Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2675 and BT2676 are separated by 96 nucleotides BT2676 and BT2677 are separated by 266 nucleotides
BT2675: BT2675 - hypothetical protein (NCBI ptt file), at 3,334,148 to 3,334,873
BT2675
BT2676: BT2676 - hypothetical protein (NCBI ptt file), at 3,334,970 to 3,335,602
BT2676
BT2677: BT2677 - hypothetical protein (NCBI ptt file), at 3,335,869 to 3,336,192
BT2677
Position (kb)
3334
3335 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3333.405 kb on - strand at 3333.408 kb on - strand at 3333.522 kb on + strand at 3333.556 kb on + strand at 3333.600 kb on - strand at 3333.608 kb on - strand at 3333.695 kb on - strand at 3333.718 kb on + strand at 3333.760 kb on + strand at 3333.823 kb on + strand at 3333.839 kb on - strand at 3333.843 kb on - strand at 3333.851 kb on + strand at 3333.874 kb on - strand at 3333.906 kb on + strand at 3334.081 kb on + strand at 3334.100 kb on - strand at 3334.111 kb on - strand at 3334.206 kb on + strand at 3334.310 kb on + strand, within BT2675 at 3334.310 kb on + strand, within BT2675 at 3334.388 kb on + strand, within BT2675 at 3334.388 kb on + strand, within BT2675 at 3334.442 kb on + strand, within BT2675 at 3334.442 kb on + strand, within BT2675 at 3334.472 kb on + strand, within BT2675 at 3334.472 kb on + strand, within BT2675 at 3334.474 kb on + strand, within BT2675 at 3334.549 kb on + strand, within BT2675 at 3334.550 kb on - strand, within BT2675 at 3334.554 kb on + strand, within BT2675 at 3334.555 kb on - strand, within BT2675 at 3334.557 kb on + strand, within BT2675 at 3334.557 kb on + strand, within BT2675 at 3334.591 kb on + strand, within BT2675 at 3334.592 kb on - strand, within BT2675 at 3334.609 kb on + strand, within BT2675 at 3334.610 kb on - strand, within BT2675 at 3334.631 kb on + strand, within BT2675 at 3334.631 kb on + strand at 3334.632 kb on - strand, within BT2675 at 3334.632 kb on - strand, within BT2675 at 3334.693 kb on + strand, within BT2675 at 3334.745 kb on + strand, within BT2675 at 3334.746 kb on - strand, within BT2675 at 3334.749 kb on + strand, within BT2675 at 3334.750 kb on - strand, within BT2675 at 3334.750 kb on - strand, within BT2675 at 3334.777 kb on - strand, within BT2675 at 3334.947 kb on + strand at 3334.953 kb on + strand at 3334.953 kb on + strand at 3334.958 kb on + strand at 3334.958 kb on + strand at 3335.006 kb on - strand at 3335.033 kb on + strand at 3335.082 kb on + strand, within BT2676 at 3335.082 kb on + strand, within BT2676 at 3335.101 kb on + strand, within BT2676 at 3335.102 kb on - strand, within BT2676 at 3335.102 kb on - strand, within BT2676 at 3335.127 kb on + strand, within BT2676 at 3335.143 kb on - strand, within BT2676 at 3335.206 kb on - strand, within BT2676 at 3335.240 kb on - strand, within BT2676 at 