Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2780 and tRNA-Leu are separated by 183 nucleotides tRNA-Leu and BT2781 are separated by 164 nucleotides BT2781 and BT2782 are separated by 88 nucleotides
BT2780: BT2780 - conserved hypothetical protein, putative membrane-associated protein (NCBI ptt file), at 3,447,271 to 3,448,458
BT2780
BT_t49: tRNA-Leu - tRNA-Leu (RefSeq), at 3,448,642 to 3,448,728
tRNA-Leu
BT2781: BT2781 - putative mannose-1-phosphate guanylyltransferase (NCBI ptt file), at 3,448,893 to 3,449,975
BT2781
BT2782: BT2782 - long-chain-fatty-acid--CoA ligase (NCBI ptt file), at 3,450,064 to 3,451,713
BT2782
Position (kb)
3448
3449
3450 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3447.902 kb on + strand, within BT2780 at 3447.919 kb on - strand, within BT2780 at 3447.944 kb on + strand, within BT2780 at 3447.944 kb on + strand, within BT2780 at 3447.945 kb on - strand, within BT2780 at 3447.948 kb on + strand, within BT2780 at 3447.948 kb on + strand, within BT2780 at 3447.949 kb on - strand, within BT2780 at 3447.954 kb on - strand, within BT2780 at 3448.008 kb on + strand, within BT2780 at 3448.058 kb on + strand, within BT2780 at 3448.062 kb on + strand, within BT2780 at 3448.062 kb on + strand, within BT2780 at 3448.065 kb on - strand, within BT2780 at 3448.077 kb on + strand, within BT2780 at 3448.078 kb on - strand, within BT2780 at 3448.100 kb on + strand, within BT2780 at 3448.115 kb on + strand, within BT2780 at 3448.134 kb on + strand, within BT2780 at 3448.134 kb on + strand, within BT2780 at 3448.144 kb on - strand, within BT2780 at 3448.165 kb on - strand, within BT2780 at 3448.165 kb on - strand, within BT2780 at 3448.170 kb on - strand, within BT2780 at 3448.170 kb on - strand, within BT2780 at 3448.181 kb on - strand, within BT2780 at 3448.246 kb on + strand, within BT2780 at 3448.246 kb on + strand, within BT2780 at 3448.311 kb on + strand, within BT2780 at 3448.311 kb on + strand, within BT2780 at 3448.312 kb on - strand, within BT2780 at 3448.312 kb on - strand, within BT2780 at 3448.335 kb on + strand, within BT2780 at 3448.343 kb on + strand at 3448.359 kb on - strand at 3448.359 kb on - strand at 3448.409 kb on + strand at 3448.409 kb on + strand at 3448.410 kb on - strand at 3448.445 kb on + strand at 3448.445 kb on + strand at 3448.446 kb on - strand at 3448.456 kb on + strand at 3448.456 kb on + strand at 3448.457 kb on - strand at 3448.534 kb on + strand at 3448.535 kb on - strand at 3448.535 kb on - strand at 3448.535 kb on - strand at 3448.581 kb on - strand at 3448.591 kb on - strand at 3448.595 kb on + strand at 3448.596 kb on - strand at 3448.784 kb on - strand at 3448.784 kb on - strand at 3448.842 kb on + strand at 3448.889 kb on + strand at 3448.908 kb on - strand at 3449.367 kb on + strand, within BT2781 at 3449.580 kb on + strand, within BT2781 at 3449.580 kb on + strand, within BT2781 at 3449.677 kb on + strand, within BT2781 at 3449.684 kb on - strand, within BT2781 at 3449.733 kb on + strand, within BT2781 at 3449.931 kb on + strand at 3450.013 kb on - strand at 3450.013 kb on - strand at 3450.022 kb on - strand at 3450.061 kb on + strand at 3450.070 kb on - strand at 3450.176 kb on + strand at 3450.189 kb on + strand at 3450.190 kb on - strand at 3450.191 kb on + strand at 3450.204 kb on + strand at 3450.210 kb on + strand at 3450.211 kb on - strand at 3450.265 kb on - strand, within BT2782 at 3450.265 kb on - strand, within BT2782 at 3450.288 kb on - strand, within BT2782 at 3450.288 kb on - strand, within BT2782 at 3450.311 kb on - strand, within BT2782 at 3450.340 kb on - strand, within BT2782 at 3450.347 kb on - strand, within BT2782 at 3450.369 kb on - strand, within BT2782 at 3450.369 kb on - strand, within BT2782 at 3450.372 kb on + strand at 3450.390 kb on + strand, within BT2782 at 3450.396 kb on + strand, within BT2782 at 3450.449 kb on - strand, within BT2782 at 3450.472 kb on - strand, within BT2782 at 3450.516 kb on - strand, within BT2782 at 3450.528 kb on + strand, within BT2782 at 3450.529 kb on - strand, within BT2782 at 3450.531 kb on + strand, within BT2782 at 3450.531 kb on + strand, within BT2782 at 3450.532 kb on - strand, within BT2782 at 3450.559 kb on + strand, within BT2782 at 3450.560 kb on - strand, within BT2782 at 3450.632 kb on - strand, within BT2782 at 3450.636 kb on + strand, within BT2782 at 3450.639 kb on + strand, within BT2782 at 3450.646 kb on + strand, within BT2782 at 3450.647 kb on - strand, within BT2782 at 3450.682 kb on - strand, within BT2782 at 3450.709 kb on + strand, within BT2782 at 3450.728 kb on + strand, within BT2782 at 3450.728 kb on + strand, within BT2782 at 3450.729 kb on - strand, within BT2782 at 3450.729 kb on - strand, within BT2782 at 3450.729 kb on - strand, within BT2782 at 3450.742 kb on + strand, within BT2782 at 3450.785 kb on - strand, within BT2782 at 3450.807 kb on + strand, within BT2782 at 3450.807 kb on + strand, within BT2782 at 3450.876 kb on + strand, within BT2782 at 3450.929 kb on - strand, within BT2782 at 3450.942 kb on + strand, within BT2782 at 3450.942 kb on + strand, within BT2782
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 3,447,902 + BT2780 0.53 -1.6 3,447,919 - BT2780 0.55 +1.0 3,447,944 + BT2780 0.57 +2.0 3,447,944 + BT2780 0.57 -1.0 3,447,945 - BT2780 0.57 -1.1 3,447,948 + BT2780 0.57 -0.6 3,447,948 + BT2780 0.57 -0.3 3,447,949 - BT2780 0.57 -4.1 3,447,954 - BT2780 0.57 +2.3 3,448,008 + BT2780 0.62 -1.8 3,448,058 + BT2780 0.66 +1.0 3,448,062 + BT2780 0.67 -1.0 3,448,062 + BT2780 0.67 -0.6 3,448,065 - BT2780 0.67 -1.6 3,448,077 + BT2780 0.68 -0.4 3,448,078 - BT2780 0.68 +1.5 3,448,100 + BT2780 0.70 -0.1 3,448,115 + BT2780 0.71 -0.0 3,448,134 + BT2780 0.73 -1.7 3,448,134 + BT2780 0.73 -1.9 3,448,144 - BT2780 0.73 -1.0 3,448,165 - BT2780 0.75 -3.9 3,448,165 - BT2780 0.75 -4.2 3,448,170 - BT2780 0.76 -0.6 3,448,170 - BT2780 0.76 +1.7 3,448,181 - BT2780 0.77 +0.7 3,448,246 + BT2780 0.82 -2.0 3,448,246 + BT2780 0.82 -0.4 3,448,311 + BT2780 0.88 +0.2 3,448,311 + BT2780 0.88 -1.9 3,448,312 - BT2780 0.88 -2.4 3,448,312 - BT2780 0.88 -1.0 3,448,335 + BT2780 0.90 -1.8 3,448,343 + -1.0 3,448,359 - -3.0 3,448,359 - +1.0 3,448,409 + -0.8 3,448,409 + -0.8 3,448,410 - +2.1 3,448,445 + -1.0 3,448,445 + -1.6 3,448,446 - +1.2 3,448,456 + -2.7 3,448,456 + -0.8 3,448,457 - -0.6 3,448,534 + -1.2 3,448,535 - -1.6 3,448,535 - -1.9 3,448,535 - -2.6 3,448,581 - -0.0 3,448,591 - -3.0 3,448,595 + -1.0 3,448,596 - -0.0 3,448,784 - -0.0 3,448,784 - -2.0 3,448,842 + +0.4 3,448,889 + -4.2 3,448,908 - -3.8 3,449,367 + BT2781 0.44 -3.2 3,449,580 + BT2781 0.63 -3.9 3,449,580 + BT2781 0.63 -3.2 3,449,677 + BT2781 0.72 -1.7 3,449,684 - BT2781 0.73 -2.8 3,449,733 + BT2781 0.78 -1.8 3,449,931 + -1.6 3,450,013 - -1.4 3,450,013 - -1.3 3,450,022 - -2.8 3,450,061 + +0.1 3,450,070 - +0.4 3,450,176 + -0.5 3,450,189 + -2.2 3,450,190 - -3.0 3,450,191 + +1.4 3,450,204 + -2.6 3,450,210 + +2.7 3,450,211 - -0.0 3,450,265 - BT2782 0.12 +0.5 3,450,265 - BT2782 0.12 +0.5 3,450,288 - BT2782 0.14 -1.3 3,450,288 - BT2782 0.14 -0.5 3,450,311 - BT2782 0.15 -2.6 3,450,340 - BT2782 0.17 -0.7 3,450,347 - BT2782 0.17 -2.6 3,450,369 - BT2782 0.18 +1.3 3,450,369 - BT2782 0.18 -1.2 3,450,372 + -0.0 3,450,390 + BT2782 0.20 +2.8 3,450,396 + BT2782 0.20 -1.8 3,450,449 - BT2782 0.23 +1.0 3,450,472 - BT2782 0.25 -2.6 3,450,516 - BT2782 0.27 -0.5 3,450,528 + BT2782 0.28 -0.8 3,450,529 - BT2782 0.28 -1.6 3,450,531 + BT2782 0.28 +1.9 3,450,531 + BT2782 0.28 +2.5 3,450,532 - BT2782 0.28 +1.8 3,450,559 + BT2782 0.30 +1.3 3,450,560 - BT2782 0.30 -1.6 3,450,632 - BT2782 0.34 +3.5 3,450,636 + BT2782 0.35 -0.0 3,450,639 + BT2782 0.35 +2.0 3,450,646 + BT2782 0.35 -1.9 3,450,647 - BT2782 0.35 -1.6 3,450,682 - BT2782 0.37 -1.7 3,450,709 + BT2782 0.39 +0.5 3,450,728 + BT2782 0.40 +1.7 3,450,728 + BT2782 0.40 -2.4 3,450,729 - BT2782 0.40 -0.0 3,450,729 - BT2782 0.40 -0.6 3,450,729 - BT2782 0.40 -0.5 3,450,742 + BT2782 0.41 -0.1 3,450,785 - BT2782 0.44 -2.5 3,450,807 + BT2782 0.45 -1.6 3,450,807 + BT2782 0.45 +1.0 3,450,876 + BT2782 0.49 -1.4 3,450,929 - BT2782 0.52 -1.1 3,450,942 + BT2782 0.53 -3.2 3,450,942 + BT2782 0.53 +3.0
Or see this region's nucleotide sequence