Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1599 and Psyr_1600 are separated by 105 nucleotides Psyr_1600 and Psyr_1601 are separated by 19 nucleotides Psyr_1601 and Psyr_1602 are separated by 84 nucleotides
Psyr_1599: Psyr_1599 - C-terminal processing peptidase-1, Serine peptidase, MEROPS family S41A, at 1,797,634 to 1,799,748
_1599
Psyr_1600: Psyr_1600 - Zinc-containing alcohol dehydrogenase superfamily, at 1,799,854 to 1,800,816
_1600
Psyr_1601: Psyr_1601 - HAD-superfamily hydrolase, subfamily IB (PSPase-like):HAD-superfamily subfamily IB hydrolase, hypothetical 2, at 1,800,836 to 1,801,489
_1601
Psyr_1602: Psyr_1602 - ABC transporter, at 1,801,574 to 1,802,563
_1602
Position (kb)
1799
1800
1801 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1799.027 kb on + strand, within Psyr_1599 at 1799.027 kb on + strand, within Psyr_1599 at 1799.027 kb on + strand, within Psyr_1599 at 1799.027 kb on + strand, within Psyr_1599 at 1799.028 kb on - strand, within Psyr_1599 at 1799.028 kb on - strand, within Psyr_1599 at 1799.028 kb on - strand, within Psyr_1599 at 1799.028 kb on - strand, within Psyr_1599 at 1799.069 kb on + strand, within Psyr_1599 at 1799.069 kb on + strand, within Psyr_1599 at 1799.070 kb on + strand, within Psyr_1599 at 1799.070 kb on - strand, within Psyr_1599 at 1799.070 kb on - strand, within Psyr_1599 at 1799.070 kb on - strand, within Psyr_1599 at 1799.070 kb on - strand, within Psyr_1599 at 1799.082 kb on + strand, within Psyr_1599 at 1799.082 kb on + strand, within Psyr_1599 at 1799.083 kb on - strand, within Psyr_1599 at 1799.083 kb on - strand, within Psyr_1599 at 1799.084 kb on - strand, within Psyr_1599 at 1799.124 kb on + strand, within Psyr_1599 at 1799.124 kb on + strand, within Psyr_1599 at 1799.125 kb on - strand, within Psyr_1599 at 1799.125 kb on - strand, within Psyr_1599 at 1799.126 kb on + strand, within Psyr_1599 at 1799.286 kb on + strand, within Psyr_1599 at 1799.372 kb on + strand, within Psyr_1599 at 1799.372 kb on + strand, within Psyr_1599 at 1799.372 kb on + strand, within Psyr_1599 at 1799.372 kb on + strand, within Psyr_1599 at 1799.373 kb on - strand, within Psyr_1599 at 1799.373 kb on - strand, within Psyr_1599 at 1799.373 kb on - strand, within Psyr_1599 at 1799.468 kb on + strand, within Psyr_1599 at 1799.468 kb on + strand, within Psyr_1599 at 1799.468 kb on + strand, within Psyr_1599 at 1799.468 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.498 kb on + strand, within Psyr_1599 at 1799.499 kb on - strand, within Psyr_1599 at 1799.499 kb on - strand, within Psyr_1599 at 1799.499 kb on - strand, within Psyr_1599 at 1799.499 kb on - strand, within Psyr_1599 at 1799.499 kb on - strand, within Psyr_1599 at 1799.499 kb on - strand, within Psyr_1599 at 1799.499 kb on - strand, within Psyr_1599 at 1799.499 kb on - strand, within Psyr_1599 at 1799.507 kb on + strand, within Psyr_1599 at 1799.507 kb on + strand, within Psyr_1599 at 1799.507 kb on + strand, within Psyr_1599 at 1799.508 kb on - strand, within Psyr_1599 at 1799.512 kb on - strand, within Psyr_1599 at 1799.549 kb on + strand at 1799.549 kb on + strand at 1799.549 kb on + strand at 1799.550 kb on - strand at 1799.643 kb on + strand at 1799.644 kb on - strand at 1799.704 kb on + strand at 1799.705 kb on - strand at 1799.705 kb on - strand at 1799.748 kb on + strand at 1799.749 kb on - strand at 1799.749 kb on - strand at 1799.749 kb on - strand at 1799.752 kb on - strand at 1799.842 kb on + strand at 1799.842 kb on + strand at 1799.930 kb on + strand at 1799.930 kb on + strand at 1799.930 kb on + strand at 1799.931 kb on - strand at 1799.931 kb on - strand at 1799.934 kb on + strand at 1799.934 kb on + strand at 1799.935 kb on - strand at 1799.935 kb on - strand at 1799.935 kb on - strand at 1799.935 kb on - strand at 1799.976 kb on - strand, within Psyr_1600 at 1799.992 kb on + strand, within Psyr_1600 at 1799.992 kb on + strand, within Psyr_1600 at 1800.208 kb on + strand, within Psyr_1600 at 1800.208 kb on + strand, within Psyr_1600 at 1800.208 kb on + strand, within Psyr_1600 at 1800.373 kb on + strand, within Psyr_1600 at 1800.374 kb on - strand, within Psyr_1600 at 1800.481 kb on + strand, within Psyr_1600 at 1800.497 kb on + strand, within Psyr_1600 at 1800.498 kb on - strand, within Psyr_1600 at 1800.498 kb on - strand, within Psyr_1600 at 1800.768 kb on + strand at 1800.885 kb on - strand at 1801.221 kb on + strand, within Psyr_1601 at 1801.222 kb on - strand, within Psyr_1601 at 1801.222 kb on - strand, within Psyr_1601 at 1801.222 kb on - strand, within Psyr_1601 at 1801.222 kb on - strand, within Psyr_1601 at 1801.222 kb on - strand, within Psyr_1601 at 1801.222 kb on - strand, within Psyr_1601 at 1801.222 kb on - strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.320 kb on + strand, within Psyr_1601 at 1801.321 kb on - strand, within Psyr_1601 at 1801.321 kb on - strand, within Psyr_1601 at 1801.350 kb on + strand, within Psyr_1601 at 1801.351 kb on - strand, within Psyr_1601 at 1801.498 kb on - strand at 1801.514 kb on - strand at 1801.518 kb on + strand at 1801.616 kb on + strand at 1801.616 kb on + strand at 1801.616 kb on + strand at 1801.617 kb on - strand at 1801.661 kb on + strand at 1801.662 kb on - strand at 1801.662 kb on - strand at 1801.736 kb on + strand, within Psyr_1602 at 1801.737 kb on - strand, within Psyr_1602
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 1,799,027 + Psyr_1599 0.66 +1.7 1,799,027 + Psyr_1599 0.66 +0.1 1,799,027 + Psyr_1599 0.66 -0.1 1,799,027 + Psyr_1599 0.66 +0.2 1,799,028 - Psyr_1599 0.66 +0.1 1,799,028 - Psyr_1599 0.66 +0.6 1,799,028 - Psyr_1599 0.66 +0.3 1,799,028 - Psyr_1599 0.66 -0.5 1,799,069 + Psyr_1599 0.68 -0.8 1,799,069 + Psyr_1599 0.68 -0.7 1,799,070 + Psyr_1599 0.68 -0.9 1,799,070 - Psyr_1599 0.68 -1.9 1,799,070 - Psyr_1599 0.68 +0.7 1,799,070 - Psyr_1599 0.68 +0.3 1,799,070 - Psyr_1599 0.68 +0.2 1,799,082 + Psyr_1599 0.68 -0.3 1,799,082 + Psyr_1599 0.68 -0.9 1,799,083 - Psyr_1599 0.69 -0.2 1,799,083 - Psyr_1599 0.69 +0.6 1,799,084 - Psyr_1599 0.69 -0.3 1,799,124 + Psyr_1599 0.70 +2.1 1,799,124 + Psyr_1599 0.70 -1.4 1,799,125 - Psyr_1599 0.70 -1.0 1,799,125 - Psyr_1599 0.70 -1.7 1,799,126 + Psyr_1599 0.71 -0.1 1,799,286 + Psyr_1599 