Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10830 and BWI76_RS10835 are separated by 50 nucleotides BWI76_RS10835 and BWI76_RS10840 are separated by 155 nucleotides BWI76_RS10840 and BWI76_RS10845 are separated by 122 nucleotides BWI76_RS10845 and BWI76_RS10850 overlap by 4 nucleotides
BWI76_RS10830: BWI76_RS10830 - phosphate starvation-inducible protein PhoH, at 2,288,942 to 2,290,006
_RS10830
BWI76_RS10835: BWI76_RS10835 - hypothetical protein, at 2,290,057 to 2,290,350
_RS10835
BWI76_RS10840: BWI76_RS10840 - membrane protein, at 2,290,506 to 2,291,294
_RS10840
BWI76_RS10845: BWI76_RS10845 - DNA-binding response regulator, at 2,291,417 to 2,292,112
_RS10845
BWI76_RS10850: BWI76_RS10850 - two-component sensor histidine kinase, at 2,292,109 to 2,293,305
_RS10850
Position (kb)
2290
2291
2292 Strain fitness (log2 ratio)
-2
-1
0
1 at 2289.537 kb on - strand, within BWI76_RS10830 at 2289.668 kb on + strand, within BWI76_RS10830 at 2289.708 kb on - strand, within BWI76_RS10830 at 2289.997 kb on + strand at 2290.057 kb on + strand at 2290.065 kb on - strand at 2290.082 kb on - strand at 2290.159 kb on + strand, within BWI76_RS10835 at 2290.566 kb on + strand at 2290.618 kb on - strand, within BWI76_RS10840 at 2290.642 kb on + strand, within BWI76_RS10840 at 2290.642 kb on + strand, within BWI76_RS10840 at 2290.652 kb on + strand, within BWI76_RS10840 at 2290.846 kb on + strand, within BWI76_RS10840 at 2291.271 kb on - strand at 2291.536 kb on + strand, within BWI76_RS10845 at 2291.544 kb on - strand, within BWI76_RS10845 at 2291.617 kb on - strand, within BWI76_RS10845 at 2291.719 kb on + strand, within BWI76_RS10845 at 2291.720 kb on + strand, within BWI76_RS10845 at 2292.001 kb on - strand, within BWI76_RS10845 at 2292.097 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 2,289,537 - BWI76_RS10830 0.56 +0.0 2,289,668 + BWI76_RS10830 0.68 -0.3 2,289,708 - BWI76_RS10830 0.72 -0.3 2,289,997 + -0.4 2,290,057 + +1.2 2,290,065 - +0.4 2,290,082 - -1.0 2,290,159 + BWI76_RS10835 0.35 -0.3 2,290,566 + +0.1 2,290,618 - BWI76_RS10840 0.14 +1.2 2,290,642 + BWI76_RS10840 0.17 +1.2 2,290,642 + BWI76_RS10840 0.17 +0.4 2,290,652 + BWI76_RS10840 0.19 +0.6 2,290,846 + BWI76_RS10840 0.43 -1.2 2,291,271 - +0.6 2,291,536 + BWI76_RS10845 0.17 +0.7 2,291,544 - BWI76_RS10845 0.18 +0.1 2,291,617 - BWI76_RS10845 0.29 -0.6 2,291,719 + BWI76_RS10845 0.43 -0.6 2,291,720 + BWI76_RS10845 0.44 +0.5 2,292,001 - BWI76_RS10845 0.84 -0.6 2,292,097 - -1.8
Or see this region's nucleotide sequence