Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2036 and BT2037 are separated by 298 nucleotides BT2037 and BT2038 are separated by 86 nucleotides BT2038 and BT2039 are separated by 129 nucleotides
BT2036: BT2036 - conserved hypothetical protein (NCBI ptt file), at 2,556,012 to 2,556,593
BT2036
BT2037: BT2037 - hypothetical protein (NCBI ptt file), at 2,556,892 to 2,557,302
BT2037
BT2038: BT2038 - cation efflux system protein (NCBI ptt file), at 2,557,389 to 2,558,660
BT2038
BT2039: BT2039 - cation efflux system protein, AcrB/AcrD/AcrF family protein (NCBI ptt file), at 2,558,790 to 2,561,918
BT2039
Position (kb)
2557
2558
2559 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2556.466 kb on + strand, within BT2036 at 2556.466 kb on + strand, within BT2036 at 2556.466 kb on + strand, within BT2036 at 2556.479 kb on - strand, within BT2036 at 2556.549 kb on + strand at 2556.794 kb on - strand at 2556.831 kb on - strand at 2556.832 kb on + strand at 2556.848 kb on + strand at 2556.879 kb on - strand at 2556.882 kb on + strand at 2556.882 kb on + strand at 2556.917 kb on + strand at 2556.917 kb on + strand at 2556.918 kb on - strand at 2556.926 kb on + strand at 2556.927 kb on - strand at 2556.934 kb on - strand, within BT2037 at 2556.945 kb on + strand, within BT2037 at 2556.946 kb on - strand, within BT2037 at 2556.963 kb on + strand, within BT2037 at 2557.219 kb on + strand, within BT2037 at 2557.261 kb on - strand, within BT2037 at 2557.339 kb on + strand at 2557.371 kb on - strand at 2557.389 kb on - strand at 2557.401 kb on - strand at 2557.455 kb on - strand at 2557.697 kb on + strand, within BT2038 at 2557.697 kb on + strand, within BT2038 at 2557.698 kb on - strand, within BT2038 at 2557.745 kb on - strand, within BT2038 at 2557.745 kb on - strand, within BT2038 at 2557.860 kb on - strand, within BT2038 at 2557.895 kb on - strand, within BT2038 at 2557.915 kb on - strand, within BT2038 at 2557.915 kb on - strand, within BT2038 at 2557.936 kb on - strand, within BT2038 at 2557.936 kb on - strand, within BT2038 at 2558.124 kb on + strand, within BT2038 at 2558.128 kb on + strand, within BT2038 at 2558.251 kb on - strand, within BT2038 at 2558.256 kb on - strand, within BT2038 at 2558.479 kb on - strand, within BT2038 at 2558.807 kb on - strand at 2558.807 kb on - strand at 2558.862 kb on - strand at 2558.874 kb on + strand at 2558.875 kb on - strand at 2558.875 kb on - strand at 2558.897 kb on - strand at 2559.095 kb on + strand at 2559.095 kb on + strand at 2559.096 kb on - strand at 2559.096 kb on - strand at 2559.097 kb on + strand at 2559.097 kb on + strand at 2559.098 kb on - strand at 2559.099 kb on + strand at 2559.100 kb on - strand at 2559.100 kb on - strand at 2559.471 kb on + strand, within BT2039 at 2559.472 kb on - strand, within BT2039 at 2559.474 kb on - strand, within BT2039 at 2559.584 kb on - strand, within BT2039
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 2,556,466 + BT2036 0.78 +1.0 2,556,466 + BT2036 0.78 -0.1 2,556,466 + BT2036 0.78 +0.4 2,556,479 - BT2036 0.80 -1.5 2,556,549 + +1.0 2,556,794 - +2.0 2,556,831 - -2.5 2,556,832 + -1.8 2,556,848 + -1.0 2,556,879 - -0.5 2,556,882 + -0.5 2,556,882 + -2.1 2,556,917 + +0.0 2,556,917 + +2.2 2,556,918 - -2.5 2,556,926 + -0.9 2,556,927 - -0.2 2,556,934 - BT2037 0.10 +0.6 2,556,945 + BT2037 0.13 -2.6 2,556,946 - BT2037 0.13 -2.5 2,556,963 + BT2037 0.17 -1.3 2,557,219 + BT2037 0.80 -2.0 2,557,261 - BT2037 0.90 +0.6 2,557,339 + -2.1 2,557,371 - -0.4 2,557,389 - +2.0 2,557,401 - -0.5 2,557,455 - +0.0 2,557,697 + BT2038 0.24 -2.8 2,557,697 + BT2038 0.24 -2.3 2,557,698 - BT2038 0.24 +0.6 2,557,745 - BT2038 0.28 -2.3 2,557,745 - BT2038 0.28 -1.5 2,557,860 - BT2038 0.37 -1.5 2,557,895 - BT2038 0.40 -1.9 2,557,915 - BT2038 0.41 -2.3 2,557,915 - BT2038 0.41 -1.8 2,557,936 - BT2038 0.43 -0.7 2,557,936 - BT2038 0.43 +0.0 2,558,124 + BT2038 0.58 -2.5 2,558,128 + BT2038 0.58 -2.3 2,558,251 - BT2038 0.68 -3.7 2,558,256 - BT2038 0.68 -1.0 2,558,479 - BT2038 0.86 +0.0 2,558,807 - -0.6 2,558,807 - +1.0 2,558,862 - -1.0 2,558,874 + -0.3 2,558,875 - -2.8 2,558,875 - -2.1 2,558,897 - -1.0 2,559,095 + +1.0 2,559,095 + +1.0 2,559,096 - -2.5 2,559,096 - +2.4 2,559,097 + +1.3 2,559,097 + +0.2 2,559,098 - -2.5 2,559,099 + -2.1 2,559,100 - -1.0 2,559,100 - -0.5 2,559,471 + BT2039 0.22 -1.0 2,559,472 - BT2039 0.22 -1.0 2,559,474 - BT2039 0.22 -2.0 2,559,584 - BT2039 0.25 -1.0
Or see this region's nucleotide sequence