Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0864 and BT0865 are separated by 215 nucleotides BT0865 and BT0866 are separated by 30 nucleotides
BT0864: BT0864 - putative permease (NCBI ptt file), at 1,059,288 to 1,061,645
BT0864
BT0865: BT0865 - putative chitobiase (NCBI ptt file), at 1,061,861 to 1,063,183
BT0865
BT0866: BT0866 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 1,063,214 to 1,065,112
BT0866
Position (kb)
1061
1062
1063
1064 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1060.919 kb on + strand, within BT0864 at 1061.115 kb on + strand, within BT0864 at 1061.131 kb on + strand, within BT0864 at 1061.156 kb on - strand, within BT0864 at 1061.156 kb on - strand, within BT0864 at 1061.232 kb on + strand, within BT0864 at 1061.235 kb on - strand, within BT0864 at 1061.235 kb on - strand, within BT0864 at 1061.236 kb on + strand, within BT0864 at 1061.289 kb on - strand, within BT0864 at 1061.336 kb on + strand, within BT0864 at 1061.375 kb on + strand, within BT0864 at 1061.375 kb on + strand, within BT0864 at 1061.434 kb on - strand at 1061.437 kb on - strand at 1061.473 kb on + strand at 1061.474 kb on - strand at 1061.542 kb on + strand at 1061.650 kb on + strand at 1061.656 kb on + strand at 1061.656 kb on + strand at 1061.657 kb on - strand at 1061.813 kb on - strand at 1061.818 kb on - strand at 1061.818 kb on - strand at 1061.869 kb on + strand at 1061.976 kb on + strand at 1062.116 kb on + strand, within BT0865 at 1062.122 kb on + strand, within BT0865 at 1062.141 kb on - strand, within BT0865 at 1062.522 kb on + strand, within BT0865 at 1062.543 kb on + strand, within BT0865 at 1062.544 kb on - strand, within BT0865 at 1062.551 kb on + strand, within BT0865 at 1062.552 kb on - strand, within BT0865 at 1062.635 kb on + strand, within BT0865 at 1062.650 kb on - strand, within BT0865 at 1062.659 kb on + strand, within BT0865 at 1062.666 kb on - strand, within BT0865 at 1062.672 kb on - strand, within BT0865 at 1063.161 kb on - strand at 1063.163 kb on + strand at 1063.164 kb on - strand at 1063.183 kb on + strand at 1063.207 kb on + strand at 1063.208 kb on - strand at 1063.209 kb on + strand at 1063.319 kb on + strand at 1063.530 kb on - strand, within BT0866 at 1063.732 kb on + strand, within BT0866 at 1063.745 kb on - strand, within BT0866 at 1063.845 kb on + strand, within BT0866
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 1,060,919 + BT0864 0.69 -2.0 1,061,115 + BT0864 0.77 +0.0 1,061,131 + BT0864 0.78 +0.5 1,061,156 - BT0864 0.79 +2.8 1,061,156 - BT0864 0.79 +2.4 1,061,232 + BT0864 0.82 -2.5 1,061,235 - BT0864 0.83 -0.1 1,061,235 - BT0864 0.83 +0.8 1,061,236 + BT0864 0.83 -2.2 1,061,289 - BT0864 0.85 -1.5 1,061,336 + BT0864 0.87 -2.8 1,061,375 + BT0864 0.89 -2.2 1,061,375 + BT0864 0.89 -3.0 1,061,434 - -0.2 1,061,437 - +2.6 1,061,473 + +1.9 1,061,474 - +1.4 1,061,542 + -0.6 1,061,650 + -1.8 1,061,656 + -0.4 1,061,656 + -2.2 1,061,657 - -1.1 1,061,813 - +0.9 1,061,818 - -0.7 1,061,818 - -0.2 1,061,869 + -0.9 1,061,976 + -1.2 1,062,116 + BT0865 0.19 +3.2 1,062,122 + BT0865 0.20 -0.5 1,062,141 - BT0865 0.21 +0.8 1,062,522 + BT0865 0.50 -1.2 1,062,543 + BT0865 0.52 -3.0 1,062,544 - BT0865 0.52 -1.4 1,062,551 + BT0865 0.52 -1.2 1,062,552 - BT0865 0.52 -2.8 1,062,635 + BT0865 0.59 -3.8 1,062,650 - BT0865 0.60 -3.4 1,062,659 + BT0865 0.60 -2.7 1,062,666 - BT0865 0.61 +1.8 1,062,672 - BT0865 0.61 -0.2 1,063,161 - -1.2 1,063,163 + +1.8 1,063,164 - -0.5 1,063,183 + -1.8 1,063,207 + -0.2 1,063,208 - -2.8 1,063,209 + -0.2 1,063,319 + -2.2 1,063,530 - BT0866 0.17 -0.2 1,063,732 + BT0866 0.27 -0.8 1,063,745 - BT0866 0.28 +2.1 1,063,845 + BT0866 0.33 -1.6
Or see this region's nucleotide sequence