Experiment: BHIS with Chlorpromazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0108 and BT0109 overlap by 38 nucleotides BT0109 and BT0110 are separated by 210 nucleotides BT0110 and BT0111 are separated by 167 nucleotides BT0111 and BT0112 overlap by 23 nucleotides
BT0108: BT0108 - conserved hypothetical protein (NCBI ptt file), at 100,303 to 102,303
BT0108
BT0109: BT0109 - conserved hypothetical protein (NCBI ptt file), at 102,266 to 102,625
BT0109
BT0110: BT0110 - putative regulatory protein (NCBI ptt file), at 102,836 to 103,696
BT0110
BT0111: BT0111 - hypothetical protein (NCBI ptt file), at 103,864 to 104,115
BT0111
BT0112: BT0112 - putative permease (NCBI ptt file), at 104,093 to 104,998
BT0112
Position (kb) 102
103
104 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 101.853 kb on - strand, within BT0108 at 101.864 kb on + strand, within BT0108 at 101.941 kb on + strand, within BT0108 at 101.943 kb on + strand, within BT0108 at 102.142 kb on + strand at 102.232 kb on - strand at 102.242 kb on + strand at 102.306 kb on - strand, within BT0109 at 102.526 kb on + strand, within BT0109 at 102.551 kb on + strand, within BT0109 at 102.617 kb on + strand at 102.728 kb on + strand at 102.839 kb on + strand at 102.889 kb on + strand at 102.923 kb on + strand, within BT0110 at 102.923 kb on + strand, within BT0110 at 102.924 kb on - strand, within BT0110 at 102.932 kb on - strand, within BT0110 at 102.943 kb on - strand, within BT0110 at 102.978 kb on - strand, within BT0110 at 102.982 kb on - strand, within BT0110 at 102.989 kb on - strand, within BT0110 at 103.000 kb on + strand, within BT0110 at 103.036 kb on - strand, within BT0110 at 103.056 kb on - strand, within BT0110 at 103.058 kb on + strand, within BT0110 at 103.065 kb on - strand, within BT0110 at 103.143 kb on + strand, within BT0110 at 103.144 kb on - strand, within BT0110 at 103.171 kb on + strand, within BT0110 at 103.212 kb on + strand, within BT0110 at 103.326 kb on + strand, within BT0110 at 103.390 kb on - strand, within BT0110 at 103.519 kb on + strand, within BT0110 at 103.599 kb on - strand, within BT0110 at 103.605 kb on - strand, within BT0110 at 103.702 kb on - strand at 103.730 kb on - strand at 103.794 kb on + strand at 103.794 kb on + strand at 103.803 kb on + strand at 103.821 kb on + strand at 103.824 kb on + strand at 103.998 kb on + strand, within BT0111 at 104.004 kb on + strand, within BT0111 at 104.111 kb on + strand at 104.116 kb on - strand at 104.163 kb on - strand at 104.163 kb on - strand at 104.261 kb on - strand, within BT0112 at 104.293 kb on + strand, within BT0112 at 104.399 kb on - strand, within BT0112 at 104.401 kb on - strand, within BT0112 at 104.442 kb on + strand, within BT0112 at 104.444 kb on + strand, within BT0112 at 104.458 kb on + strand, within BT0112 at 104.477 kb on + strand, within BT0112 at 104.666 kb on - strand, within BT0112 at 104.690 kb on - strand, within BT0112 at 104.693 kb on + strand, within BT0112 at 104.695 kb on + strand, within BT0112 at 104.695 kb on + strand, within BT0112
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Chlorpromazine 0.1 mM remove 101,853 - BT0108 0.77 -3.2 101,864 + BT0108 0.78 -0.0 101,941 + BT0108 0.82 -0.0 101,943 + BT0108 0.82 -2.4 102,142 + +0.6 102,232 - +0.7 102,242 + +1.5 102,306 - BT0109 0.11 +1.5 102,526 + BT0109 0.72 -2.8 102,551 + BT0109 0.79 -2.2 102,617 + -1.2 102,728 + +1.0 102,839 + -3.0 102,889 + -0.0 102,923 + BT0110 0.10 -1.0 102,923 + BT0110 0.10 +0.3 102,924 - BT0110 0.10 -0.8 102,932 - BT0110 0.11 -0.0 102,943 - BT0110 0.12 -1.0 102,978 - BT0110 0.16 -0.4 102,982 - BT0110 0.17 -0.5 102,989 - BT0110 0.18 -0.5 103,000 + BT0110 0.19 +0.7 103,036 - BT0110 0.23 +1.4 103,056 - BT0110 0.26 -0.6 103,058 + BT0110 0.26 +0.1 103,065 - BT0110 0.27 +1.5 103,143 + BT0110 0.36 -2.7 103,144 - BT0110 0.36 +2.8 103,171 + BT0110 0.39 +1.3 103,212 + BT0110 0.44 -1.6 103,326 + BT0110 0.57 +3.5 103,390 - BT0110 0.64 +2.6 103,519 + BT0110 0.79 +0.4 103,599 - BT0110 0.89 -2.0 103,605 - BT0110 0.89 +0.1 103,702 - -0.4 103,730 - +1.5 103,794 + +0.7 103,794 + +0.3 103,803 + -0.8 103,821 + -2.3 103,824 + -1.0 103,998 + BT0111 0.53 +0.3 104,004 + BT0111 0.56 +0.5 104,111 + +2.5 104,116 - +1.5 104,163 - -0.0 104,163 - -1.1 104,261 - BT0112 0.19 -1.0 104,293 + BT0112 0.22 -3.2 104,399 - BT0112 0.34 -1.0 104,401 - BT0112 0.34 -1.0 104,442 + BT0112 0.39 -1.0 104,444 + BT0112 0.39 -1.1 104,458 + BT0112 0.40 -0.0 104,477 + BT0112 0.42 +1.0 104,666 - BT0112 0.63 -1.0 104,690 - BT0112 0.66 -1.4 104,693 + BT0112 0.66 -1.7 104,695 + BT0112 0.66 -1.4 104,695 + BT0112 0.66 -0.0
Or see this region's nucleotide sequence