Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27010 and BWI76_RS27015 overlap by 4 nucleotides BWI76_RS27015 and BWI76_RS27020 are separated by 168 nucleotides BWI76_RS27020 and BWI76_RS27025 are separated by 75 nucleotides
BWI76_RS27010: BWI76_RS27010 - O-acetyltransferase, at 5,603,429 to 5,604,424
_RS27010
BWI76_RS27015: BWI76_RS27015 - hypothetical protein, at 5,604,421 to 5,604,801
_RS27015
BWI76_RS27020: BWI76_RS27020 - Xylulose kinase (EC 2.7.1.17) (from data) , at 5,604,970 to 5,606,424
_RS27020
BWI76_RS27025: BWI76_RS27025 - Xylose isomerase (EC 5.3.1.5) (from data) , at 5,606,500 to 5,607,822
_RS27025
Position (kb)
5604
5605
5606
5607 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5603.990 kb on + strand, within BWI76_RS27010 at 5603.998 kb on - strand, within BWI76_RS27010 at 5604.043 kb on + strand, within BWI76_RS27010 at 5604.053 kb on + strand, within BWI76_RS27010 at 5604.056 kb on - strand, within BWI76_RS27010 at 5604.061 kb on - strand, within BWI76_RS27010 at 5604.061 kb on - strand, within BWI76_RS27010 at 5604.061 kb on - strand, within BWI76_RS27010 at 5604.203 kb on - strand at 5604.210 kb on + strand, within BWI76_RS27010 at 5604.364 kb on - strand at 5604.570 kb on + strand, within BWI76_RS27015 at 5604.594 kb on + strand, within BWI76_RS27015 at 5604.684 kb on + strand, within BWI76_RS27015 at 5604.759 kb on + strand, within BWI76_RS27015 at 5604.814 kb on + strand at 5604.814 kb on + strand at 5604.814 kb on + strand at 5604.814 kb on + strand at 5604.822 kb on - strand at 5604.822 kb on - strand at 5604.822 kb on - strand at 5604.822 kb on - strand at 5604.822 kb on - strand at 5604.893 kb on + strand at 5605.154 kb on + strand, within BWI76_RS27020 at 5605.330 kb on - strand, within BWI76_RS27020 at 5605.402 kb on + strand, within BWI76_RS27020 at 5605.429 kb on - strand, within BWI76_RS27020 at 5605.510 kb on + strand, within BWI76_RS27020 at 5605.518 kb on - strand, within BWI76_RS27020 at 5605.549 kb on + strand, within BWI76_RS27020 at 5605.845 kb on + strand, within BWI76_RS27020 at 5606.189 kb on - strand, within BWI76_RS27020 at 5606.209 kb on + strand, within BWI76_RS27020 at 5606.217 kb on - strand, within BWI76_RS27020 at 5606.217 kb on - strand, within BWI76_RS27020 at 5606.266 kb on + strand, within BWI76_RS27020 at 5606.351 kb on + strand at 5606.357 kb on + strand at 5606.357 kb on + strand at 5606.365 kb on - strand at 5606.391 kb on + strand at 5606.399 kb on - strand at 5606.414 kb on - strand at 5606.458 kb on + strand at 5606.458 kb on + strand at 5606.513 kb on - strand at 5606.573 kb on + strand at 5606.573 kb on + strand at 5606.575 kb on - strand at 5606.593 kb on - strand at 5606.608 kb on - strand at 5606.645 kb on - strand, within BWI76_RS27025 at 5606.658 kb on - strand, within BWI76_RS27025 at 5606.767 kb on - strand, within BWI76_RS27025 at 5606.872 kb on + strand, within BWI76_RS27025 at 5606.942 kb on + strand, within BWI76_RS27025 at 5606.960 kb on - strand, within BWI76_RS27025 at 5607.067 kb on + strand, within BWI76_RS27025 at 5607.075 kb on - strand, within BWI76_RS27025 at 5607.131 kb on + strand, within BWI76_RS27025 at 5607.226 kb on + strand, within BWI76_RS27025 at 5607.234 kb on - strand, within BWI76_RS27025 at 5607.401 kb on - strand, within BWI76_RS27025
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 5,603,990 + BWI76_RS27010 0.56 +1.0 5,603,998 - BWI76_RS27010 0.57 +0.0 5,604,043 + BWI76_RS27010 0.62 -0.3 5,604,053 + BWI76_RS27010 0.63 -0.6 5,604,056 - BWI76_RS27010 0.63 -0.7 5,604,061 - BWI76_RS27010 0.63 -0.2 5,604,061 - BWI76_RS27010 0.63 +0.9 5,604,061 - BWI76_RS27010 0.63 -0.3 5,604,203 - -0.1 5,604,210 + BWI76_RS27010 0.78 -0.2 5,604,364 - +0.2 5,604,570 + BWI76_RS27015 0.39 -0.3 5,604,594 + BWI76_RS27015 0.45 +1.2 5,604,684 + BWI76_RS27015 0.69 -0.9 5,604,759 + BWI76_RS27015 0.89 -0.9 5,604,814 + -1.1 5,604,814 + +0.6 5,604,814 + -1.0 5,604,814 + -0.7 5,604,822 - +0.4 5,604,822 - -0.9 5,604,822 - +1.0 5,604,822 - -0.3 5,604,822 - +1.1 5,604,893 + -1.9 5,605,154 + BWI76_RS27020 0.13 -1.1 5,605,330 - BWI76_RS27020 0.25 -0.8 5,605,402 + BWI76_RS27020 0.30 +0.1 5,605,429 - BWI76_RS27020 0.32 +0.9 5,605,510 + BWI76_RS27020 0.37 -0.4 5,605,518 - BWI76_RS27020 0.38 -0.0 5,605,549 + BWI76_RS27020 0.40 -1.3 5,605,845 + BWI76_RS27020 0.60 -2.0 5,606,189 - BWI76_RS27020 0.84 -0.3 5,606,209 + BWI76_RS27020 0.85 -0.1 5,606,217 - BWI76_RS27020 0.86 -0.5 5,606,217 - BWI76_RS27020 0.86 -0.9 5,606,266 + BWI76_RS27020 0.89 -0.3 5,606,351 + -0.1 5,606,357 + +0.2 5,606,357 + +0.2 5,606,365 - -0.3 5,606,391 + +1.8 5,606,399 - -0.1 5,606,414 - -0.2 5,606,458 + -0.4 5,606,458 + -0.9 5,606,513 - -0.1 5,606,573 + -0.8 5,606,573 + -0.2 5,606,575 - -0.3 5,606,593 - -0.9 5,606,608 - -0.5 5,606,645 - BWI76_RS27025 0.11 +0.7 5,606,658 - BWI76_RS27025 0.12 -0.3 5,606,767 - BWI76_RS27025 0.20 +0.9 5,606,872 + BWI76_RS27025 0.28 -1.8 5,606,942 + BWI76_RS27025 0.33 -1.1 5,606,960 - BWI76_RS27025 0.35 -0.2 5,607,067 + BWI76_RS27025 0.43 -0.5 5,607,075 - BWI76_RS27025 0.43 -0.2 5,607,131 + BWI76_RS27025 0.48 -0.2 5,607,226 + BWI76_RS27025 0.55 -0.0 5,607,234 - BWI76_RS27025 0.55 -0.1 5,607,401 - BWI76_RS27025 0.68 +0.0
Or see this region's nucleotide sequence