Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08855 and BWI76_RS08860 are separated by 143 nucleotides BWI76_RS08860 and BWI76_RS08865 overlap by 39 nucleotides BWI76_RS08865 and BWI76_RS08870 are separated by 121 nucleotides
BWI76_RS08855: BWI76_RS08855 - endonuclease/exonuclease/phosphatase, at 1,864,368 to 1,865,117
_RS08855
BWI76_RS08860: BWI76_RS08860 - hypothetical protein, at 1,865,261 to 1,865,671
_RS08860
BWI76_RS08865: BWI76_RS08865 - ABC transporter inner membrane, at 1,865,633 to 1,866,739
_RS08865
BWI76_RS08870: BWI76_RS08870 - inner membrane transport permease YbhS, at 1,866,861 to 1,867,994
_RS08870
Position (kb)
1865
1866
1867 Strain fitness (log2 ratio)
-2
-1
0
1 at 1864.634 kb on + strand, within BWI76_RS08855 at 1865.569 kb on - strand, within BWI76_RS08860 at 1866.259 kb on + strand, within BWI76_RS08865 at 1866.299 kb on - strand, within BWI76_RS08865 at 1866.558 kb on - strand, within BWI76_RS08865 at 1866.639 kb on + strand at 1866.698 kb on + strand at 1866.728 kb on - strand at 1866.775 kb on + strand at 1866.775 kb on + strand at 1866.783 kb on - strand at 1866.790 kb on + strand at 1867.120 kb on + strand, within BWI76_RS08870 at 1867.171 kb on - strand, within BWI76_RS08870 at 1867.263 kb on + strand, within BWI76_RS08870 at 1867.263 kb on - strand, within BWI76_RS08870 at 1867.395 kb on + strand, within BWI76_RS08870 at 1867.403 kb on - strand, within BWI76_RS08870 at 1867.480 kb on - strand, within BWI76_RS08870 at 1867.575 kb on + strand, within BWI76_RS08870
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 1,864,634 + BWI76_RS08855 0.35 -0.4 1,865,569 - BWI76_RS08860 0.75 -0.8 1,866,259 + BWI76_RS08865 0.57 +0.3 1,866,299 - BWI76_RS08865 0.60 +0.3 1,866,558 - BWI76_RS08865 0.84 +1.2 1,866,639 + -1.1 1,866,698 + +0.2 1,866,728 - -0.1 1,866,775 + -0.1 1,866,775 + -1.0 1,866,783 - -0.2 1,866,790 + +0.2 1,867,120 + BWI76_RS08870 0.23 +0.2 1,867,171 - BWI76_RS08870 0.27 +0.2 1,867,263 + BWI76_RS08870 0.35 -0.0 1,867,263 - BWI76_RS08870 0.35 +0.6 1,867,395 + BWI76_RS08870 0.47 -2.7 1,867,403 - BWI76_RS08870 0.48 -0.3 1,867,480 - BWI76_RS08870 0.55 +0.1 1,867,575 + BWI76_RS08870 0.63 +0.4
Or see this region's nucleotide sequence