Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2817 and Psyr_2818 are separated by 20 nucleotides Psyr_2818 and Psyr_2819 overlap by 4 nucleotides Psyr_2819 and Psyr_2820 are separated by 329 nucleotides Psyr_2820 and Psyr_2821 overlap by 4 nucleotides
Psyr_2817: Psyr_2817 - hypothetical protein, at 3,392,815 to 3,393,282
_2817
Psyr_2818: Psyr_2818 - hypothetical protein, at 3,393,303 to 3,393,710
_2818
Psyr_2819: Psyr_2819 - hypothetical protein, at 3,393,707 to 3,393,898
_2819
Psyr_2820: Psyr_2820 - RecT protein, at 3,394,228 to 3,395,115
_2820
Psyr_2821: Psyr_2821 - conserved hypothetical protein, at 3,395,112 to 3,396,773
_2821
Position (kb) 3394
3395
3396 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.264 kb on + strand at 3393.265 kb on - strand at 3393.265 kb on - strand at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.549 kb on + strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.550 kb on - strand, within Psyr_2818 at 3393.980 kb on - strand at 3394.092 kb on + strand at 3394.092 kb on + strand at 3394.092 kb on + strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.093 kb on - strand at 3394.177 kb on - strand at 3394.227 kb on + strand at 3394.227 kb on + strand at 3394.228 kb on - strand at 3394.316 kb on + strand at 3394.534 kb on - strand, within Psyr_2820 at 3394.831 kb on + strand, within Psyr_2820 at 3394.832 kb on - strand, within Psyr_2820 at 3394.945 kb on + strand, within Psyr_2820 at 3395.047 kb on + strand at 3395.048 kb on - strand at 3395.048 kb on - strand at 3395.123 kb on + strand at 3395.169 kb on + strand at 3395.170 kb on - strand at 3395.289 kb on + strand, within Psyr_2821 at 3395.289 kb on + strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.502 kb on + strand, within Psyr_2821 at 3395.502 kb on + strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.505 kb on + strand, within Psyr_2821 at 3395.505 kb on + strand, within Psyr_2821 at 3395.602 kb on - strand, within Psyr_2821 at 3395.674 kb on - strand, within Psyr_2821 at 3395.747 kb on - strand, within Psyr_2821
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 3,393,264 + -1.3 3,393,264 + -0.9 3,393,264 + +0.4 3,393,264 + -0.7 3,393,264 + +0.2 3,393,264 + -0.5 3,393,264 + -0.6 3,393,264 + -2.4 3,393,265 - +0.0 3,393,265 - -0.4 3,393,549 + Psyr_2818 0.60 -3.4 3,393,549 + Psyr_2818 0.60 +0.5 3,393,549 + Psyr_2818 0.60 -0.5 3,393,549 + Psyr_2818 0.60 -0.5 3,393,549 + Psyr_2818 0.60 +0.0 3,393,549 + Psyr_2818 0.60 +0.3 3,393,549 + Psyr_2818 0.60 +1.0 3,393,550 - Psyr_2818 0.61 -0.1 3,393,550 - Psyr_2818 0.61 -2.0 3,393,550 - Psyr_2818 0.61 +0.0 3,393,550 - Psyr_2818 0.61 -0.7 3,393,980 - +0.8 3,394,092 + -0.5 3,394,092 + +2.3 3,394,092 + -0.0 3,394,093 - -0.2 3,394,093 - +0.0 3,394,093 - -0.6 3,394,093 - -1.0 3,394,177 - -1.5 3,394,227 + -1.8 3,394,227 + -0.9 3,394,228 - +1.5 3,394,316 + -0.2 3,394,534 - Psyr_2820 0.34 -0.2 3,394,831 + Psyr_2820 0.68 +1.0 3,394,832 - Psyr_2820 0.68 -0.2 3,394,945 + Psyr_2820 0.81 +1.5 3,395,047 + -1.0 3,395,048 - -2.3 3,395,048 - -0.4 3,395,123 + +0.4 3,395,169 + +2.6 3,395,170 - +0.7 3,395,289 + Psyr_2821 0.11 +0.1 3,395,289 + Psyr_2821 0.11 -1.0 3,395,290 - Psyr_2821 0.11 +0.1 3,395,290 - Psyr_2821 0.11 +1.2 3,395,290 - Psyr_2821 0.11 -0.4 3,395,290 - Psyr_2821 0.11 +2.2 3,395,290 - Psyr_2821 0.11 -2.2 3,395,290 - Psyr_2821 0.11 -0.9 3,395,502 + Psyr_2821 0.23 +1.4 3,395,502 + Psyr_2821 0.23 -0.1 3,395,503 - Psyr_2821 0.24 -1.7 3,395,503 - Psyr_2821 0.24 +2.0 3,395,503 - Psyr_2821 0.24 +0.6 3,395,505 + Psyr_2821 0.24 +2.6 3,395,505 + Psyr_2821 0.24 -0.6 3,395,602 - Psyr_2821 0.29 +0.6 3,395,674 - Psyr_2821 0.34 -0.6 3,395,747 - Psyr_2821 0.38 -0.1
Or see this region's nucleotide sequence