0.78 -1.6 1,799,372 + Psyr_1599 0.82 +0.8 1,799,372 + Psyr_1599 0.82 +0.5 1,799,372 + Psyr_1599 0.82 +0.7 1,799,372 + Psyr_1599 0.82 +0.2 1,799,373 - Psyr_1599 0.82 -0.4 1,799,373 - Psyr_1599 0.82 -0.3 1,799,373 - Psyr_1599 0.82 +0.1 1,799,468 + Psyr_1599 0.87 -1.6 1,799,468 + Psyr_1599 0.87 +0.5 1,799,468 + Psyr_1599 0.87 -0.7 1,799,468 + Psyr_1599 0.87 +1.8 1,799,498 + Psyr_1599 0.88 -1.0 1,799,498 + Psyr_1599 0.88 +0.2 1,799,498 + Psyr_1599 0.88 -0.6 1,799,498 + Psyr_1599 0.88 +1.3 1,799,498 + Psyr_1599 0.88 -0.5 1,799,498 + Psyr_1599 0.88 -0.4 1,799,498 + Psyr_1599 0.88 -0.6 1,799,498 + Psyr_1599 0.88 +2.0 1,799,498 + Psyr_1599 0.88 +0.7 1,799,499 - Psyr_1599 0.88 -0.1 1,799,499 - Psyr_1599 0.88 -1.4 1,799,499 - Psyr_1599 0.88 -3.0 1,799,499 - Psyr_1599 0.88 -0.8 1,799,499 - Psyr_1599 0.88 +0.1 1,799,499 - Psyr_1599 0.88 -0.7 1,799,499 - Psyr_1599 0.88 -0.8 1,799,499 - Psyr_1599 0.88 -0.3 1,799,507 + Psyr_1599 0.89 -0.1 1,799,507 + Psyr_1599 0.89 -0.1 1,799,507 + Psyr_1599 0.89 +0.7 1,799,508 - Psyr_1599 0.89 -0.6 1,799,512 - Psyr_1599 0.89 -0.1 1,799,549 + -1.2 1,799,549 + +0.9 1,799,549 + -0.3 1,799,550 - -0.6 1,799,643 + +0.2 1,799,644 - +0.2 1,799,704 + -0.9 1,799,705 - +0.1 1,799,705 - -1.9 1,799,748 + -0.5 1,799,749 - +0.3 1,799,749 - +0.5 1,799,749 - -3.1 1,799,752 - +0.2 1,799,842 + -0.6 1,799,842 + -3.4 1,799,930 + -1.5 1,799,930 + +1.1 1,799,930 + +0.2 1,799,931 - +3.9 1,799,931 - -1.6 1,799,934 + +0.8 1,799,934 + -0.4 1,799,935 - +0.2 1,799,935 - +0.2 1,799,935 - -0.6 1,799,935 - -0.9 1,799,976 - Psyr_1600 0.13 +1.9 1,799,992 + Psyr_1600 0.14 -0.5 1,799,992 + Psyr_1600 0.14 +0.8 1,800,208 + Psyr_1600 0.37 +3.5 1,800,208 + Psyr_1600 0.37 -0.9 1,800,208 + Psyr_1600 0.37 +2.0 1,800,373 + Psyr_1600 0.54 -0.1 1,800,374 - Psyr_1600 0.54 -0.6 1,800,481 + Psyr_1600 0.65 +0.1 1,800,497 + Psyr_1600 0.67 +0.5 1,800,498 - Psyr_1600 0.67 +1.9 1,800,498 - Psyr_1600 0.67 -0.1 1,800,768 + -1.5 1,800,885 - +0.4 1,801,221 + Psyr_1601 0.59 -1.5 1,801,222 - Psyr_1601 0.59 -0.3 1,801,222 - Psyr_1601 0.59 -0.8 1,801,222 - Psyr_1601 0.59 -0.6 1,801,222 - Psyr_1601 0.59 +0.3 1,801,222 - Psyr_1601 0.59 -0.7 1,801,222 - Psyr_1601 0.59 -0.9 1,801,222 - Psyr_1601 0.59 -1.1 1,801,320 + Psyr_1601 0.74 -1.2 1,801,320 + Psyr_1601 0.74 -0.9 1,801,320 + Psyr_1601 0.74 +1.4 1,801,320 + Psyr_1601 0.74 -0.1 1,801,320 + Psyr_1601 0.74 +0.7 1,801,320 + Psyr_1601 0.74 -1.9 1,801,320 + Psyr_1601 0.74 +0.4 1,801,320 + Psyr_1601 0.74 -0.1 1,801,320 + Psyr_1601 0.74 -1.3 1,801,320 + Psyr_1601 0.74 -0.9 1,801,320 + Psyr_1601 0.74 -1.8 1,801,320 + Psyr_1601 0.74 -1.9 1,801,320 + Psyr_1601 0.74 -1.3 1,801,320 + Psyr_1601 0.74 -0.2 1,801,320 + Psyr_1601 0.74 +0.1 1,801,320 + Psyr_1601 0.74 -2.1 1,801,321 - Psyr_1601 0.74 +0.7 1,801,321 - Psyr_1601 0.74 +0.2 1,801,350 + Psyr_1601 0.79 +0.0 1,801,351 - Psyr_1601 0.79 -2.7 1,801,498 - +0.5 1,801,514 - -1.2 1,801,518 + +0.7 1,801,616 + +0.0 1,801,616 + +0.7 1,801,616 + -1.6 1,801,617 - -0.6 1,801,661 + -2.1 1,801,662 - -0.4 1,801,662 - -0.1 1,801,736 + Psyr_1602 0.16 +0.8 1,801,737 - Psyr_1602 0.16 -1.1
Or see this region's nucleotide sequence