Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0588 and Psyr_0589 are separated by 89 nucleotides Psyr_0589 and Psyr_0590 overlap by 1 nucleotides
Psyr_0588: Psyr_0588 - Transglutaminase-like protein, at 673,124 to 676,402
_0588
Psyr_0589: Psyr_0589 - protein of unknown function DUF403:Protein of unknown function DUF404:Protein of unknown function DUF407, at 676,492 to 678,987
_0589
Psyr_0590: Psyr_0590 - Transglutaminase-like protein, at 678,987 to 679,880
_0590
Position (kb) 676
677
678
679 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 675.570 kb on - strand, within Psyr_0588 at 675.704 kb on + strand, within Psyr_0588 at 675.881 kb on + strand, within Psyr_0588 at 675.974 kb on + strand, within Psyr_0588 at 675.975 kb on - strand, within Psyr_0588 at 676.034 kb on + strand, within Psyr_0588 at 676.035 kb on - strand, within Psyr_0588 at 676.035 kb on - strand, within Psyr_0588 at 676.223 kb on + strand at 676.224 kb on - strand at 676.249 kb on + strand at 676.250 kb on - strand at 676.254 kb on - strand at 676.254 kb on - strand at 676.254 kb on - strand at 676.400 kb on + strand at 676.457 kb on - strand at 676.470 kb on + strand at 676.470 kb on + strand at 676.470 kb on + strand at 676.470 kb on + strand at 676.471 kb on - strand at 676.471 kb on - strand at 676.471 kb on - strand at 676.471 kb on - strand at 676.530 kb on + strand at 676.530 kb on + strand at 676.530 kb on + strand at 676.530 kb on + strand at 676.531 kb on - strand at 676.531 kb on - strand at 676.534 kb on + strand at 676.534 kb on + strand at 676.534 kb on + strand at 676.534 kb on + strand at 676.534 kb on + strand at 676.534 kb on + strand at 676.534 kb on + strand at 676.535 kb on - strand at 676.535 kb on - strand at 676.535 kb on - strand at 676.535 kb on - strand at 676.535 kb on - strand at 676.589 kb on - strand at 676.675 kb on + strand at 676.675 kb on + strand at 676.675 kb on + strand at 676.675 kb on + strand at 676.676 kb on - strand at 676.676 kb on - strand at 676.676 kb on - strand at 676.676 kb on - strand at 676.676 kb on - strand at 676.676 kb on - strand at 676.676 kb on - strand at 676.676 kb on - strand at 676.684 kb on + strand at 676.684 kb on + strand at 676.684 kb on + strand at 676.684 kb on + strand at 676.684 kb on + strand at 676.685 kb on - strand at 676.685 kb on - strand at 676.685 kb on - strand at 676.685 kb on - strand at 676.685 kb on - strand at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.942 kb on + strand, within Psyr_0589 at 676.943 kb on - strand, within Psyr_0589 at 676.943 kb on - strand, within Psyr_0589 at 676.943 kb on - strand, within Psyr_0589 at 676.943 kb on - strand, within Psyr_0589 at 676.943 kb on - strand, within Psyr_0589 at 677.204 kb on - strand, within Psyr_0589 at 677.204 kb on - strand at 677.218 kb on + strand, within Psyr_0589 at 677.219 kb on - strand, within Psyr_0589 at 677.219 kb on - strand, within Psyr_0589 at 677.219 kb on - strand, within Psyr_0589 at 677.287 kb on + strand, within Psyr_0589 at 677.288 kb on - strand, within