Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13250 and BWI76_RS13255 are separated by 169 nucleotides BWI76_RS13255 and BWI76_RS13260 are separated by 22 nucleotides BWI76_RS13260 and BWI76_RS13265 are separated by 51 nucleotides BWI76_RS13265 and BWI76_RS13270 are separated by 173 nucleotides
BWI76_RS13250: BWI76_RS13250 - cytochrome b561, at 2,763,623 to 2,764,150
_RS13250
BWI76_RS13255: BWI76_RS13255 - hypothetical protein, at 2,764,320 to 2,764,595
_RS13255
BWI76_RS13260: BWI76_RS13260 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 2,764,618 to 2,765,736
_RS13260
BWI76_RS13265: BWI76_RS13265 - hypothetical protein, at 2,765,788 to 2,766,066
_RS13265
BWI76_RS13270: BWI76_RS13270 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 2,766,240 to 2,768,612
_RS13270
Position (kb)
2764
2765
2766 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2763.838 kb on + strand, within BWI76_RS13250 at 2764.748 kb on + strand, within BWI76_RS13260 at 2764.756 kb on - strand, within BWI76_RS13260 at 2764.792 kb on + strand, within BWI76_RS13260 at 2765.157 kb on - strand, within BWI76_RS13260 at 2765.162 kb on - strand, within BWI76_RS13260 at 2765.249 kb on - strand, within BWI76_RS13260 at 2765.256 kb on - strand, within BWI76_RS13260 at 2765.340 kb on + strand, within BWI76_RS13260 at 2765.572 kb on + strand, within BWI76_RS13260 at 2765.654 kb on - strand at 2765.840 kb on - strand, within BWI76_RS13265 at 2765.883 kb on - strand, within BWI76_RS13265 at 2765.883 kb on - strand, within BWI76_RS13265 at 2765.998 kb on + strand, within BWI76_RS13265
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 2,763,838 + BWI76_RS13250 0.41 +0.1 2,764,748 + BWI76_RS13260 0.12 +0.0 2,764,756 - BWI76_RS13260 0.12 +1.1 2,764,792 + BWI76_RS13260 0.16 +1.0 2,765,157 - BWI76_RS13260 0.48 -0.7 2,765,162 - BWI76_RS13260 0.49 -0.3 2,765,249 - BWI76_RS13260 0.56 -0.3 2,765,256 - BWI76_RS13260 0.57 +3.9 2,765,340 + BWI76_RS13260 0.65 -0.1 2,765,572 + BWI76_RS13260 0.85 +0.8 2,765,654 - -0.5 2,765,840 - BWI76_RS13265 0.19 +0.6 2,765,883 - BWI76_RS13265 0.34 -0.6 2,765,883 - BWI76_RS13265 0.34 -0.0 2,765,998 + BWI76_RS13265 0.75 -0.3
Or see this region's nucleotide sequence