Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1200 and Psyr_1201 overlap by 4 nucleotides Psyr_1201 and Psyr_1202 are separated by 2 nucleotides Psyr_1202 and Psyr_1203 are separated by 602 nucleotides
Psyr_1200: Psyr_1200 - outer-membrane type III secretion protein HrcC, at 1,357,786 to 1,359,888
_1200
Psyr_1201: Psyr_1201 - type III secretion protein HrpT, at 1,359,885 to 1,360,088
_1201
Psyr_1202: Psyr_1202 - negative regulator of hrp expression HrpV, at 1,360,091 to 1,360,438
_1202
Psyr_1203: Psyr_1203 - hypothetical protein, at 1,361,041 to 1,361,901
_1203
Position (kb)
1360
1361 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1359.221 kb on + strand, within Psyr_1200 at 1359.313 kb on + strand, within Psyr_1200 at 1359.314 kb on - strand, within Psyr_1200 at 1359.314 kb on - strand, within Psyr_1200 at 1359.421 kb on + strand, within Psyr_1200 at 1359.421 kb on + strand, within Psyr_1200 at 1359.421 kb on + strand, within Psyr_1200 at 1359.421 kb on + strand, within Psyr_1200 at 1359.422 kb on - strand, within Psyr_1200 at 1359.652 kb on + strand, within Psyr_1200 at 1359.652 kb on + strand, within Psyr_1200 at 1359.653 kb on - strand, within Psyr_1200 at 1359.653 kb on - strand, within Psyr_1200 at 1359.653 kb on - strand, within Psyr_1200 at 1359.653 kb on - strand, within Psyr_1200 at 1359.653 kb on - strand, within Psyr_1200 at 1359.653 kb on - strand, within Psyr_1200 at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.808 kb on + strand at 1359.809 kb on - strand at 1359.809 kb on - strand at 1359.809 kb on - strand at 1359.809 kb on - strand at 1359.809 kb on - strand at 1359.809 kb on - strand at 1359.818 kb on + strand at 1359.819 kb on - strand at 1359.819 kb on - strand at 1360.062 kb on + strand, within Psyr_1201 at 1360.078 kb on + strand at 1360.086 kb on + strand at 1360.086 kb on + strand at 1360.087 kb on - strand at 1360.121 kb on + strand at 1360.122 kb on - strand at 1360.122 kb on - strand at 1360.122 kb on - strand at 1360.124 kb on + strand at 1360.124 kb on + strand at 1360.124 kb on + strand at 1360.124 kb on + strand at 1360.125 kb on - strand at 1360.125 kb on - strand at 1360.304 kb on + strand, within Psyr_1202 at 1360.305 kb on - strand, within Psyr_1202 at 1360.305 kb on - strand, within Psyr_1202 at 1360.411 kb on - strand at 1360.444 kb on - strand at 1360.535 kb on + strand at 1360.564 kb on + strand at 1360.565 kb on - strand at 1360.574 kb on + strand at 1360.574 kb on + strand at 1360.575 kb on - strand at 1360.578 kb on - strand at 1360.625 kb on - strand at 1360.625 kb on - strand at 1360.625 kb on - strand at 1360.626 kb on + strand at 1360.626 kb on + strand at 1360.626 kb on + strand at 1360.627 kb on - strand at 1360.627 kb on - strand at 1360.627 kb on - strand at 1360.630 kb on + strand at 