Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2271 and Psyr_2272 are separated by 48 nucleotides Psyr_2272 and Psyr_2273 are separated by 288 nucleotides
Psyr_2271: Psyr_2271 - membrane protein, putative, at 2,636,708 to 2,637,874
_2271
Psyr_2272: Psyr_2272 - Helix-turn-helix motif protein, at 2,637,923 to 2,638,528
_2272
Psyr_2273: Psyr_2273 - L-glutamine synthetase, at 2,638,817 to 2,640,151
_2273
Position (kb)
2637
2638
2639 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2636.963 kb on + strand, within Psyr_2271 at 2636.964 kb on - strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.080 kb on + strand, within Psyr_2271 at 2637.081 kb on - strand, within Psyr_2271 at 2637.081 kb on - strand, within Psyr_2271 at 2637.298 kb on + strand, within Psyr_2271 at 2637.299 kb on - strand, within Psyr_2271 at 2637.636 kb on + strand, within Psyr_2271 at 2637.637 kb on - strand, within Psyr_2271 at 2637.637 kb on - strand, within Psyr_2271 at 2637.639 kb on + strand, within Psyr_2271 at 2637.680 kb on + strand, within Psyr_2271 at 2637.680 kb on + strand, within Psyr_2271 at 2637.680 kb on + strand, within Psyr_2271 at 2637.680 kb on + strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.681 kb on - strand, within Psyr_2271 at 2637.741 kb on - strand, within Psyr_2271 at 2637.786 kb on + strand at 2637.866 kb on + strand at 2637.866 kb on + strand at 2637.873 kb on - strand at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.158 kb on + strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.159 kb on - strand, within Psyr_2272 at 2638.207 kb on - strand, within Psyr_2272 at 2638.294 kb on - strand, within Psyr_2272 at 2638.483 kb on - strand at 2638.483 kb on - strand at 2638.617 kb on - strand at 2638.646 kb on + strand at 2638.647 kb on - strand at 2638.647 kb on - strand at 2638.647 kb on - strand at 2638.692 kb on - strand at 2638.749 kb on - strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.865 kb on + strand at 2638.866 kb on - strand at 2638.869 kb on + strand at 2638.870 kb on - strand at 2639.127 kb on - strand, within Psyr_2273 at 2639.279 kb on + strand, within Psyr_2273 at 2639.280 kb on - strand, within Psyr_2273 at 2639.355 kb on - strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.399 kb on + strand, within Psyr_2273 at 2639.400 kb on - strand, within Psyr_2273 at 2639.400 kb on - strand, within Psyr_2273
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 2,636,963 + Psyr_2271 0.22 -0.4 2,636,964 - Psyr_2271 0.22 +0.9 2,637,080 + Psyr_2271 0.32 -2.8 2,637,080 + Psyr_2271 0.32 -0.5 2,637,080 + Psyr_2271 0.32 +0.1 2,637,080 + Psyr_2271 0.32 -0.1 2,637,081 - Psyr_2271 0.32 -0.5 2,637,081 - Psyr_2271 0.32 -1.7 2,637,298 + Psyr_2271 0.51 +1.1 2,637,299 - Psyr_2271 0.51 -0.8 2,637,636 + Psyr_2271 0.80 -1.2 2,637,637 - Psyr_2271 0.80 +0.5 2,637,637 - Psyr_2271 0.80 -0.9 2,637,639 + Psyr_2271 0.80 -0.3 2,637,680 + Psyr_2271 0.83 +0.2 2,637,680 + Psyr_2271 0.83 -1.5 2,637,680 + Psyr_2271 0.83 +1.4 2,637,680 + Psyr_2271 0.83 +0.5 2,637,681 - Psyr_2271 0.83 -0.9 2,637,681 - Psyr_2271 0.83 -0.3 2,637,681 - Psyr_2271 0.83 +0.9 2,637,681 - Psyr_2271 0.83 +0.9 2,637,741 - Psyr_2271 0.89 -0.9 2,637,786 + -0.5 2,637,866 + +0.7 2,637,866 + +0.3 2,637,873 - -0.6 2,638,158 + Psyr_2272 0.39 +1.2 2,638,158 + Psyr_2272 0.39 +0.4 2,638,158 + Psyr_2272 0.39 +0.1 2,638,158 + Psyr_2272 0.39 -3.7 2,638,158 + Psyr_2272 0.39 +0.3 2,638,159 - Psyr_2272 0.39 +0.9 2,638,159 - Psyr_2272 0.39 +0.1 2,638,159 - Psyr_2272 0.39 +0.9 2,638,159 - Psyr_2272 0.39 +0.7 2,638,207 - Psyr_2272 0.47 +2.3 2,638,294 - Psyr_2272 0.61 +0.5 2,638,483 - +1.5 2,638,483 - +0.8 2,638,617 - -2.0 2,638,646 + -1.1 2,638,647 - -1.8 2,638,647 - -0.9 2,638,647 - -0.9 2,638,692 - -0.3 2,638,749 - +0.1 2,638,865 + -0.3 2,638,865 + -0.9 2,638,865 + +2.5 2,638,865 + +3.3 2,638,865 + +1.5 2,638,865 + +0.6 2,638,866 - -0.3 2,638,869 + -1.2 2,638,870 - +0.1 2,639,127 - Psyr_2273 0.23 -0.1 2,639,279 + Psyr_2273 0.35 +1.4 2,639,280 - Psyr_2273 0.35 -0.2 2,639,355 - Psyr_2273 0.40 -0.5 2,639,399 + Psyr_2273 0.44 -0.4 2,639,399 + Psyr_2273 0.44 -0.2 2,639,399 + Psyr_2273 0.44 -0.5 2,639,399 + Psyr_2273 0.44 +1.6 2,639,399 + Psyr_2273 0.44 +0.4 2,639,399 + Psyr_2273 0.44 +1.5 2,639,399 + Psyr_2273 0.44 -0.4 2,639,400 - Psyr_2273 0.44 +0.6 2,639,400 - Psyr_2273 0.44 -1.6
Or see this region's nucleotide sequence