Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19945 and BWI76_RS19950 overlap by 1 nucleotides BWI76_RS19950 and BWI76_RS19955 are separated by 71 nucleotides BWI76_RS19955 and BWI76_RS19960 are separated by 9 nucleotides
BWI76_RS19945: BWI76_RS19945 - ribonucleotide-diphosphate reductase subunit beta, at 4,177,575 to 4,178,705
_RS19945
BWI76_RS19950: BWI76_RS19950 - (2Fe-2S) ferredoxin, at 4,178,705 to 4,178,959
_RS19950
BWI76_RS19955: BWI76_RS19955 - glycerophosphoryl diester phosphodiesterase, at 4,179,031 to 4,180,101
_RS19955
BWI76_RS19960: BWI76_RS19960 - MFS transporter, at 4,180,111 to 4,181,457
_RS19960
Position (kb) 4179
4180
4181 Strain fitness (log2 ratio) -1
0
1
2
3 at 4179.269 kb on - strand, within BWI76_RS19955 at 4179.346 kb on - strand, within BWI76_RS19955 at 4179.401 kb on + strand, within BWI76_RS19955 at 4179.409 kb on - strand, within BWI76_RS19955 at 4179.461 kb on + strand, within BWI76_RS19955 at 4179.562 kb on - strand, within BWI76_RS19955 at 4179.678 kb on - strand, within BWI76_RS19955 at 4180.070 kb on + strand at 4180.636 kb on + strand, within BWI76_RS19960 at 4180.880 kb on + strand, within BWI76_RS19960 at 4180.972 kb on + strand, within BWI76_RS19960 at 4180.980 kb on - strand, within BWI76_RS19960
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 4,179,269 - BWI76_RS19955 0.22 +1.1 4,179,346 - BWI76_RS19955 0.29 +0.9 4,179,401 + BWI76_RS19955 0.35 +1.5 4,179,409 - BWI76_RS19955 0.35 -0.1 4,179,461 + BWI76_RS19955 0.40 +0.1 4,179,562 - BWI76_RS19955 0.50 +3.2 4,179,678 - BWI76_RS19955 0.60 +0.3 4,180,070 + +0.6 4,180,636 + BWI76_RS19960 0.39 -0.1 4,180,880 + BWI76_RS19960 0.57 -0.3 4,180,972 + BWI76_RS19960 0.64 -0.2 4,180,980 - BWI76_RS19960 0.65 -0.5
Or see this region's nucleotide sequence