Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2220 and Psyr_2221 are separated by 214 nucleotides Psyr_2221 and Psyr_2222 are separated by 3 nucleotides Psyr_2222 and Psyr_2223 overlap by 4 nucleotides Psyr_2223 and Psyr_2224 are separated by 13 nucleotides
Psyr_2220: Psyr_2220 - Histidine kinase, HAMP region:Cache:Bacterial chemotaxis sensory transducer, at 2,579,677 to 2,581,617
_2220
Psyr_2221: Psyr_2221 - sarcosine oxidase, gamma subunit, at 2,581,832 to 2,582,404
_2221
Psyr_2222: Psyr_2222 - aminomethyltransferase, at 2,582,408 to 2,585,314
_2222
Psyr_2223: Psyr_2223 - sarcosine oxidase, delta subunit, at 2,585,311 to 2,585,616
_2223
Psyr_2224: Psyr_2224 - FAD dependent oxidoreductase, at 2,585,630 to 2,586,871
_2224
Position (kb)
2582
2583
2584
2585
2586 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2581.421 kb on + strand, within Psyr_2220 at 2581.421 kb on + strand, within Psyr_2220 at 2581.421 kb on + strand, within Psyr_2220 at 2581.422 kb on - strand, within Psyr_2220 at 2581.422 kb on - strand, within Psyr_2220 at 2581.422 kb on - strand, within Psyr_2220 at 2581.438 kb on - strand at 2581.559 kb on - strand at 2581.559 kb on - strand at 2581.562 kb on - strand at 2581.668 kb on + strand at 2581.709 kb on - strand at 2581.709 kb on - strand at 2581.715 kb on + strand at 2581.735 kb on + strand at 2581.736 kb on - strand at 2581.821 kb on + strand at 2581.835 kb on + strand at 2581.835 kb on + strand at 2581.835 kb on + strand at 2581.835 kb on + strand at 2581.835 kb on + strand at 2581.836 kb on - strand at 2581.836 kb on - strand at 2581.836 kb on - strand at 2581.836 kb on - strand at 2581.836 kb on - strand at 2581.836 kb on - strand at 2581.840 kb on - strand at 2581.887 kb on + strand at 2581.887 kb on + strand at 2581.887 kb on + strand at 2581.887 kb on + strand at 2581.888 kb on - strand at 2581.888 kb on - strand at 2582.109 kb on + strand, within Psyr_2221 at 2582.194 kb on - strand, within Psyr_2221 at 2582.354 kb on + strand at 2582.535 kb on + strand at 2582.828 kb on + strand, within Psyr_2222 at 2582.828 kb on + strand, within Psyr_2222 at 2582.828 kb on + strand, within Psyr_2222 at 2583.016 kb on - strand, within Psyr_2222 at 2583.174 kb on + strand, within Psyr_2222 at 2583.175 kb on - strand, within Psyr_2222 at 2583.210 kb on + strand, within Psyr_2222 at 2583.210 kb on + strand, within Psyr_2222 at 2583.211 kb on - strand, within Psyr_2222 at 2583.327 kb on + strand, within Psyr_2222 at 2583.327 kb on + strand, within Psyr_2222 at 2583.328 kb on - strand, within Psyr_2222 at 2583.417 kb on - strand, within Psyr_2222 at 2583.951 kb on - strand, within Psyr_2222 at 2583.951 kb on - strand, within Psyr_2222 at 2584.044 kb on + strand, within Psyr_2222 at 2584.080 kb on + strand, within Psyr_2222 at 2584.090 kb on + strand, within Psyr_2222 at 2584.091 kb on - strand, within Psyr_2222 at 2584.091 kb on - strand, within Psyr_2222 at 2584.092 kb on + strand, within Psyr_2222 at 2584.092 kb on + strand, within Psyr_2222 at 2584.093 kb on - strand, within Psyr_2222 at 2584.221 kb on + strand, within Psyr_2222 at 2584.221 kb on + strand, within Psyr_2222 at 2584.360 kb on - strand, within Psyr_2222 at 2584.413 kb on + strand, within Psyr_2222 at 2584.414 kb on - strand, within Psyr_2222 at 2584.537 kb on - strand, within Psyr_2222 at 2584.567 kb on - strand, within Psyr_2222 at 2584.567 kb on - strand, within Psyr_2222 at 2584.567 kb on - strand, within Psyr_2222 at 2584.567 kb on - strand, within Psyr_2222 at 2584.696 kb on - strand, within Psyr_2222 at 2584.696 kb on - strand, within Psyr_2222 at 2584.824 kb on + strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.825 kb on - strand, within Psyr_2222 at 2584.840 kb on - strand, within Psyr_2222 at 2584.929 kb on + strand, within Psyr_2222 at 2584.929 kb on + strand, within Psyr_2222 at 2584.930 kb on - strand, within Psyr_2222 at 2584.945 kb on - strand, within Psyr_2222 at 2585.069 kb on + strand at 2585.070 kb on - strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.148 kb on + strand at 2585.226 kb on + strand at 2585.226 kb on + strand at 2585.498 kb on + strand, within Psyr_2223 at 2585.499 kb on - strand, within Psyr_2223 at 2585.808 kb on + strand, within Psyr_2224 at 2585.808 kb on + strand, within Psyr_2224 at 2585.811 kb on + strand, within Psyr_2224 at 2585.812 kb on - strand at 2585.812 kb on - strand, within Psyr_2224 at 2585.847 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.036 kb on + strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.037 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224 at 2586.252 kb on - strand, within Psyr_2224
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 2,581,421 + Psyr_2220 0.90 +1.2 2,581,421 + Psyr_2220 0.90 +0.5 2,581,421 + Psyr_2220 0.90 -0.9 2,581,422 - Psyr_2220 0.90 -0.9 2,581,422 - Psyr_2220 0.90 +0.9 2,581,422 - Psyr_2220 0.90 -1.2 2,581,438 - -0.1 2,581,559 - +3.3 2,581,559 - +0.5 2,581,562 - +1.9 2,581,668 + +0.5 2,581,709 - -1.1 2,581,709 - -0.7 2,581,715 + -2.5 2,581,735 + +0.1 2,581,736 - -0.9 2,581,821 + -1.2 2,581,835 + -0.8 2,581,835 + +1.2 2,581,835 + -0.6 2,581,835 + +0.4 2,581,835 + -1.9 2,581,836 - -0.5 2,581,836 - -0.1 2,581,836 - +1.6 2,581,836 - +1.1 2,581,836 - -0.4 2,581,836 - -1.0 2,581,840 - -0.9 2,581,887 + +0.8 2,581,887 + -0.5 2,581,887 + -1.9 2,581,887 + -0.4 2,581,888 - +0.9 2,581,888 - -0.6 2,582,109 + Psyr_2221 0.48 +2.5 2,582,194 - Psyr_2221 0.63 -0.5 2,582,354 + -1.6 2,582,535 + -2.1 2,582,828 + Psyr_2222 0.14 -1.9 2,582,828 + Psyr_2222 0.14 +1.9 2,582,828 + Psyr_2222 0.14 +0.4 2,583,016 - Psyr_2222 0.21 +0.1 2,583,174 + Psyr_2222 0.26 -0.4 2,583,175 - Psyr_2222 0.26 -1.1 2,583,210 + Psyr_2222 0.28 -1.1 2,583,210 + Psyr_2222 0.28 +0.2 2,583,211 - Psyr_2222 0.28 +0.6 2,583,327 + Psyr_2222 0.32 +0.2 2,583,327 + Psyr_2222 0.32 -1.2 2,583,328 - Psyr_2222 0.32 -2.7 2,583,417 - Psyr_2222 0.35 +0.9 2,583,951 - Psyr_2222 0.53 -0.2 2,583,951 - Psyr_2222 0.53 -0.1 2,584,044 + Psyr_2222 0.56 -0.4 2,584,080 + Psyr_2222 0.58 +0.4 2,584,090 + Psyr_2222 0.58 +1.1 2,584,091 - Psyr_2222 0.58 -0.8 2,584,091 - Psyr_2222 0.58 -0.6 2,584,092 + Psyr_2222 0.58 -0.4 2,584,092 + Psyr_2222 0.58 -1.2 2,584,093 - Psyr_2222 0.58 +0.2 2,584,221 + Psyr_2222 0.62 -0.7 2,584,221 + Psyr_2222 0.62 +0.1 2,584,360 - Psyr_2222 0.67 -0.3 2,584,413 + Psyr_2222 0.69 -1.9 2,584,414 - Psyr_2222 0.69 -0.3 2,584,537 - Psyr_2222 0.73 -0.8 2,584,567 - Psyr_2222 0.74 -1.1 2,584,567 - Psyr_2222 0.74 -1.1 2,584,567 - Psyr_2222 0.74 -0.6 2,584,567 - Psyr_2222 0.74 -0.3 2,584,696 - Psyr_2222 0.79 -0.6 2,584,696 - Psyr_2222 0.79 -0.8 2,584,824 + Psyr_2222 0.83 -1.0 2,584,825 - Psyr_2222 0.83 -0.2 2,584,825 - Psyr_2222 0.83 +0.0 2,584,825 - Psyr_2222 0.83 -0.3 2,584,825 - Psyr_2222 0.83 -0.5 2,584,840 - Psyr_2222 0.84 +0.3 2,584,929 + Psyr_2222 0.87 -0.2 2,584,929 + Psyr_2222 0.87 +0.5 2,584,930 - Psyr_2222 0.87 -1.3 2,584,945 - Psyr_2222 0.87 -1.0 2,585,069 + -0.7 2,585,070 - -1.7 2,585,148 + -1.1 2,585,148 + -0.2 2,585,148 + -1.6 2,585,148 + -0.8 2,585,226 + +0.2 2,585,226 + -1.4 2,585,498 + Psyr_2223 0.61 +0.1 2,585,499 - Psyr_2223 0.61 -0.9 2,585,808 + Psyr_2224 0.14 -0.7 2,585,808 + Psyr_2224 0.14 +0.5 2,585,811 + Psyr_2224 0.15 -0.2 2,585,812 - +0.4 2,585,812 - Psyr_2224 0.15 -0.3 2,585,847 + Psyr_2224 0.17 +1.5 2,586,036 + Psyr_2224 0.33 -0.6 2,586,036 + Psyr_2224 0.33 +0.6 2,586,036 + Psyr_2224 0.33 +0.1 2,586,037 - Psyr_2224 0.33 -0.0 2,586,037 - Psyr_2224 0.33 +3.1 2,586,037 - Psyr_2224 0.33 -0.9 2,586,037 - Psyr_2224 0.33 +1.4 2,586,252 - Psyr_2224 0.50 -0.6 2,586,252 - Psyr_2224 0.50 +0.6 2,586,252 - Psyr_2224 0.50 -0.5 2,586,252 - Psyr_2224 0.50 -0.4 2,586,252 - Psyr_2224 0.50 -0.2
Or see this region's nucleotide sequence