Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4304 and BT4305 are separated by 13 nucleotides BT4305 and BT4306 are separated by 144 nucleotides
BT4304: BT4304 - putative glycosyltransferase (NCBI ptt file), at 5,672,344 to 5,673,618
BT4304
BT4305: BT4305 - putative alpha-amylase (NCBI ptt file), at 5,673,632 to 5,675,014
BT4305
BT4306: BT4306 - hypothetical protein (NCBI ptt file), at 5,675,159 to 5,676,787
BT4306
Position (kb)
5673
5674
5675
5676 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 5672.647 kb on - strand, within BT4304 at 5672.712 kb on + strand, within BT4304 at 5672.802 kb on - strand, within BT4304 at 5672.802 kb on - strand, within BT4304 at 5672.893 kb on + strand, within BT4304 at 5672.893 kb on + strand, within BT4304 at 5672.894 kb on - strand, within BT4304 at 5672.973 kb on + strand, within BT4304 at 5672.973 kb on + strand, within BT4304 at 5672.973 kb on + strand, within BT4304 at 5672.974 kb on - strand, within BT4304 at 5673.003 kb on + strand, within BT4304 at 5673.100 kb on - strand, within BT4304 at 5673.100 kb on - strand, within BT4304 at 5673.103 kb on - strand, within BT4304 at 5673.125 kb on - strand, within BT4304 at 5673.132 kb on - strand, within BT4304 at 5673.199 kb on - strand, within BT4304 at 5673.208 kb on - strand, within BT4304 at 5673.311 kb on + strand, within BT4304 at 5673.312 kb on - strand, within BT4304 at 5673.319 kb on - strand, within BT4304 at 5673.346 kb on - strand, within BT4304 at 5673.364 kb on - strand, within BT4304 at 5673.373 kb on - strand, within BT4304 at 5673.377 kb on + strand, within BT4304 at 5673.387 kb on + strand, within BT4304 at 5673.389 kb on + strand, within BT4304 at 5673.389 kb on + strand, within BT4304 at 5673.389 kb on + strand, within BT4304 at 5673.442 kb on + strand, within BT4304 at 5673.459 kb on + strand, within BT4304 at 5673.459 kb on + strand, within BT4304 at 5673.480 kb on + strand, within BT4304 at 5673.481 kb on - strand, within BT4304 at 5673.487 kb on - strand, within BT4304 at 5673.487 kb on - strand, within BT4304 at 5673.487 kb on - strand, within BT4304 at 5673.499 kb on - strand at 5673.502 kb on + strand at 5673.502 kb on + strand at 5673.503 kb on - strand at 5673.526 kb on - strand at 5673.546 kb on + strand at 5673.626 kb on + strand at 5673.627 kb on - strand at 5673.627 kb on - strand at 5673.627 kb on - strand at 5673.671 kb on - strand at 5673.671 kb on - strand at 5673.716 kb on + strand at 5673.717 kb on - strand at 5673.719 kb on + strand at 5673.719 kb on + strand at 5673.773 kb on + strand, within BT4305 at 5673.774 kb on - strand, within BT4305 at 5673.843 kb on - strand, within BT4305 at 5674.077 kb on - strand, within BT4305 at 5674.149 kb on + strand, within BT4305 at 5674.150 kb on - strand, within BT4305 at 5674.173 kb on - strand, within BT4305 at 5674.173 kb on - strand, within BT4305 at 5674.173 kb on - strand, within BT4305 at 5674.173 kb on - strand, within BT4305 at 5674.176 kb on + strand, within BT4305 at 5674.176 kb on + strand, within BT4305 at 5674.176 kb on + strand, within BT4305 at 5674.176 kb on + strand, within BT4305 at 5674.176 kb on + strand, within BT4305 at 5674.177 kb on - strand, within BT4305 at 5674.177 kb on - strand, within BT4305 at 5674.211 kb on + strand, within BT4305 at 5674.212 kb on + strand, within BT4305 at 5674.277 kb on + strand, within BT4305 at 5674.298 kb on + strand, within BT4305 at 5674.299 kb on - strand, within BT4305 at 5674.299 kb on - strand, within BT4305 at 5674.299 kb on - strand, within BT4305 at 5674.301 kb on - strand, within BT4305 at 5674.343 kb on - strand, within BT4305 at 5674.345 kb on + strand, within BT4305 at 5674.345 kb on + strand, within BT4305 at 5674.359 kb on + strand, within BT4305 at 5674.445 kb on - strand, within BT4305 at 5674.478 kb on - strand, within BT4305 at 5674.544 kb on - strand, within BT4305 at 5674.595 kb on + strand, within BT4305 at 5674.595 kb on + strand, within BT4305 at 5674.595 kb on + strand, within BT4305 at 5674.596 kb on - strand, within BT4305 at 5674.596 kb on - strand, within BT4305 at 5674.637 kb on - strand, within BT4305 at 5674.655 kb on + strand, within BT4305 at 5674.705 kb on + strand, within BT4305 at 5674.727 kb on + strand, within BT4305 at 5674.728 kb on - strand, within BT4305 at 5674.728 kb on - strand, within BT4305 at 5674.757 kb on + strand, within BT4305 at 5674.758 kb on - strand, within BT4305 at 5674.766 kb on - strand, within BT4305 at 5674.873 kb on - strand, within BT4305 at 5674.930 kb on - strand at 5675.018 kb on - strand at 5675.024 kb on - strand at 5675.078 kb on - strand at 5675.081 kb on + strand at 5675.092 kb on - strand at 5675.101 kb on + strand at 5675.144 kb on - strand at 5675.148 kb on + strand at 5675.151 kb on - strand at 5675.153 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 5,672,647 - BT4304 0.24 -1.9 5,672,712 + BT4304 0.29 -3.8 5,672,802 - BT4304 0.36 -1.2 5,672,802 - BT4304 0.36 -3.9 5,672,893 + BT4304 0.43 -3.8 5,672,893 + BT4304 0.43 -3.6 5,672,894 - BT4304 0.43 -4.4 5,672,973 + BT4304 0.49 -3.9 5,672,973 + BT4304 0.49 -1.5 5,672,973 + BT4304 0.49 -4.5 5,672,974 - BT4304 0.49 -3.7 5,673,003 + BT4304 0.52 +0.1 5,673,100 - BT4304 0.59 -3.9 5,673,100 - BT4304 0.59 -0.5 5,673,103 - BT4304 0.60 -4.0 5,673,125 - BT4304 0.61 -0.9 5,673,132 - BT4304 0.62 -2.7 5,673,199 - BT4304 0.67 -3.7 5,673,208 - BT4304 0.68 -3.5 5,673,311 + BT4304 0.76 -2.9 5,673,312 - BT4304 0.76 -2.7 5,673,319 - BT4304 0.76 -4.0 5,673,346 - BT4304 0.79 -1.5 5,673,364 - BT4304 0.80 -4.5 5,673,373 - BT4304 0.81 -2.7 5,673,377 + BT4304 0.81 -1.4 5,673,387 + BT4304 0.82 -0.9 5,673,389 + BT4304 0.82 -1.9 5,673,389 + BT4304 0.82 -1.5 5,673,389 + BT4304 0.82 -2.3 5,673,442 + BT4304 0.86 -5.6 5,673,459 + BT4304 0.87 -3.0 5,673,459 + BT4304 0.87 -3.8 5,673,480 + BT4304 0.89 -2.9 5,673,481 - BT4304 0.89 +0.1 5,673,487 - BT4304 0.90 -2.8 5,673,487 - BT4304 0.90 -5.0 5,673,487 - BT4304 0.90 -3.0 5,673,499 - +0.1 5,673,502 + -4.1 5,673,502 + -0.0 5,673,503 - +0.1 5,673,526 - -3.1 5,673,546 + -0.5 5,673,626 + +1.3 5,673,627 - +1.2 5,673,627 - -4.0 5,673,627 - -3.5 5,673,671 - -3.3 5,673,671 - -3.1 5,673,716 + -4.6 5,673,717 - -1.5 5,673,719 + +1.1 5,673,719 + -0.5 5,673,773 + BT4305 0.10 -1.8 5,673,774 - BT4305 0.10 +1.1 5,673,843 - BT4305 0.15 -3.1 5,674,077 - BT4305 0.32 -3.1 5,674,149 + BT4305 0.37 -3.5 5,674,150 - BT4305 0.37 +1.1 5,674,173 - BT4305 0.39 +0.6 5,674,173 - BT4305 0.39 -4.1 5,674,173 - BT4305 0.39 +0.1 5,674,173 - BT4305 0.39 -5.9 5,674,176 + BT4305 0.39 -2.2 5,674,176 + BT4305 0.39 -0.9 5,674,176 + BT4305 0.39 -0.9 5,674,176 + BT4305 0.39 +0.8 5,674,176 + BT4305 0.39 +1.1 5,674,177 - BT4305 0.39 -2.0 5,674,177 - BT4305 0.39 -2.0 5,674,211 + BT4305 0.42 -4.3 5,674,212 + BT4305 0.42 -1.9 5,674,277 + BT4305 0.47 -2.8 5,674,298 + BT4305 0.48 -0.9 5,674,299 - BT4305 0.48 +0.1 5,674,299 - BT4305 0.48 -3.5 5,674,299 - BT4305 0.48 -2.2 5,674,301 - BT4305 0.48 -2.1 5,674,343 - BT4305 0.51 -0.9 5,674,345 + BT4305 0.52 -2.3 5,674,345 + BT4305 0.52 -2.2 5,674,359 + BT4305 0.53 -2.2 5,674,445 - BT4305 0.59 +0.8 5,674,478 - BT4305 0.61 -0.9 5,674,544 - BT4305 0.66 -0.8 5,674,595 + BT4305 0.70 -2.3 5,674,595 + BT4305 0.70 -4.0 5,674,595 + BT4305 0.70 +0.1 5,674,596 - BT4305 0.70 +2.7 5,674,596 - BT4305 0.70 -2.1 5,674,637 - BT4305 0.73 -2.1 5,674,655 + BT4305 0.74 -2.9 5,674,705 + BT4305 0.78 +3.3 5,674,727 + BT4305 0.79 -3.0 5,674,728 - BT4305 0.79 -3.8 5,674,728 - BT4305 0.79 -3.2 5,674,757 + BT4305 0.81 -1.7 5,674,758 - BT4305 0.81 +2.3 5,674,766 - BT4305 0.82 +1.4 5,674,873 - BT4305 0.90 -1.3 5,674,930 - -0.9 5,675,018 - -0.4 5,675,024 - -0.8 5,675,078 - -0.8 5,675,081 + -2.2 5,675,092 - -1.0 5,675,101 + +0.4 5,675,144 - -0.9 5,675,148 + +1.1 5,675,151 - -0.6 5,675,153 - +3.3
Or see this region's nucleotide sequence