Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22965 and BWI76_RS22970 overlap by 1 nucleotides BWI76_RS22970 and BWI76_RS22975 are separated by 139 nucleotides BWI76_RS22975 and BWI76_RS22980 are separated by 105 nucleotides
BWI76_RS22965: BWI76_RS22965 - cysteine sulfinate desulfinase, at 4,794,415 to 4,795,620
_RS22965
BWI76_RS22970: BWI76_RS22970 - cysteine desulfurase, sulfur acceptor subunit CsdE, at 4,795,620 to 4,796,060
_RS22970
BWI76_RS22975: BWI76_RS22975 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 4,796,200 to 4,797,009
_RS22975
BWI76_RS22980: BWI76_RS22980 - murein transglycosylase A, at 4,797,115 to 4,798,212
_RS22980
Position (kb) 4796
4797
4798 Strain fitness (log2 ratio) -1
0
1 at 4795.425 kb on + strand, within BWI76_RS22965 at 4795.485 kb on - strand, within BWI76_RS22965 at 4795.627 kb on + strand at 4795.683 kb on - strand, within BWI76_RS22970 at 4796.063 kb on - strand at 4796.075 kb on + strand at 4796.151 kb on + strand at 4796.421 kb on - strand, within BWI76_RS22975 at 4796.438 kb on + strand, within BWI76_RS22975 at 4796.649 kb on - strand, within BWI76_RS22975 at 4796.985 kb on + strand at 4797.213 kb on - strand at 4797.261 kb on + strand, within BWI76_RS22980 at 4797.261 kb on + strand, within BWI76_RS22980 at 4797.269 kb on - strand, within BWI76_RS22980 at 4797.285 kb on + strand, within BWI76_RS22980 at 4797.370 kb on + strand, within BWI76_RS22980 at 4797.424 kb on - strand, within BWI76_RS22980 at 4797.987 kb on - strand, within BWI76_RS22980 at 4797.989 kb on + strand, within BWI76_RS22980
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 4,795,425 + BWI76_RS22965 0.84 +0.3 4,795,485 - BWI76_RS22965 0.89 -0.6 4,795,627 + -0.2 4,795,683 - BWI76_RS22970 0.14 -1.0 4,796,063 - +0.3 4,796,075 + +0.6 4,796,151 + +0.2 4,796,421 - BWI76_RS22975 0.27 -1.1 4,796,438 + BWI76_RS22975 0.29 -1.3 4,796,649 - BWI76_RS22975 0.55 -0.4 4,796,985 + -0.5 4,797,213 - -0.8 4,797,261 + BWI76_RS22980 0.13 -0.4 4,797,261 + BWI76_RS22980 0.13 -0.7 4,797,269 - BWI76_RS22980 0.14 -0.3 4,797,285 + BWI76_RS22980 0.15 -0.5 4,797,370 + BWI76_RS22980 0.23 +0.1 4,797,424 - BWI76_RS22980 0.28 -1.0 4,797,987 - BWI76_RS22980 0.79 -0.2 4,797,989 + BWI76_RS22980 0.80 -1.1
Or see this region's nucleotide sequence