Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16260

Experiment: LB in EcoFAB_3.5mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16255 and BWI76_RS16260 are separated by 477 nucleotidesBWI76_RS16260 and BWI76_RS16265 are separated by 74 nucleotides BWI76_RS16255: BWI76_RS16255 - histidine kinase, at 3,370,589 to 3,372,364 _RS16255 BWI76_RS16260: BWI76_RS16260 - two-component sensor histidine kinase, at 3,372,842 to 3,374,143 _RS16260 BWI76_RS16265: BWI76_RS16265 - DNA replication terminus site-binding protein, at 3,374,218 to 3,375,150 _RS16265 BWI76_RS16270: BWI76_RS16270 - fumarate hydratase, class II, at 3,375,140 to 3,376,540 _RS16270 Position (kb) 3372 3373 3374 3375Strain fitness (log2 ratio) -1 0 1at 3371.955 kb on + strand, within BWI76_RS16255at 3372.119 kb on + strand, within BWI76_RS16255at 3372.198 kb on + strandat 3373.360 kb on - strand, within BWI76_RS16260at 3374.038 kb on + strandat 3374.965 kb on + strand, within BWI76_RS16265

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Per-strain Table

Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL
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3,371,955 + BWI76_RS16255 0.77 -0.5
3,372,119 + BWI76_RS16255 0.86 +0.2
3,372,198 + -0.7
3,373,360 - BWI76_RS16260 0.40 -1.1
3,374,038 + -0.3
3,374,965 + BWI76_RS16265 0.80 -0.7

Or see this region's nucleotide sequence