Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07260 and BWI76_RS07265 are separated by 27 nucleotides BWI76_RS07265 and BWI76_RS07270 overlap by 8 nucleotides BWI76_RS07270 and BWI76_RS07275 overlap by 4 nucleotides
BWI76_RS07260: BWI76_RS07260 - branched chain amino acid ABC transporter substrate-binding protein, at 1,527,758 to 1,528,915
_RS07260
BWI76_RS07265: BWI76_RS07265 - ABC transporter ATP-binding protein, at 1,528,943 to 1,529,662
_RS07265
BWI76_RS07270: BWI76_RS07270 - ABC transporter ATP-binding protein, at 1,529,655 to 1,530,422
_RS07270
BWI76_RS07275: BWI76_RS07275 - ABC transporter, at 1,530,419 to 1,531,525
_RS07275
Position (kb)
1528
1529
1530 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1528.023 kb on + strand, within BWI76_RS07260 at 1528.048 kb on - strand, within BWI76_RS07260 at 1528.503 kb on + strand, within BWI76_RS07260 at 1528.508 kb on + strand, within BWI76_RS07260 at 1528.970 kb on - strand at 1529.017 kb on + strand, within BWI76_RS07265 at 1529.220 kb on - strand, within BWI76_RS07265 at 1529.433 kb on - strand, within BWI76_RS07265 at 1529.539 kb on - strand, within BWI76_RS07265 at 1529.596 kb on - strand at 1529.596 kb on - strand at 1529.890 kb on + strand, within BWI76_RS07270 at 1529.923 kb on - strand, within BWI76_RS07270 at 1530.281 kb on + strand, within BWI76_RS07270 at 1530.289 kb on - strand, within BWI76_RS07270 at 1530.341 kb on + strand, within BWI76_RS07270 at 1530.349 kb on - strand at 1530.447 kb on + strand at 1530.487 kb on + strand at 1530.599 kb on + strand, within BWI76_RS07275
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 1,528,023 + BWI76_RS07260 0.23 +2.4 1,528,048 - BWI76_RS07260 0.25 +0.4 1,528,503 + BWI76_RS07260 0.64 +0.8 1,528,508 + BWI76_RS07260 0.65 +0.3 1,528,970 - -0.0 1,529,017 + BWI76_RS07265 0.10 -0.4 1,529,220 - BWI76_RS07265 0.38 -0.3 1,529,433 - BWI76_RS07265 0.68 -1.6 1,529,539 - BWI76_RS07265 0.83 -1.4 1,529,596 - +0.7 1,529,596 - +0.3 1,529,890 + BWI76_RS07270 0.31 -1.2 1,529,923 - BWI76_RS07270 0.35 +0.1 1,530,281 + BWI76_RS07270 0.82 -0.2 1,530,289 - BWI76_RS07270 0.83 +0.2 1,530,341 + BWI76_RS07270 0.89 -0.1 1,530,349 - +0.5 1,530,447 + -0.5 1,530,487 + +0.1 1,530,599 + BWI76_RS07275 0.16 -1.9
Or see this region's nucleotide sequence