Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2369 and BT2370 are separated by 197 nucleotides BT2370 and BT2371 are separated by 144 nucleotides BT2371 and BT2372 are separated by 226 nucleotides
BT2369: BT2369 - hypothetical protein (NCBI ptt file), at 2,955,211 to 2,955,840
BT2369
BT2370: BT2370 - hypothetical protein (NCBI ptt file), at 2,956,038 to 2,956,508
BT2370
BT2371: BT2371 - putative protease/amidase (NCBI ptt file), at 2,956,653 to 2,957,231
BT2371
BT2372: BT2372 - transcriptional regulator (NCBI ptt file), at 2,957,458 to 2,958,405
BT2372
Position (kb)
2956
2957
2958 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2955.781 kb on - strand at 2955.817 kb on + strand at 2955.853 kb on + strand at 2955.854 kb on - strand at 2955.883 kb on - strand at 2955.889 kb on + strand at 2955.890 kb on - strand at 2955.905 kb on + strand at 2955.910 kb on + strand at 2955.911 kb on - strand at 2955.923 kb on - strand at 2955.980 kb on - strand at 2955.981 kb on + strand at 2956.078 kb on - strand at 2956.079 kb on + strand at 2956.079 kb on + strand at 2956.086 kb on - strand, within BT2370 at 2956.087 kb on + strand, within BT2370 at 2956.088 kb on - strand, within BT2370 at 2956.106 kb on - strand, within BT2370 at 2956.161 kb on - strand, within BT2370 at 2956.323 kb on - strand, within BT2370 at 2956.397 kb on - strand, within BT2370 at 2956.404 kb on + strand, within BT2370 at 2956.404 kb on + strand, within BT2370 at 2956.405 kb on - strand, within BT2370 at 2956.459 kb on - strand, within BT2370 at 2956.463 kb on - strand at 2956.521 kb on + strand at 2956.521 kb on + strand at 2956.529 kb on - strand at 2956.529 kb on - strand at 2956.530 kb on + strand at 2956.530 kb on + strand at 2956.531 kb on - strand at 2956.546 kb on + strand at 2956.556 kb on - strand at 2956.603 kb on + strand at 2956.603 kb on + strand at 2956.612 kb on - strand at 2956.626 kb on - strand at 2956.751 kb on - strand, within BT2371 at 2956.751 kb on - strand, within BT2371 at 2956.761 kb on + strand, within BT2371 at 2956.761 kb on + strand, within BT2371 at 2956.763 kb on + strand, within BT2371 at 2956.797 kb on + strand, within BT2371 at 2956.849 kb on + strand, within BT2371 at 2956.886 kb on + strand, within BT2371 at 2956.906 kb on - strand, within BT2371 at 2956.976 kb on - strand, within BT2371 at 2957.033 kb on - strand, within BT2371 at 2957.064 kb on - strand, within BT2371 at 2957.084 kb on + strand, within BT2371 at 2957.085 kb on - strand, within BT2371 at 2957.208 kb on - strand at 2957.223 kb on + strand at 2957.267 kb on - strand at 2957.345 kb on + strand at 2957.345 kb on + strand at 2957.371 kb on - strand at 2957.403 kb on + strand at 2957.432 kb on - strand at 2957.463 kb on + strand at 2957.463 kb on + strand at 2957.464 kb on - strand at 2957.465 kb on + strand at 2957.465 kb on + strand at 2957.465 kb on + strand at 2957.466 kb on - strand at 2957.529 kb on - strand at 