Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3904 and BT3905 are separated by 78 nucleotides BT3905 and BT3906 are separated by 236 nucleotides
BT3904: BT3904 - conserved hypothetical protein (NCBI ptt file), at 5,075,452 to 5,076,444
BT3904
BT3905: BT3905 - putative alkaline protease aprF (NCBI ptt file), at 5,076,523 to 5,077,986
BT3905
BT3906: BT3906 - conserved hypothetical protein (NCBI ptt file), at 5,078,223 to 5,079,551
BT3906
Position (kb)
5076
5077
5078 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 5075.551 kb on - strand at 5075.576 kb on + strand, within BT3904 at 5075.577 kb on - strand, within BT3904 at 5075.584 kb on + strand, within BT3904 at 5075.622 kb on + strand, within BT3904 at 5075.832 kb on - strand, within BT3904 at 5075.925 kb on + strand, within BT3904 at 5075.925 kb on + strand, within BT3904 at 5075.925 kb on + strand, within BT3904 at 5075.926 kb on - strand, within BT3904 at 5075.926 kb on - strand, within BT3904 at 5075.926 kb on - strand, within BT3904 at 5075.927 kb on + strand, within BT3904 at 5076.041 kb on + strand, within BT3904 at 5076.382 kb on - strand at 5076.444 kb on + strand at 5076.447 kb on + strand at 5076.500 kb on - strand at 5076.524 kb on + strand at 5076.706 kb on + strand, within BT3905 at 5076.724 kb on + strand, within BT3905 at 5076.810 kb on - strand, within BT3905 at 5076.876 kb on + strand, within BT3905 at 5076.880 kb on + strand, within BT3905 at 5076.880 kb on + strand, within BT3905 at 5076.880 kb on + strand, within BT3905 at 5076.881 kb on - strand, within BT3905 at 5076.886 kb on + strand, within BT3905 at 5076.887 kb on - strand, within BT3905 at 5076.960 kb on + strand, within BT3905 at 5077.055 kb on - strand, within BT3905 at 5077.174 kb on + strand, within BT3905 at 5077.175 kb on - strand, within BT3905 at 5077.343 kb on + strand, within BT3905 at 5077.344 kb on - strand, within BT3905 at 5077.391 kb on + strand, within BT3905 at 5077.392 kb on - strand, within BT3905 at 5077.479 kb on + strand, within BT3905 at 5077.505 kb on + strand, within BT3905 at 5077.505 kb on + strand, within BT3905 at 5077.505 kb on + strand, within BT3905 at 5077.540 kb on + strand, within BT3905 at 5077.778 kb on + strand, within BT3905 at 5077.895 kb on - strand at 5077.895 kb on - strand at 5077.912 kb on + strand at 5077.995 kb on - strand at 5078.001 kb on + strand at 5078.013 kb on - strand at 5078.023 kb on + strand at 5078.189 kb on + strand at 5078.190 kb on - strand at 5078.312 kb on + strand at 5078.333 kb on + strand at 5078.408 kb on + strand, within BT3906 at 5078.408 kb on + strand, within BT3906 at 5078.409 kb on - strand, within BT3906 at 5078.451 kb on - strand, within BT3906 at 5078.475 kb on - strand, within BT3906 at 5078.478 kb on + strand, within BT3906 at 5078.729 kb on + strand, within BT3906 at 5078.729 kb on + strand, within BT3906 at 5078.730 kb on - strand, within BT3906 at 5078.730 kb on - strand, within BT3906 at 5078.730 kb on - strand, within BT3906 at 5078.795 kb on - strand, within BT3906 at 5078.838 kb on + strand, within BT3906 at 5078.869 kb on - strand, within BT3906
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 5,075,551 - -1.5 5,075,576 + BT3904 0.12 -3.6 5,075,577 - BT3904 0.13 -1.9 5,075,584 + BT3904 0.13 -1.5 5,075,622 + BT3904 0.17 -2.2 5,075,832 - BT3904 0.38 -2.4 5,075,925 + BT3904 0.48 -1.5 5,075,925 + BT3904 0.48 -2.5 5,075,925 + BT3904 0.48 -1.5 5,075,926 - BT3904 0.48 +0.1 5,075,926 - BT3904 0.48 -4.1 5,075,926 - BT3904 0.48 -2.7 5,075,927 + BT3904 0.48 -3.2 5,076,041 + BT3904 0.59 -2.9 5,076,382 - -4.1 5,076,444 + -3.5 5,076,447 + -3.1 5,076,500 - -0.3 5,076,524 + -0.9 5,076,706 + BT3905 0.12 -2.2 5,076,724 + BT3905 0.14 -0.9 5,076,810 - BT3905 0.20 -0.9 5,076,876 + BT3905 0.24 -0.9 5,076,880 + BT3905 0.24 -1.9 5,076,880 + BT3905 0.24 -1.5 5,076,880 + BT3905 0.24 -0.9 5,076,881 - BT3905 0.24 +0.1 5,076,886 + BT3905 0.25 -2.9 5,076,887 - BT3905 0.25 +0.1 5,076,960 + BT3905 0.30 -1.9 5,077,055 - BT3905 0.36 -1.9 5,077,174 + BT3905 0.44 -2.5 5,077,175 - BT3905 0.45 -3.8 5,077,343 + BT3905 0.56 +0.1 5,077,344 - BT3905 0.56 -2.2 5,077,391 + BT3905 0.59 -3.8 5,077,392 - BT3905 0.59 +0.1 5,077,479 + BT3905 0.65 -0.5 5,077,505 + BT3905 0.67 -3.8 5,077,505 + BT3905 0.67 +0.1 5,077,505 + BT3905 0.67 -3.1 5,077,540 + BT3905 0.69 -1.5 5,077,778 + BT3905 0.86 -2.5 5,077,895 - -3.1 5,077,895 - -2.2 5,077,912 + -0.2 5,077,995 - +0.4 5,078,001 + +0.1 5,078,013 - +0.4 5,078,023 + -5.9 5,078,189 + -2.5 5,078,190 - +1.8 5,078,312 + +0.1 5,078,333 + -1.2 5,078,408 + BT3906 0.14 -0.9 5,078,408 + BT3906 0.14 +0.7 5,078,409 - BT3906 0.14 +0.1 5,078,451 - BT3906 0.17 -0.9 5,078,475 - BT3906 0.19 +1.4 5,078,478 + BT3906 0.19 -1.9 5,078,729 + BT3906 0.38 +0.5 5,078,729 + BT3906 0.38 -0.2 5,078,730 - BT3906 0.38 +2.1 5,078,730 - BT3906 0.38 +0.1 5,078,730 - BT3906 0.38 +2.1 5,078,795 - BT3906 0.43 -0.2 5,078,838 + BT3906 0.46 -2.5 5,078,869 - BT3906 0.49 -1.2
Or see this region's nucleotide sequence