Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1406 and Psyr_1407 are separated by 117 nucleotides Psyr_1407 and Psyr_1408 are separated by 130 nucleotides Psyr_1408 and Psyr_1409 are separated by 85 nucleotides
Psyr_1406: Psyr_1406 - aspartate--tRNA(Asn) ligase, at 1,592,180 to 1,593,955
_1406
Psyr_1407: Psyr_1407 - Protein of unknown function DUF28, at 1,594,073 to 1,594,819
_1407
Psyr_1408: Psyr_1408 - Holliday junction endonuclease RuvC, at 1,594,950 to 1,595,474
_1408
Psyr_1409: Psyr_1409 - Holliday junction DNA helicase subunit RuvA, at 1,595,560 to 1,596,168
_1409
Position (kb)
1594
1595 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1593.993 kb on - strand at 1594.087 kb on + strand at 1594.087 kb on + strand at 1594.087 kb on + strand at 1594.614 kb on - strand, within Psyr_1407 at 1594.614 kb on - strand, within Psyr_1407 at 1594.722 kb on + strand, within Psyr_1407 at 1594.723 kb on - strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.738 kb on + strand, within Psyr_1407 at 1594.739 kb on - strand, within Psyr_1407 at 1594.739 kb on - strand, within Psyr_1407 at 1594.739 kb on - strand, within Psyr_1407 at 1594.772 kb on + strand at 1594.772 kb on + strand at 1594.772 kb on + strand at 1594.772 kb on + strand at 1594.773 kb on - strand at 1594.773 kb on - strand at 1594.831 kb on + strand at 1594.832 kb on - strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.914 kb on + strand at 1594.949 kb on + strand at 1594.950 kb on - strand at 1594.958 kb on + strand at 1594.958 kb on + strand at 1594.959 kb on - strand at 1594.959 kb on - strand at 1594.959 kb on - strand at 1594.959 kb on - strand at 1594.996 kb on - strand at 1595.031 kb on + strand, within Psyr_1408 at 1595.031 kb on + strand, within Psyr_1408 at 1595.032 kb on - strand, within Psyr_1408 at 1595.125 kb on - strand, within Psyr_1408 at 1595.253 kb on + strand, within Psyr_1408 at 1595.254 kb on - strand, within Psyr_1408 at 1595.406 kb on - strand, within Psyr_1408 at 1595.473 kb on - strand at 1595.473 kb on - strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.481 kb on + strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.482 kb on - strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.483 kb on + strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.484 kb on - strand at 1595.493 kb on + strand at 1595.494 kb on - strand at 1595.494 kb on - strand at 1595.494 kb on - strand at 1595.501 kb on - strand at 1595.573 kb on + strand at 1595.741 kb on - strand, within Psyr_1409
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 1,593,993 - -0.2 1,594,087 + -3.5 1,594,087 + -0.1 1,594,087 + +0.5 1,594,614 - Psyr_1407 0.72 -0.9 1,594,614 - Psyr_1407 0.72 -0.7 1,594,722 + Psyr_1407 0.87 -0.5 1,594,723 - Psyr_1407 0.87 -1.0 1,594,738 + Psyr_1407 0.89 -0.9 1,594,738 + Psyr_1407 0.89 -0.6 1,594,738 + Psyr_1407 0.89 -0.2 1,594,738 + Psyr_1407 0.89 -0.2 1,594,739 - Psyr_1407 0.89 -1.6 1,594,739 - Psyr_1407 0.89 -1.5 1,594,739 - Psyr_1407 0.89 -0.9 1,594,772 + -0.3 1,594,772 + -0.2 1,594,772 + +1.0 1,594,772 + +0.4 1,594,773 - -0.2 1,594,773 - -0.4 1,594,831 + -0.7 1,594,832 - -1.0 1,594,914 + -0.9 1,594,914 + -0.0 1,594,914 + -0.6 1,594,949 + +2.0 1,594,950 - -0.5 1,594,958 + +0.5 1,594,958 + -1.0 1,594,959 - -1.9 1,594,959 - -1.1 1,594,959 - -1.7 1,594,959 - +0.5 1,594,996 - +1.2 1,595,031 + Psyr_1408 0.15 -0.0 1,595,031 + Psyr_1408 0.15 +0.4 1,595,032 - Psyr_1408 0.16 -1.9 1,595,125 - Psyr_1408 0.33 -1.2 1,595,253 + Psyr_1408 0.58 +2.2 1,595,254 - Psyr_1408 0.58 -2.1 1,595,406 - Psyr_1408 0.87 -1.2 1,595,473 - -1.4 1,595,473 - -1.6 1,595,481 + +0.0 1,595,481 + +0.2 1,595,481 + -0.3 1,595,481 + +1.3 1,595,481 + +0.5 1,595,481 + +0.5 1,595,481 + -0.9 1,595,481 + -0.6 1,595,481 + +1.0 1,595,481 + +1.5 1,595,481 + +0.2 1,595,481 + +0.7 1,595,482 - -1.7 1,595,482 - -0.0 1,595,482 - -1.0 1,595,482 - -2.5 1,595,482 - +1.5 1,595,482 - -0.5 1,595,482 - -1.5 1,595,482 - -0.0 1,595,482 - -1.9 1,595,483 + +3.3 1,595,483 + -0.2 1,595,483 + -0.3 1,595,483 + +0.8 1,595,483 + -0.9 1,595,483 + -0.1 1,595,483 + -0.8 1,595,483 + -0.0 1,595,483 + -0.2 1,595,483 + +1.0 1,595,483 + +0.5 1,595,483 + +0.3 1,595,483 + -0.9 1,595,483 + +0.7 1,595,483 + +1.8 1,595,483 + -0.6 1,595,483 + -0.6 1,595,483 + -0.3 1,595,483 + +0.4 1,595,483 + +1.2 1,595,483 + +0.7 1,595,483 + -0.7 1,595,483 + -1.7 1,595,483 + -0.6 1,595,483 + -2.2 1,595,483 + -0.5 1,595,483 + +0.5 1,595,483 + +1.0 1,595,484 - -0.4 1,595,484 - -0.5 1,595,484 - -2.0 1,595,484 - -3.4 1,595,484 - -1.2 1,595,484 - -0.6 1,595,484 - -0.2 1,595,484 - -1.0 1,595,484 - -0.1 1,595,484 - -0.2 1,595,484 - -2.2 1,595,484 - +0.1 1,595,484 - +0.1 1,595,484 - -1.4 1,595,484 - -0.6 1,595,484 - +2.8 1,595,493 + +0.2 1,595,494 - +0.6 1,595,494 - +2.5 1,595,494 - -0.2 1,595,501 - +0.2 1,595,573 + +1.0 1,595,741 - Psyr_1409 0.30 -1.6
Or see this region's nucleotide sequence