,277 - MMP_RS00810 0.74 -3.3 151,570 - -3.9
Or see this region's nucleotide sequence