Experiment: MoLS4 with L-Isoleucine as nutrient 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livH and DVU2744 are separated by 109 nucleotides DVU2744 and DVU2746 are separated by 377 nucleotides
DVU2743: livH - high-affinity branched-chain amino acid ABC ransporter, permease protein (TIGR), at 2,848,524 to 2,849,444
livH
DVU2744: DVU2744 - high-affinity branched-chain amino acid ABC transporter, perisplasmic amino acid binding protein (TIGR), at 2,849,554 to 2,850,711
DVU2744
DVU2746: DVU2746 - conserved hypothetical protein (TIGR), at 2,851,089 to 2,852,033
DVU2746
Position (kb)
2849
2850
2851 Strain fitness (log2 ratio)
-2
-1
0
1 at 2848.556 kb on + strand at 2848.564 kb on - strand at 2848.587 kb on - strand at 2848.587 kb on - strand at 2848.623 kb on + strand, within livH at 2848.768 kb on + strand, within livH at 2848.768 kb on + strand, within livH at 2848.768 kb on + strand, within livH at 2848.776 kb on - strand, within livH at 2848.776 kb on - strand, within livH at 2848.780 kb on + strand, within livH at 2848.788 kb on - strand, within livH at 2848.835 kb on - strand, within livH at 2848.848 kb on - strand, within livH at 2848.932 kb on + strand, within livH at 2849.002 kb on + strand, within livH at 2849.019 kb on - strand, within livH at 2849.145 kb on + strand, within livH at 2849.223 kb on + strand, within livH at 2849.230 kb on - strand, within livH at 2849.284 kb on - strand, within livH at 2849.322 kb on - strand, within livH at 2849.355 kb on + strand at 2849.356 kb on + strand at 2849.356 kb on + strand at 2849.356 kb on - strand at 2849.368 kb on + strand at 2849.550 kb on + strand at 2849.766 kb on - strand, within DVU2744 at 2849.780 kb on + strand, within DVU2744 at 2849.828 kb on + strand, within DVU2744 at 2849.888 kb on + strand, within DVU2744 at 2849.893 kb on - strand, within DVU2744 at 2849.903 kb on + strand, within DVU2744 at 2849.912 kb on + strand, within DVU2744 at 2849.971 kb on + strand, within DVU2744 at 2850.046 kb on + strand, within DVU2744 at 2850.061 kb on - strand, within DVU2744 at 2850.080 kb on + strand, within DVU2744 at 2850.085 kb on + strand, within DVU2744 at 2850.123 kb on - strand, within DVU2744 at 2850.149 kb on - strand, within DVU2744 at 2850.271 kb on - strand, within DVU2744 at 2850.292 kb on + strand, within DVU2744 at 2850.361 kb on - strand, within DVU2744 at 2850.481 kb on - strand, within DVU2744 at 2850.536 kb on - strand, within DVU2744 at 2850.536 kb on - strand, within DVU2744 at 2850.616 kb on - strand at 2850.668 kb on + strand at 2851.140 kb on - strand at 2851.204 kb on + strand, within DVU2746 at 2851.204 kb on + strand, within DVU2746 at 2851.371 kb on - strand, within DVU2746 at 2851.398 kb on + strand, within DVU2746 at 2851.404 kb on - strand, within DVU2746 at 2851.406 kb on + strand, within DVU2746 at 2851.418 kb on + strand, within DVU2746 at 2851.422 kb on + strand, within DVU2746 at 2851.444 kb on + strand, within DVU2746 at 2851.453 kb on + strand, within DVU2746 at 2851.461 kb on - strand, within DVU2746 at 2851.461 