Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2097 and Psyr_2098 are separated by 87 nucleotides Psyr_2098 and Psyr_2099 are separated by 10 nucleotides Psyr_2099 and Psyr_2100 overlap by 4 nucleotides
Psyr_2097: Psyr_2097 - Thrombospondin type 3 repeat:OmpA/MotB:OmpF, at 2,444,715 to 2,445,749
_2097
Psyr_2098: Psyr_2098 - uroporphyrinogen-III C-methyltransferase, at 2,445,837 to 2,446,598
_2098
Psyr_2099: Psyr_2099 - assimilatory nitrate reductase (NADH) alpha subunit apoprotein, at 2,446,609 to 2,449,329
_2099
Psyr_2100: Psyr_2100 - assimilatory nitrate reductase (NADH) beta subunit, at 2,449,326 to 2,450,555
_2100
Position (kb)
2446
2447
2448
2449
2450 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2445.618 kb on + strand, within Psyr_2097 at 2445.747 kb on + strand at 2445.748 kb on - strand at 2445.748 kb on - strand at 2445.895 kb on + strand at 2445.896 kb on - strand at 2446.099 kb on + strand, within Psyr_2098 at 2446.099 kb on + strand, within Psyr_2098 at 2446.099 kb on + strand, within Psyr_2098 at 2446.100 kb on - strand, within Psyr_2098 at 2446.100 kb on - strand, within Psyr_2098 at 2446.194 kb on + strand, within Psyr_2098 at 2446.194 kb on + strand, within Psyr_2098 at 2446.194 kb on + strand, within Psyr_2098 at 2446.195 kb on - strand, within Psyr_2098 at 2446.195 kb on - strand, within Psyr_2098 at 2446.197 kb on - strand, within Psyr_2098 at 2446.251 kb on - strand, within Psyr_2098 at 2446.251 kb on - strand, within Psyr_2098 at 2446.376 kb on - strand, within Psyr_2098 at 2446.584 kb on + strand at 2446.584 kb on + strand at 2446.584 kb on + strand at 2446.584 kb on + strand at 2446.585 kb on - strand at 2446.585 kb on - strand at 2446.585 kb on - strand at 2446.645 kb on + strand at 2446.646 kb on - strand at 2446.646 kb on - strand at 2446.926 kb on + strand, within Psyr_2099 at 2446.926 kb on + strand, within Psyr_2099 at 2446.927 kb on - strand, within Psyr_2099 at 2446.927 kb on - strand, within Psyr_2099 at 2446.979 kb on + strand, within Psyr_2099 at 2447.011 kb on + strand, within Psyr_2099 at 2447.072 kb on - strand, within Psyr_2099 at 2447.114 kb on + strand, within Psyr_2099 at 2447.114 kb on + strand, within Psyr_2099 at 2447.115 kb on - strand, within Psyr_2099 at 2447.591 kb on + strand, within Psyr_2099 at 2447.591 kb on + strand, within Psyr_2099 at 2447.591 kb on + strand, within Psyr_2099 at 2447.591 kb on + strand, within Psyr_2099 at 2447.592 kb on - strand, within Psyr_2099 at 2447.592 kb on - strand, within Psyr_2099 at 2447.592 kb on - strand, within Psyr_2099 at 2447.638 kb on - strand, within Psyr_2099 at 2448.406 kb on - strand, within Psyr_2099 at 2448.492 kb on + strand, within Psyr_2099 at 2448.558 kb on - strand, within Psyr_2099 at 2448.613 kb on + strand, within Psyr_2099 