Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4746 and BT4747 are separated by 5 nucleotides BT4747 and BT4748 are separated by 162 nucleotides BT4748 and BT4749 are separated by 81 nucleotides
BT4746: BT4746 - conserved hypothetical protein (NCBI ptt file), at 6,229,336 to 6,229,590
BT4746
BT4747: BT4747 - hypothetical protein (NCBI ptt file), at 6,229,596 to 6,229,925
BT4747
BT4748: BT4748 - hypothetical protein (NCBI ptt file), at 6,230,088 to 6,231,110
BT4748
BT4749: BT4749 - putative DNA methylase (NCBI ptt file), at 6,231,192 to 6,235,154
BT4749
Position (kb)
6230
6231
6232 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 6229.119 kb on + strand at 6229.135 kb on - strand at 6229.300 kb on - strand at 6229.350 kb on - strand at 6229.363 kb on - strand, within BT4746 at 6229.363 kb on - strand, within BT4746 at 6229.395 kb on - strand, within BT4746 at 6229.483 kb on - strand, within BT4746 at 6229.524 kb on - strand, within BT4746 at 6229.526 kb on - strand, within BT4746 at 6229.589 kb on - strand at 6229.589 kb on - strand at 6229.689 kb on - strand, within BT4747 at 6229.689 kb on - strand, within BT4747 at 6229.831 kb on - strand, within BT4747 at 6229.847 kb on + strand, within BT4747 at 6229.928 kb on + strand at 6229.928 kb on + strand at 6230.089 kb on + strand at 6230.259 kb on - strand, within BT4748 at 6230.341 kb on - strand, within BT4748 at 6230.414 kb on + strand at 6230.414 kb on + strand, within BT4748 at 6230.415 kb on - strand, within BT4748 at 6230.486 kb on + strand, within BT4748 at 6230.488 kb on + strand, within BT4748 at 6230.489 kb on - strand, within BT4748 at 6230.489 kb on - strand, within BT4748 at 6230.607 kb on - strand, within BT4748 at 6230.844 kb on - strand, within BT4748 at 6230.944 kb on + strand, within BT4748 at 6230.945 kb on - strand, within BT4748 at 6230.945 kb on - strand, within BT4748 at 6230.976 kb on + strand, within BT4748 at 6231.124 kb on + strand at 6231.125 kb on - strand at 6231.140 kb on + strand at 6231.199 kb on - strand at 6231.374 kb on + strand at 6231.433 kb on + strand at 6231.434 kb on - strand at 6231.438 kb on - strand at 6231.482 kb on - strand at 6231.482 kb on - strand at 6231.485 kb on + strand at 6231.485 kb on + strand at 6231.486 kb on - strand at 6231.490 kb on + strand at 6231.490 kb on + strand at 6231.558 kb on + strand at 6231.592 kb on + strand, within BT4749 at 6231.655 kb on + strand, within BT4749 at 6231.726 kb on + strand, within BT4749 at 6231.726 kb on + strand, within BT4749 at 6231.727 kb on - strand, within BT4749 at 6231.734 kb on - strand, within BT4749 at 6231.734 kb on - strand, within BT4749
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 6,229,119 + +0.1 6,229,135 - -0.5 6,229,300 - +1.4 6,229,350 - +0.5 6,229,363 - BT4746 0.11 -2.4 6,229,363 - BT4746 0.11 +0.9 6,229,395 - BT4746 0.23 +1.4 6,229,483 - BT4746 0.58 -0.5 6,229,524 - BT4746 0.74 -1.1 6,229,526 - BT4746 0.75 +1.9 6,229,589 - -1.9 6,229,589 - -2.1 6,229,689 - BT4747 0.28 -3.1 6,229,689 - BT4747 0.28 -0.1 6,229,831 - BT4747 0.71 -0.8 6,229,847 + BT4747 0.76 -0.1 6,229,928 + -1.1 6,229,928 + -1.1 6,230,089 + +0.9 6,230,259 - BT4748 0.17 +1.1 6,230,341 - BT4748 0.25 +0.9 6,230,414 + -1.1 6,230,414 + BT4748 0.32 -2.1 6,230,415 - BT4748 0.32 +0.8 6,230,486 + BT4748 0.39 +1.6 6,230,488 + BT4748 0.39 -2.1 6,230,489 - BT4748 0.39 -1.6 6,230,489 - BT4748 0.39 -5.2 6,230,607 - BT4748 0.51 -1.1 6,230,844 - BT4748 0.74 +0.5 6,230,944 + BT4748 0.84 -0.1 6,230,945 - BT4748 0.84 -1.1 6,230,945 - BT4748 0.84 -0.5 6,230,976 + BT4748 0.87 +1.3 6,231,124 + -1.1 6,231,125 - -3.2 6,231,140 + -0.6 6,231,199 - -1.6 6,231,374 + -2.6 6,231,433 + -0.5 6,231,434 - -1.6 6,231,438 - -1.1 6,231,482 - -0.8 6,231,482 - -1.7 6,231,485 + +1.3 6,231,485 + -1.8 6,231,486 - +0.7 6,231,490 + -1.2 6,231,490 + +1.5 6,231,558 + -0.6 6,231,592 + BT4749 0.10 +0.5 6,231,655 + BT4749 0.12 +2.3 6,231,726 + BT4749 0.13 -0.4 6,231,726 + BT4749 0.13 -0.3 6,231,727 - BT4749 0.13 -0.1 6,231,734 - BT4749 0.14 -1.1 6,231,734 - BT4749 0.14 +0.4
Or see this region's nucleotide sequence