3335.294 kb on - strand, within BT2676 at 3335.294 kb on - strand, within BT2676 at 3335.306 kb on - strand, within BT2676 at 3335.326 kb on + strand, within BT2676 at 3335.365 kb on - strand, within BT2676 at 3335.412 kb on + strand, within BT2676 at 3335.419 kb on + strand, within BT2676 at 3335.538 kb on - strand, within BT2676 at 3335.588 kb on + strand at 3335.603 kb on + strand at 3335.611 kb on - strand at 3335.663 kb on + strand at 3335.664 kb on - strand at 3335.664 kb on - strand at 3335.694 kb on + strand at 3335.768 kb on + strand at 3335.769 kb on - strand at 3335.821 kb on - strand at 3335.821 kb on - strand at 3335.851 kb on + strand at 3335.851 kb on + strand at 3335.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 3,333,405 - -4.1 3,333,408 - -1.2 3,333,522 + -0.8 3,333,556 + +1.9 3,333,600 - -1.6 3,333,608 - -0.0 3,333,695 - -1.2 3,333,718 + -1.2 3,333,760 + +1.8 3,333,823 + -1.6 3,333,839 - -0.7 3,333,843 - +0.6 3,333,851 + -0.9 3,333,874 - -1.6 3,333,906 + +1.0 3,334,081 + +0.5 3,334,100 - -0.7 3,334,111 - -0.0 3,334,206 + +0.7 3,334,310 + BT2675 0.22 +2.8 3,334,310 + BT2675 0.22 +0.7 3,334,388 + BT2675 0.33 +0.1 3,334,388 + BT2675 0.33 +2.0 3,334,442 + BT2675 0.40 -0.9 3,334,442 + BT2675 0.40 -0.6 3,334,472 + BT2675 0.45 +2.6 3,334,472 + BT2675 0.45 -1.4 3,334,474 + BT2675 0.45 -0.3 3,334,549 + BT2675 0.55 -1.8 3,334,550 - BT2675 0.55 +1.9 3,334,554 + BT2675 0.56 +2.7 3,334,555 - BT2675 0.56 -0.5 3,334,557 + BT2675 0.56 +1.5 3,334,557 + BT2675 0.56 -0.7 3,334,591 + BT2675 0.61 +2.0 3,334,592 - BT2675 0.61 -0.9 3,334,609 + BT2675 0.63 -0.6 3,334,610 - BT2675 0.64 +0.7 3,334,631 + BT2675 0.67 -2.8 3,334,631 + -3.0 3,334,632 - BT2675 0.67 -1.2 3,334,632 - BT2675 0.67 -2.0 3,334,693 + BT2675 0.75 -2.2 3,334,745 + BT2675 0.82 +2.0 3,334,746 - BT2675 0.82 -0.7 3,334,749 + BT2675 0.83 +1.8 3,334,750 - BT2675 0.83 -1.4 3,334,750 - BT2675 0.83 +1.3 3,334,777 - BT2675 0.87 -1.2 3,334,947 + -1.0 3,334,953 + -0.8 3,334,953 + -1.0 3,334,958 + +0.6 3,334,958 + -0.8 3,335,006 - -1.4 3,335,033 + +0.4 3,335,082 + BT2676 0.18 -1.0 3,335,082 + BT2676 0.18 -1.0 3,335,101 + BT2676 0.21 -2.1 3,335,102 - BT2676 0.21 -0.0 3,335,102 - BT2676 0.21 -0.6 3,335,127 + BT2676 0.25 +0.8 3,335,143 - BT2676 0.27 -0.9 3,335,206 - BT2676 0.37 +2.0 3,335,240 - BT2676 0.43 +1.0 3,335,294 - BT2676 0.51 +0.2 3,335,294 - BT2676 0.51 -1.0 3,335,306 - BT2676 0.53 +0.6 3,335,326 + BT2676 0.56 +1.3 3,335,365 - BT2676 0.62 +2.1 3,335,412 + BT2676 0.70 -1.0 3,335,419 + BT2676 0.71 -1.6 3,335,538 - BT2676 0.90 -0.0 3,335,588 + +1.3 3,335,603 + -1.0 3,335,611 - -3.5 3,335,663 + -2.0 3,335,664 - -1.0 3,335,664 - -1.7 3,335,694 + -0.6 3,335,768 + -2.8 3,335,769 - -1.0 3,335,821 - -0.0 3,335,821 - -2.0 3,335,851 + -3.0 3,335,851 + -2.6 3,335,852 - -0.2
Or see this region's nucleotide sequence