1360.630 kb on + strand at 1360.630 kb on + strand at 1360.631 kb on - strand at 1360.631 kb on - strand at 1360.631 kb on - strand at 1360.631 kb on - strand at 1360.631 kb on - strand at 1360.631 kb on - strand at 1360.700 kb on + strand at 1360.700 kb on + strand at 1360.701 kb on - strand at 1360.701 kb on - strand at 1360.709 kb on + strand at 1360.710 kb on - strand at 1360.710 kb on - strand at 1360.710 kb on - strand at 1360.710 kb on - strand at 1360.790 kb on + strand at 1360.791 kb on - strand at 1360.800 kb on - strand at 1360.836 kb on + strand at 1360.836 kb on + strand at 1360.837 kb on - strand at 1360.837 kb on - strand at 1360.881 kb on + strand at 1360.882 kb on - strand at 1360.922 kb on + strand at 1360.926 kb on - strand at 1360.939 kb on + strand at 1360.939 kb on + strand at 1360.939 kb on + strand at 1360.939 kb on + strand at 1360.939 kb on + strand at 1360.939 kb on + strand at 1360.940 kb on - strand at 1360.940 kb on - strand at 1360.940 kb on - strand at 1360.940 kb on - strand at 1360.940 kb on - strand at 1360.948 kb on + strand at 1360.949 kb on - strand at 1360.992 kb on + strand at 1360.993 kb on - strand at 1360.998 kb on - strand at 1360.998 kb on - strand at 1361.042 kb on + strand at 1361.043 kb on - strand at 1361.043 kb on - strand at 1361.047 kb on - strand at 1361.138 kb on + strand, within Psyr_1203 at 1361.138 kb on + strand, within Psyr_1203 at 1361.139 kb on - strand, within Psyr_1203 at 1361.139 kb on - strand, within Psyr_1203 at 1361.139 kb on - strand, within Psyr_1203 at 1361.157 kb on + strand, within Psyr_1203 at 1361.158 kb on - strand, within Psyr_1203 at 1361.158 kb on - strand, within Psyr_1203 at 1361.160 kb on + strand, within Psyr_1203 at 1361.270 kb on + strand, within Psyr_1203 at 1361.270 kb on + strand, within Psyr_1203 at 1361.317 kb on + strand, within Psyr_1203 at 1361.317 kb on + strand, within Psyr_1203 at 1361.318 kb on - strand, within Psyr_1203 at 1361.341 kb on - strand, within Psyr_1203 at 1361.355 kb on + strand, within Psyr_1203 at 1361.355 kb on + strand, within Psyr_1203 at 1361.409 kb on + strand, within Psyr_1203 at 1361.438 kb on + strand, within Psyr_1203 at 1361.438 kb on + strand, within Psyr_1203 at 1361.438 kb on + strand, within Psyr_1203 at 1361.438 kb on + strand, within Psyr_1203 at 1361.438 kb on + strand, within Psyr_1203
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 1,359,221 + Psyr_1200 0.68 +0.9 1,359,313 + Psyr_1200 0.73 +0.4 1,359,314 - Psyr_1200 0.73 -0.8 1,359,314 - Psyr_1200 0.73 +0.5 1,359,421 + Psyr_1200 0.78 +1.4 1,359,421 + Psyr_1200 0.78 +1.8 1,359,421 + Psyr_1200 0.78 -1.0 1,359,421 + Psyr_1200 0.78 +2.5 1,359,422 - Psyr_1200 0.78 -0.1 1,359,652 + Psyr_1200 0.89 -0.5 1,359,652 + Psyr_1200 0.89 +0.6 1,359,653 - Psyr_1200 0.89 +0.2 1,359,653 - Psyr_1200 0.89 +1.4 1,359,653 - Psyr_1200 0.89 +0.6 1,359,653 - Psyr_1200 0.89 -0.3 1,359,653 - Psyr_1200 0.89 -0.1 1,359,653 - Psyr_1200 0.89 +0.2 1,359,808 + -0.5 1,359,808 + -0.5 1,359,808 + -0.3 1,359,808 + -1.2 1,359,808 + -0.1 1,359,808 + -1.4 1,359,808 + +1.4 1,359,808 + -1.1 1,359,808 + -0.8 1,359,808 + +0.1 1,359,808 + +0.6 1,359,808 + -0.2 1,359,809 - -0.8 1,359,809 - +2.5 1,359,809 - -1.9 1,359,809 - -0.2 1,359,809 - -0.5 1,359,809 - +0.1 1,359,818 + +0.5 1,359,819 - -0.7 1,359,819 - -0.4 1,360,062 + Psyr_1201 0.87 -0.2 1,360,078 + -0.2 1,360,086 + -0.5 1,360,086 + +1.1 1,360,087 - +2.6 1,360,121 + +0.9 1,360,122 - -0.1 1,360,122 - -1.1 1,360,122 - -1.2 1,360,124 + +2.1 1,360,124 + -0.4 1,360,124 + -0.2 1,360,124 + -1.0 1,360,125 - +1.2 1,360,125 - -0.3 1,360,304 + Psyr_1202 0.61 +1.1 1,360,305 - Psyr_1202 0.61 +0.8 1,360,305 - Psyr_1202 0.61 -0.3 1,360,411 - -1.2 1,360,444 - +0.1 1,360,535 + +0.2 1,360,564 + -3.2 1,360,565 - -1.2 1,360,574 + +0.7 1,360,574 + +0.9 1,360,575 - +0.1 1,360,578 - -0.5 1,360,625 - -2.1 1,360,625 - -0.5 1,360,625 - -0.4 1,360,626 + +2.3 1,360,626 + -2.8 1,360,626 + -0.8 1,360,627 - -0.8 1,360,627 - +0.0 1,360,627 - -1.2 1,360,630 + +0.5 1,360,630 + +0.5 1,360,630 + +0.5 1,360,631 - -0.1 1,360,631 - -0.5 1,360,631 - -0.1 1,360,631 - +1.2 1,360,631 - +0.7 1,360,631 - +0.7 1,360,700 + +1.3 1,360,700 + -2.7 1,360,701 - -0.8 1,360,701 - +0.2 1,360,709 + -0.1 1,360,710 - -1.4 1,360,710 - -0.2 1,360,710 - +3.2 1,360,710 - -0.2 1,360,790 + +0.5 1,360,791 - +2.9 1,360,800 - -0.7 1,360,836 + -0.4 1,360,836 + +0.1 1,360,837 - -1.3 1,360,837 - -0.7 1,360,881 + -1.1 1,360,882 - -0.5 1,360,922 + +0.5 1,360,926 - -0.1 1,360,939 + -0.9 1,360,939 + -0.2 1,360,939 + +0.5 1,360,939 + +0.2 1,360,939 + +0.7 1,360,939 + -0.3 1,360,940 - +1.9 1,360,940 - -0.0 1,360,940 - -0.1 1,360,940 - -1.0 1,360,940 - +0.5 1,360,948 + -0.7 1,360,949 - -0.6 1,360,992 + -0.7 1,360,993 - -0.9 1,360,998 - +0.4 1,360,998 - -0.7 1,361,042 + +1.3 1,361,043 - +0.5 1,361,043 - -0.7 1,361,047 - -0.5 1,361,138 + Psyr_1203 0.11 -0.1 1,361,138 + Psyr_1203 0.11 +1.7 1,361,139 - Psyr_1203 0.11 +1.5 1,361,139 - Psyr_1203 0.11 -1.7 1,361,139 - Psyr_1203 0.11 -0.6 1,361,157 + Psyr_1203 0.13 +1.7 1,361,158 - Psyr_1203 0.14 -2.8 1,361,158 - Psyr_1203 0.14 +0.4 1,361,160 + Psyr_1203 0.14 -0.7 1,361,270 + Psyr_1203 0.27 +0.2 1,361,270 + Psyr_1203 0.27 -0.3 1,361,317 + Psyr_1203 0.32 -0.7 1,361,317 + Psyr_1203 0.32 +0.3 1,361,318 - Psyr_1203 0.32 -1.3 1,361,341 - Psyr_1203 0.35 -0.1 1,361,355 + Psyr_1203 0.36 -1.3 1,361,355 + Psyr_1203 0.36 -0.1 1,361,409 + Psyr_1203 0.43 +0.1 1,361,438 + Psyr_1203 0.46 -1.3 1,361,438 + Psyr_1203 0.46 -2.1 1,361,438 + Psyr_1203 0.46 +0.6 1,361,438 + Psyr_1203 0.46 -0.7 1,361,438 + Psyr_1203 0.46 -0.9
Or see this region's nucleotide sequence