Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12910 and BWI76_RS12915 are separated by 17 nucleotides BWI76_RS12915 and BWI76_RS12920 are separated by 51 nucleotides BWI76_RS12920 and BWI76_RS12925 overlap by 4 nucleotides
BWI76_RS12910: BWI76_RS12910 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 2,689,937 to 2,690,743
_RS12910
BWI76_RS12915: BWI76_RS12915 - 4-hydroxyphenylacetate permease, at 2,690,761 to 2,692,134
_RS12915
BWI76_RS12920: BWI76_RS12920 - DNA-binding protein, at 2,692,186 to 2,692,797
_RS12920
BWI76_RS12925: BWI76_RS12925 - efflux transporter periplasmic adaptor subunit, at 2,692,794 to 2,693,717
_RS12925
Position (kb)
2690
2691
2692
2693 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2689.870 kb on + strand at 2689.870 kb on + strand at 2689.870 kb on + strand at 2689.870 kb on + strand at 2689.870 kb on + strand at 2689.878 kb on - strand at 2689.878 kb on - strand at 2689.883 kb on + strand at 2689.911 kb on - strand at 2689.920 kb on + strand at 2689.920 kb on + strand at 2689.928 kb on - strand at 2690.001 kb on + strand at 2690.010 kb on + strand at 2690.017 kb on + strand at 2690.067 kb on + strand, within BWI76_RS12910 at 2690.149 kb on - strand, within BWI76_RS12910 at 2690.251 kb on - strand, within BWI76_RS12910 at 2690.780 kb on - strand at 2690.823 kb on + strand at 2690.978 kb on - strand, within BWI76_RS12915 at 2691.044 kb on - strand, within BWI76_RS12915 at 2691.073 kb on + strand, within BWI76_RS12915 at 2691.073 kb on + strand, within BWI76_RS12915 at 2691.369 kb on + strand, within BWI76_RS12915 at 2691.714 kb on + strand, within BWI76_RS12915 at 2691.751 kb on - strand, within BWI76_RS12915 at 2691.858 kb on + strand, within BWI76_RS12915 at 2691.901 kb on - strand, within BWI76_RS12915 at 2692.037 kb on - strand at 2692.460 kb on - strand, within BWI76_RS12920 at 2692.821 kb on + strand at 2692.824 kb on - strand at 2692.996 kb on - strand, within BWI76_RS12925
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 2,689,870 + -0.4 2,689,870 + -0.2 2,689,870 + -2.1 2,689,870 + -0.4 2,689,870 + -0.0 2,689,878 - +1.7 2,689,878 - -0.8 2,689,883 + +2.7 2,689,911 - -0.1 2,689,920 + -0.7 2,689,920 + -0.4 2,689,928 - -0.1 2,690,001 + -0.3 2,690,010 + +0.3 2,690,017 + -1.1 2,690,067 + BWI76_RS12910 0.16 -2.1 2,690,149 - BWI76_RS12910 0.26 -0.7 2,690,251 - BWI76_RS12910 0.39 -0.3 2,690,780 - +0.4 2,690,823 + -0.4 2,690,978 - BWI76_RS12915 0.16 -0.2 2,691,044 - BWI76_RS12915 0.21 +4.7 2,691,073 + BWI76_RS12915 0.23 -0.1 2,691,073 + BWI76_RS12915 0.23 -0.2 2,691,369 + BWI76_RS12915 0.44 -0.3 2,691,714 + BWI76_RS12915 0.69 +0.2 2,691,751 - BWI76_RS12915 0.72 +1.5 2,691,858 + BWI76_RS12915 0.80 +0.2 2,691,901 - BWI76_RS12915 0.83 +0.4 2,692,037 - -0.2 2,692,460 - BWI76_RS12920 0.45 -0.0 2,692,821 + -0.7 2,692,824 - -0.9 2,692,996 - BWI76_RS12925 0.22 -0.0
Or see this region's nucleotide sequence