at 2448.613 kb on + strand, within Psyr_2099 at 2448.613 kb on + strand, within Psyr_2099 at 2448.613 kb on + strand, within Psyr_2099 at 2448.613 kb on + strand, within Psyr_2099 at 2448.613 kb on + strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.614 kb on - strand, within Psyr_2099 at 2448.615 kb on + strand, within Psyr_2099 at 2448.615 kb on + strand, within Psyr_2099 at 2448.615 kb on + strand, within Psyr_2099 at 2448.615 kb on + strand, within Psyr_2099 at 2448.615 kb on + strand, within Psyr_2099 at 2448.616 kb on - strand, within Psyr_2099 at 2448.616 kb on - strand, within Psyr_2099 at 2448.616 kb on - strand at 2448.616 kb on - strand, within Psyr_2099 at 2448.616 kb on - strand, within Psyr_2099 at 2448.616 kb on - strand, within Psyr_2099 at 2448.783 kb on - strand, within Psyr_2099 at 2448.829 kb on - strand, within Psyr_2099 at 2448.875 kb on + strand, within Psyr_2099 at 2448.965 kb on + strand, within Psyr_2099 at 2448.966 kb on - strand, within Psyr_2099 at 2448.966 kb on - strand, within Psyr_2099 at 2448.968 kb on + strand, within Psyr_2099 at 2448.968 kb on + strand, within Psyr_2099 at 2448.968 kb on + strand, within Psyr_2099 at 2448.968 kb on + strand, within Psyr_2099 at 2448.968 kb on + strand, within Psyr_2099 at 2448.968 kb on + strand, within Psyr_2099 at 2448.999 kb on - strand, within Psyr_2099 at 2449.125 kb on - strand at 2449.230 kb on + strand at 2449.230 kb on + strand at 2449.277 kb on + strand at 2449.278 kb on - strand at 2449.420 kb on + strand at 2449.421 kb on - strand at 2449.468 kb on - strand, within Psyr_2100 at 2449.496 kb on - strand, within Psyr_2100 at 2449.589 kb on + strand, within Psyr_2100 at 2449.590 kb on - strand, within Psyr_2100 at 2449.732 kb on + strand, within Psyr_2100 at 2449.733 kb on - strand, within Psyr_2100 at 2449.747 kb on + strand, within Psyr_2100 at 2449.748 kb on - strand, within Psyr_2100 at 2449.827 kb on + strand, within Psyr_2100 at 2449.827 kb on + strand, within Psyr_2100 at 2450.031 kb on - strand, within Psyr_2100 at 2450.036 kb on + strand, within Psyr_2100 at 2450.251 kb on + strand, within Psyr_2100 at 2450.251 kb on + strand, within Psyr_2100
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 2,445,618 + Psyr_2097 0.87 -2.3 2,445,747 + +1.2 2,445,748 - -1.7 2,445,748 - -1.2 2,445,895 + +1.5 2,445,896 - +0.1 2,446,099 + Psyr_2098 0.34 -0.7 2,446,099 + Psyr_2098 0.34 +0.7 2,446,099 + Psyr_2098 0.34 +0.4 2,446,100 - Psyr_2098 0.35 -0.7 2,446,100 - Psyr_2098 0.35 +0.8 2,446,194 + Psyr_2098 0.47 -0.7 2,446,194 + Psyr_2098 0.47 -0.9 2,446,194 + Psyr_2098 0.47 +0.8 2,446,195 - Psyr_2098 0.47 -0.7 2,446,195 - Psyr_2098 0.47 -0.3 2,446,197 - Psyr_2098 0.47 -0.7 2,446,251 - Psyr_2098 0.54 -0.2 2,446,251 - Psyr_2098 0.54 -0.4 2,446,376 - Psyr_2098 