kb on - strand, within DVU2746 at 2851.497 kb on + strand, within DVU2746 at 2851.504 kb on + strand, within DVU2746 at 2851.505 kb on - strand, within DVU2746 at 2851.505 kb on - strand, within DVU2746 at 2851.551 kb on - strand, within DVU2746 at 2851.556 kb on + strand, within DVU2746 at 2851.556 kb on + strand, within DVU2746 at 2851.556 kb on + strand, within DVU2746 at 2851.564 kb on - strand, within DVU2746 at 2851.564 kb on - strand, within DVU2746 at 2851.619 kb on + strand, within DVU2746 at 2851.621 kb on - strand, within DVU2746 at 2851.636 kb on + strand, within DVU2746 at 2851.689 kb on + strand, within DVU2746 at 2851.697 kb on - strand, within DVU2746
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Isoleucine as nutrient 10mM remove 2,848,556 + -0.1 2,848,564 - +1.7 2,848,587 - +0.1 2,848,587 - +0.0 2,848,623 + livH DVU2743 0.11 +0.1 2,848,768 + livH DVU2743 0.26 -0.2 2,848,768 + livH DVU2743 0.26 -0.1 2,848,768 + livH DVU2743 0.26 -0.3 2,848,776 - livH DVU2743 0.27 +0.2 2,848,776 - livH DVU2743 0.27 +0.8 2,848,780 + livH DVU2743 0.28 -0.1 2,848,788 - livH DVU2743 0.29 +1.6 2,848,835 - livH DVU2743 0.34 +0.1 2,848,848 - livH DVU2743 0.35 -0.4 2,848,932 + livH DVU2743 0.44 -0.0 2,849,002 + livH DVU2743 0.52 -0.0 2,849,019 - livH DVU2743 0.54 -0.2 2,849,145 + livH DVU2743 0.67 -0.1 2,849,223 + livH DVU2743 0.76 -0.0 2,849,230 - livH DVU2743 0.77 -0.2 2,849,284 - livH DVU2743 0.83 +0.2 2,849,322 - livH DVU2743 0.87 +0.4 2,849,355 + -0.3 2,849,356 + -0.1 2,849,356 + +0.4 2,849,356 - -0.2 2,849,368 + -0.6 2,849,550 + -0.7 2,849,766 - DVU2744 0.18 -0.5 2,849,780 + DVU2744 0.20 +0.6 2,849,828 + DVU2744 0.24 -0.1 2,849,888 + DVU2744 0.29 -0.2 2,849,893 - DVU2744 0.29 -0.9 2,849,903 + DVU2744 0.30 -0.3 2,849,912 + DVU2744 0.31 +0.3 2,849,971 + DVU2744 0.36 +1.1 2,850,046 + DVU2744 0.42 -0.2 2,850,061 - DVU2744 0.44 -0.1 2,850,080 + DVU2744 0.45 -0.3 2,850,085 + DVU2744 0.46 -0.5 2,850,123 - DVU2744 0.49 +0.1 2,850,149 - DVU2744 0.51 -0.5 2,850,271 - DVU2744 0.62 +0.1 2,850,292 + DVU2744 0.64 +0.3 2,850,361 - DVU2744 0.70 -0.5 2,850,481 - DVU2744 0.80 +0.0 2,850,536 - DVU2744 0.85 -0.3 2,850,536 - DVU2744 0.85 +0.1 2,850,616 - +0.1 2,850,668 + -0.2 2,851,140 - +0.2 2,851,204 + DVU2746 0.12 -1.4 2,851,204 + DVU2746 0.12 -0.0 2,851,371 - DVU2746 0.30 +0.9 2,851,398 + DVU2746 0.33 -0.2 2,851,404 - DVU2746 0.33 -0.2 2,851,406 + DVU2746 0.34 -1.7 2,851,418 + DVU2746 0.35 -0.6 2,851,422 + DVU2746 0.35 -0.3 2,851,444 + DVU2746 0.38 +0.1 2,851,453 + DVU2746 0.39 -0.3 2,851,461 - DVU2746 0.39 +0.0 2,851,461 - DVU2746 0.39 -0.9 2,851,497 + DVU2746 0.43 -0.0 2,851,504 + DVU2746 0.44 -0.6 2,851,505 - DVU2746 0.44 -0.3 2,851,505 - DVU2746 0.44 -0.3 2,851,551 - DVU2746 0.49 -1.8 2,851,556 + DVU2746 0.49 -0.2 2,851,556 + DVU2746 0.49 +0.1 2,851,556 + DVU2746 0.49 +0.1 2,851,564 - DVU2746 0.50 -0.3 2,851,564 - DVU2746 0.50 -0.2 2,851,619 + DVU2746 0.56 -0.4 2,851,621 - DVU2746 0.56 -0.2 2,851,636 + DVU2746 0.58 -1.4 2,851,689 + DVU2746 0.63 +0.2 2,851,697 - DVU2746 0.64 -0.3
Or see this region's nucleotide sequence