Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03120 and BWI76_RS03125 are separated by 5 nucleotides BWI76_RS03125 and BWI76_RS03130 are separated by 10 nucleotides BWI76_RS03130 and BWI76_RS03135 overlap by 11 nucleotides
BWI76_RS03120: BWI76_RS03120 - ABC transporter ATP-binding protein, at 643,322 to 644,392
_RS03120
BWI76_RS03125: BWI76_RS03125 - phosphodiesterase, at 644,398 to 645,222
_RS03125
BWI76_RS03130: BWI76_RS03130 - putative SN-glycerol-3-phosphate transport system permease, at 645,233 to 646,120
_RS03130
BWI76_RS03135: BWI76_RS03135 - sugar ABC transporter permease, at 646,110 to 646,982
_RS03135
Position (kb)
644
645
646 Strain fitness (log2 ratio)
-1
0
1
2
3 at 643.458 kb on - strand, within BWI76_RS03120 at 643.468 kb on - strand, within BWI76_RS03120 at 643.562 kb on + strand, within BWI76_RS03120 at 643.628 kb on + strand, within BWI76_RS03120 at 643.736 kb on + strand, within BWI76_RS03120 at 643.744 kb on - strand, within BWI76_RS03120 at 643.827 kb on - strand, within BWI76_RS03120 at 644.049 kb on + strand, within BWI76_RS03120 at 644.247 kb on - strand, within BWI76_RS03120 at 644.269 kb on - strand, within BWI76_RS03120 at 644.616 kb on + strand at 644.699 kb on - strand, within BWI76_RS03125 at 644.908 kb on - strand, within BWI76_RS03125 at 644.965 kb on - strand, within BWI76_RS03125 at 645.084 kb on + strand, within BWI76_RS03125 at 645.103 kb on + strand, within BWI76_RS03125 at 645.103 kb on + strand, within BWI76_RS03125 at 645.105 kb on + strand, within BWI76_RS03125 at 645.462 kb on - strand, within BWI76_RS03130 at 645.476 kb on + strand, within BWI76_RS03130 at 645.484 kb on - strand, within BWI76_RS03130 at 645.484 kb on - strand, within BWI76_RS03130 at 645.523 kb on - strand, within BWI76_RS03130 at 645.634 kb on + strand, within BWI76_RS03130 at 645.801 kb on + strand, within BWI76_RS03130 at 645.984 kb on + strand, within BWI76_RS03130 at 646.016 kb on + strand, within BWI76_RS03130 at 646.024 kb on - strand, within BWI76_RS03130
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 643,458 - BWI76_RS03120 0.13 -0.3 643,468 - BWI76_RS03120 0.14 +0.1 643,562 + BWI76_RS03120 0.22 +0.4 643,628 + BWI76_RS03120 0.29 -0.0 643,736 + BWI76_RS03120 0.39 +0.6 643,744 - BWI76_RS03120 0.39 +0.0 643,827 - BWI76_RS03120 0.47 +0.4 644,049 + BWI76_RS03120 0.68 +0.6 644,247 - BWI76_RS03120 0.86 -0.7 644,269 - BWI76_RS03120 0.88 -0.9 644,616 + -1.1 644,699 - BWI76_RS03125 0.36 -0.6 644,908 - BWI76_RS03125 0.62 +1.1 644,965 - BWI76_RS03125 0.69 -0.2 645,084 + BWI76_RS03125 0.83 -0.3 645,103 + BWI76_RS03125 0.85 +0.7 645,103 + BWI76_RS03125 0.85 +1.7 645,105 + BWI76_RS03125 0.86 +3.6 645,462 - BWI76_RS03130 0.26 +0.3 645,476 + BWI76_RS03130 0.27 -0.1 645,484 - BWI76_RS03130 0.28 +0.3 645,484 - BWI76_RS03130 0.28 -0.7 645,523 - BWI76_RS03130 0.33 -0.8 645,634 + BWI76_RS03130 0.45 -0.2 645,801 + BWI76_RS03130 0.64 +0.4 645,984 + BWI76_RS03130 0.85 +0.1 646,016 + BWI76_RS03130 0.88 +0.2 646,024 - BWI76_RS03130 0.89 +0.2
Or see this region's nucleotide sequence