0.71 -0.9 2,446,584 + +0.1 2,446,584 + -0.9 2,446,584 + +1.5 2,446,584 + -0.0 2,446,585 - -2.2 2,446,585 - -0.2 2,446,585 - -3.4 2,446,645 + -0.2 2,446,646 - -1.2 2,446,646 - +1.4 2,446,926 + Psyr_2099 0.12 -0.2 2,446,926 + Psyr_2099 0.12 +0.3 2,446,927 - Psyr_2099 0.12 -0.4 2,446,927 - Psyr_2099 0.12 -0.6 2,446,979 + Psyr_2099 0.14 +0.3 2,447,011 + Psyr_2099 0.15 +0.7 2,447,072 - Psyr_2099 0.17 -1.9 2,447,114 + Psyr_2099 0.19 -0.9 2,447,114 + Psyr_2099 0.19 -0.7 2,447,115 - Psyr_2099 0.19 -0.2 2,447,591 + Psyr_2099 0.36 +0.3 2,447,591 + Psyr_2099 0.36 +1.6 2,447,591 + Psyr_2099 0.36 +1.3 2,447,591 + Psyr_2099 0.36 +0.1 2,447,592 - Psyr_2099 0.36 -0.2 2,447,592 - Psyr_2099 0.36 -0.1 2,447,592 - Psyr_2099 0.36 +0.3 2,447,638 - Psyr_2099 0.38 +0.2 2,448,406 - Psyr_2099 0.66 +1.1 2,448,492 + Psyr_2099 0.69 -0.6 2,448,558 - Psyr_2099 0.72 +0.8 2,448,613 + Psyr_2099 0.74 +0.8 2,448,613 + Psyr_2099 0.74 -1.3 2,448,613 + Psyr_2099 0.74 +2.8 2,448,613 + Psyr_2099 0.74 +0.5 2,448,613 + Psyr_2099 0.74 +1.2 2,448,613 + Psyr_2099 0.74 -0.2 2,448,614 - Psyr_2099 0.74 -0.6 2,448,614 - Psyr_2099 0.74 -0.4 2,448,614 - Psyr_2099 0.74 -0.2 2,448,614 - Psyr_2099 0.74 +1.8 2,448,614 - Psyr_2099 0.74 +0.1 2,448,614 - Psyr_2099 0.74 +1.3 2,448,614 - Psyr_2099 0.74 +0.5 2,448,614 - Psyr_2099 0.74 +0.8 2,448,614 - Psyr_2099 0.74 +0.8 2,448,614 - Psyr_2099 0.74 +0.3 2,448,614 - Psyr_2099 0.74 +0.7 2,448,614 - Psyr_2099 0.74 -0.8 2,448,615 + Psyr_2099 0.74 -0.3 2,448,615 + Psyr_2099 0.74 +0.0 2,448,615 + Psyr_2099 0.74 -0.4 2,448,615 + Psyr_2099 0.74 -0.5 2,448,615 + Psyr_2099 0.74 -0.2 2,448,616 - Psyr_2099 0.74 +0.4 2,448,616 - Psyr_2099 0.74 -1.0 2,448,616 - +1.8 2,448,616 - Psyr_2099 0.74 +0.8 2,448,616 - Psyr_2099 0.74 +0.8 2,448,616 - Psyr_2099 0.74 +4.2 2,448,783 - Psyr_2099 0.80 +2.2 2,448,829 - Psyr_2099 0.82 +0.2 2,448,875 + Psyr_2099 0.83 +0.2 2,448,965 + Psyr_2099 0.87 -0.2 2,448,966 - Psyr_2099 0.87 +1.4 2,448,966 - Psyr_2099 0.87 +0.2 2,448,968 + Psyr_2099 0.87 +0.4 2,448,968 + Psyr_2099 0.87 -1.3 2,448,968 + Psyr_2099 0.87 +2.4 2,448,968 + Psyr_2099 0.87 -1.1 2,448,968 + Psyr_2099 0.87 -3.0 2,448,968 + Psyr_2099 0.87 -0.0 2,448,999 - Psyr_2099 0.88 +0.3 2,449,125 - -1.2 2,449,230 + -0.7 2,449,230 + -0.1 2,449,277 + -0.3 2,449,278 - -0.1 2,449,420 + -0.5 2,449,421 - +0.7 2,449,468 - Psyr_2100 0.12 -0.1 2,449,496 - Psyr_2100 0.14 +2.0 2,449,589 + Psyr_2100 0.21 +0.4 2,449,590 - Psyr_2100 0.21 -0.4 2,449,732 + Psyr_2100 0.33 +0.2 2,449,733 - Psyr_2100 0.33 +0.0 2,449,747 + Psyr_2100 0.34 +0.6 2,449,748 - Psyr_2100 0.34 +0.6 2,449,827 + Psyr_2100 0.41 -0.2 2,449,827 + Psyr_2100 0.41 +2.4 2,450,031 - Psyr_2100 0.57 +0.7 2,450,036 + Psyr_2100 0.58 -0.2 2,450,251 + Psyr_2100 0.75 +0.2 2,450,251 + Psyr_2100 0.75 -1.3
Or see this region's nucleotide sequence