Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16865 and BWI76_RS16870 are separated by 145 nucleotides BWI76_RS16870 and BWI76_RS16875 are separated by 107 nucleotides BWI76_RS16875 and BWI76_RS16880 are separated by 1 nucleotides
BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579
_RS16865
BWI76_RS16870: BWI76_RS16870 - LysR family transcriptional regulator, at 3,498,725 to 3,499,618
_RS16870
BWI76_RS16875: BWI76_RS16875 - putative type I cysteine dioxygenase, at 3,499,726 to 3,500,322
_RS16875
BWI76_RS16880: BWI76_RS16880 - GlpE family protein, at 3,500,324 to 3,501,904
_RS16880
Position (kb)
3498
3499
3500 Strain fitness (log2 ratio)
-1
0
1 at 3497.738 kb on + strand, within BWI76_RS16865 at 3498.029 kb on + strand, within BWI76_RS16865 at 3498.154 kb on + strand, within BWI76_RS16865 at 3498.173 kb on + strand at 3498.181 kb on - strand, within BWI76_RS16865 at 3498.647 kb on - strand at 3498.975 kb on + strand, within BWI76_RS16870 at 3498.976 kb on - strand, within BWI76_RS16870 at 3499.376 kb on - strand, within BWI76_RS16870 at 3499.812 kb on + strand, within BWI76_RS16875 at 3499.991 kb on + strand, within BWI76_RS16875 at 3500.040 kb on - strand, within BWI76_RS16875 at 3500.097 kb on + strand, within BWI76_RS16875 at 3500.156 kb on + strand, within BWI76_RS16875 at 3500.170 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 3,497,738 + BWI76_RS16865 0.22 -0.1 3,498,029 + BWI76_RS16865 0.49 +0.8 3,498,154 + BWI76_RS16865 0.61 -0.2 3,498,173 + -0.4 3,498,181 - BWI76_RS16865 0.63 -0.1 3,498,647 - +0.3 3,498,975 + BWI76_RS16870 0.28 -1.0 3,498,976 - BWI76_RS16870 0.28 -1.0 3,499,376 - BWI76_RS16870 0.73 -0.2 3,499,812 + BWI76_RS16875 0.14 +1.0 3,499,991 + BWI76_RS16875 0.44 -0.4 3,500,040 - BWI76_RS16875 0.53 -0.2 3,500,097 + BWI76_RS16875 0.62 -0.2 3,500,156 + BWI76_RS16875 0.72 +0.7 3,500,170 - -0.0
Or see this region's nucleotide sequence