2957.619 kb on - strand, within BT2372 at 2957.688 kb on + strand, within BT2372 at 2957.718 kb on + strand, within BT2372 at 2957.738 kb on + strand, within BT2372 at 2957.838 kb on + strand, within BT2372 at 2957.867 kb on - strand, within BT2372 at 2957.886 kb on - strand, within BT2372 at 2957.889 kb on - strand, within BT2372 at 2957.889 kb on - strand, within BT2372 at 2957.992 kb on - strand, within BT2372 at 2957.992 kb on - strand, within BT2372 at 2958.033 kb on + strand, within BT2372 at 2958.062 kb on - strand, within BT2372 at 2958.126 kb on + strand, within BT2372 at 2958.147 kb on + strand, within BT2372 at 2958.147 kb on + strand, within BT2372 at 2958.148 kb on - strand, within BT2372 at 2958.214 kb on + strand, within BT2372 at 2958.215 kb on - strand, within BT2372
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 2,955,781 - -0.5 2,955,817 + -1.0 2,955,853 + -2.0 2,955,854 - -2.3 2,955,883 - -3.0 2,955,889 + -1.0 2,955,890 - -1.6 2,955,905 + -2.8 2,955,910 + +0.2 2,955,911 - -0.6 2,955,923 - -0.0 2,955,980 - -1.0 2,955,981 + -0.6 2,956,078 - -0.0 2,956,079 + -2.6 2,956,079 + +1.6 2,956,086 - BT2370 0.10 -0.9 2,956,087 + BT2370 0.10 -2.8 2,956,088 - BT2370 0.11 -0.0 2,956,106 - BT2370 0.14 +0.2 2,956,161 - BT2370 0.26 -2.0 2,956,323 - BT2370 0.61 -1.3 2,956,397 - BT2370 0.76 -2.7 2,956,404 + BT2370 0.78 -1.8 2,956,404 + BT2370 0.78 -0.3 2,956,405 - BT2370 0.78 +0.9 2,956,459 - BT2370 0.89 +0.6 2,956,463 - -0.6 2,956,521 + +1.6 2,956,521 + -0.7 2,956,529 - -0.8 2,956,529 - -0.9 2,956,530 + +1.4 2,956,530 + -0.8 2,956,531 - -0.5 2,956,546 + -0.1 2,956,556 - -0.0 2,956,603 + -1.4 2,956,603 + +2.5 2,956,612 - -1.6 2,956,626 - -0.8 2,956,751 - BT2371 0.17 -2.7 2,956,751 - BT2371 0.17 -1.6 2,956,761 + BT2371 0.19 -2.8 2,956,761 + BT2371 0.19 -0.0 2,956,763 + BT2371 0.19 -0.6 2,956,797 + BT2371 0.25 -1.5 2,956,849 + BT2371 0.34 -1.0 2,956,886 + BT2371 0.40 -3.8 2,956,906 - BT2371 0.44 -2.0 2,956,976 - BT2371 0.56 -0.9 2,957,033 - BT2371 0.66 -1.9 2,957,064 - BT2371 0.71 +0.6 2,957,084 + BT2371 0.74 -0.4 2,957,085 - BT2371 0.75 -0.9 2,957,208 - +2.3 2,957,223 + -0.2 2,957,267 - +0.6 2,957,345 + -0.6 2,957,345 + +0.6 2,957,371 - -2.0 2,957,403 + -1.0 2,957,432 - -1.9 2,957,463 + -0.3 2,957,463 + -0.0 2,957,464 - -1.3 2,957,465 + -1.7 2,957,465 + -1.6 2,957,465 + -2.8 2,957,466 - -0.7 2,957,529 - -1.7 2,957,619 - BT2372 0.17 +0.2 2,957,688 + BT2372 0.24 +1.6 2,957,718 + BT2372 0.27 -0.0 2,957,738 + BT2372 0.30 -1.0 2,957,838 + BT2372 0.40 +0.9 2,957,867 - BT2372 0.43 -0.6 2,957,886 - BT2372 0.45 -0.0 2,957,889 - BT2372 0.45 -0.1 2,957,889 - BT2372 0.45 -0.0 2,957,992 - BT2372 0.56 -0.7 2,957,992 - BT2372 0.56 +1.9 2,958,033 + BT2372 0.61 +2.3 2,958,062 - BT2372 0.64 -0.8 2,958,126 + BT2372 0.70 -1.0 2,958,147 + BT2372 0.73 -1.8 2,958,147 + BT2372 0.73 -0.0 2,958,148 - BT2372 0.73 -0.0 2,958,214 + BT2372 0.80 +2.1 2,958,215 - BT2372 0.80 +2.2
Or see this region's nucleotide sequence