Psyr_0589 at 677.429 kb on + strand, within Psyr_0589 at 677.429 kb on + strand, within Psyr_0589 at 677.429 kb on + strand, within Psyr_0589 at 677.429 kb on + strand, within Psyr_0589 at 677.429 kb on + strand, within Psyr_0589 at 677.429 kb on + strand, within Psyr_0589 at 677.429 kb on + strand, within Psyr_0589 at 677.429 kb on + strand, within Psyr_0589 at 677.430 kb on - strand, within Psyr_0589 at 677.430 kb on - strand, within Psyr_0589 at 677.430 kb on - strand, within Psyr_0589 at 677.477 kb on + strand, within Psyr_0589 at 677.506 kb on + strand, within Psyr_0589 at 677.711 kb on - strand, within Psyr_0589 at 677.711 kb on - strand, within Psyr_0589 at 677.716 kb on + strand, within Psyr_0589 at 677.716 kb on + strand, within Psyr_0589 at 677.717 kb on - strand, within Psyr_0589 at 677.717 kb on - strand, within Psyr_0589 at 677.717 kb on - strand, within Psyr_0589 at 677.809 kb on + strand, within Psyr_0589 at 677.809 kb on + strand, within Psyr_0589 at 677.810 kb on - strand, within Psyr_0589 at 677.810 kb on - strand, within Psyr_0589 at 677.810 kb on - strand, within Psyr_0589 at 677.810 kb on - strand, within Psyr_0589 at 677.977 kb on - strand, within Psyr_0589 at 677.989 kb on + strand, within Psyr_0589 at 678.013 kb on + strand, within Psyr_0589 at 678.014 kb on - strand, within Psyr_0589 at 678.112 kb on + strand, within Psyr_0589 at 678.113 kb on - strand, within Psyr_0589 at 678.113 kb on - strand, within Psyr_0589 at 678.312 kb on - strand, within Psyr_0589 at 678.619 kb on + strand, within Psyr_0589 at 678.619 kb on + strand, within Psyr_0589 at 678.619 kb on + strand, within Psyr_0589 at 678.631 kb on + strand, within Psyr_0589 at 678.631 kb on + strand, within Psyr_0589 at 678.632 kb on - strand, within Psyr_0589 at 678.632 kb on - strand, within Psyr_0589 at 678.774 kb on + strand at 678.774 kb on + strand at 678.774 kb on + strand at 678.775 kb on - strand at 679.000 kb on - strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.023 kb on + strand at 679.024 kb on - strand at 679.029 kb on + strand at 679.029 kb on + strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.030 kb on - strand at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.308 kb on + strand, within Psyr_0590 at 679.309 kb on - strand, within Psyr_0590 at 679.309 kb on - strand, within Psyr_0590 at 679.447 kb on + strand, within Psyr_0590 at 679.448 kb on - strand, within Psyr_0590 at 679.611 kb on + strand, within Psyr_0590 at 679.612 kb on - strand, within Psyr_0590 at 679.696 kb on + strand, within Psyr_0590 at 679.697 kb on - strand, within Psyr_0590 at 679.878 kb on + strand at 679.878 kb on + strand at 679.882 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 675,570 - Psyr_0588 0.75 -1.4 675,704 + Psyr_0588 0.79 +0.5 675,881 + Psyr_0588 0.84 -0.6 675,974 + Psyr_0588 0.87 -0.3 675,975 - Psyr_0588 0.87 +0.2 676,034 + Psyr_0588 0.89 +0.2 676,035 - Psyr_0588 0.89 +1.1 676,035 - Psyr_0588 0.89 +0.1 676,223 + -1.4 676,224 - +0.9 676,249 + +1.2 676,250 - -0.0 676,254 - -0.0 676,254 - +1.8 676,254 - +0.6 676,400 + -0.4 676,457 - -1.1 676,470 + -1.0 676,470 + +0.8 676,470 + -0.3 676,470 + +1.5 676,471 - +1.6 676,471 - +0.2 676,471 - +1.2 676,471 - -0.6 676,530 + +0.2 676,530 + -1.2 676,530 + -0.1 676,530 + +0.4 676,531 - +0.4 676,531 - -0.5 676,534 + -0.5 676,534 + +0.2 676,534 + -1.6 676,534 + -1.2 676,534 + +1.4 676,534 + +1.0 676,534 + -0.1 676,535 - +2.1 676,535 - +0.9 676,535 - +2.0 676,535 - +0.3 676,535 - +0.3 676,589 - -1.0 676,675 + +0.5 676,675 + -0.9 676,675 + -1.0 676,675 + +2.1 676,676 - -0.3 676,676 - +2.1 676,676 - +0.7 676,676 - -0.5 676,676 - +0.5 676,676 - -0.1 676,676 - +0.0 676,676 - +0.1 676,684 + +0.5 676,684 + +1.3 676,684 + +0.4 676,684 + -0.4 676,684 + +1.2 676,685 - +0.4 676,685 - +0.2 676,685 - +0.6 676,685 - +0.1 676,685 - -0.1 676,942 + Psyr_0589 0.18 -0.0 676,942 + Psyr_0589 0.18 +0.5 676,942 + Psyr_0589 0.18 +1.3 676,942 + Psyr_0589 0.18 +0.1 676,942 + Psyr_0589 0.18 +1.1 676,942 + Psyr_0589 0.18 -0.9 676,942 + Psyr_0589 0.18 -0.6 676,942 + Psyr_0589 0.18 -0.4 676,942 + Psyr_0589 0.18 -0.4 676,942 + Psyr_0589 0.18 -0.9 676,942 + Psyr_0589 0.18 -0.1 676,943 - Psyr_0589 0.18 +0.4 676,943 - Psyr_0589 0.18 -1.6 676,943 - Psyr_0589 0.18 -0.5 676,943 - Psyr_0589 0.18 -1.2 676,943 - Psyr_0589 0.18 +0.7 677,204 - Psyr_0589 0.29 +1.7 677,204 - +2.7 677,218 + Psyr_0589 0.29 -0.3 677,219 - Psyr_0589 0.29 -0.4 677,219 - Psyr_0589 0.29 +0.3 677,219 - Psyr_0589 0.29 -2.2 677,287 + Psyr_0589 0.32 +0.2 677,288 - Psyr_0589 0.32 +0.7 677,429 + Psyr_0589 0.38 +1.1 677,429 + Psyr_0589 0.38 +2.7 677,429 + Psyr_0589 0.38 +0.2 677,429 + Psyr_0589 0.38 +1.2 677,429 + Psyr_0589 0.38 -0.3 677,429 + Psyr_0589 0.38 +1.9 677,429 + Psyr_0589 0.38 -2.1 677,429 + Psyr_0589 0.38 -2.2 677,430 - Psyr_0589 0.38 +1.0 677,430 - Psyr_0589 0.38 -1.8 677,430 - Psyr_0589 0.38 -0.3 677,477 + Psyr_0589 0.39 -0.1 677,506 + Psyr_0589 0.41 +0.4 677,711 - Psyr_0589 0.49 -0.2 677,711 - Psyr_0589 0.49 +0.4 677,716 + Psyr_0589 0.49 -1.0 677,716 + Psyr_0589 0.49 +1.2 677,717 - Psyr_0589 0.49 +0.4 677,717 - Psyr_0589 0.49 +0.6 677,717 - Psyr_0589 0.49 +0.3 677,809 + Psyr_0589 0.53 -0.6 677,809 + Psyr_0589 0.53 -0.2 677,810 - Psyr_0589 0.53 -0.3 677,810 - Psyr_0589 0.53 -0.5 677,810 - Psyr_0589 0.53 -0.0 677,810 - Psyr_0589 0.53 -0.2 677,977 - Psyr_0589 0.59 +0.5 677,989 + Psyr_0589 0.60 -0.5 678,013 + Psyr_0589 0.61 -0.3 678,014 - Psyr_0589 0.61 -1.4 678,112 + Psyr_0589 0.65 +3.4 678,113 - Psyr_0589 0.65 +0.1 678,113 - Psyr_0589 0.65 -0.5 678,312 - Psyr_0589 0.73 +0.5 678,619 + Psyr_0589 0.85 -1.4 678,619 + Psyr_0589 0.85 +0.1 678,619 + Psyr_0589 0.85 +2.1 678,631 + Psyr_0589 0.86 +0.4 678,631 + Psyr_0589 0.86 +0.4 678,632 - Psyr_0589 0.86 -0.2 678,632 - Psyr_0589 0.86 -0.9 678,774 + +0.2 678,774 + -0.2 678,774 + +0.5 678,775 - +0.8 679,000 - +1.4 679,023 + +0.6 679,023 + +0.6 679,023 + +1.1 679,023 + -0.6 679,023 + -1.4 679,023 + -0.4 679,023 + +2.7 679,024 - -3.3 679,029 + -1.8 679,029 + -0.4 679,030 - +0.6 679,030 - -0.8 679,030 - -0.9 679,030 - -0.1 679,030 - +0.3 679,030 - -2.2 679,308 + Psyr_0590 0.36 +0.1 679,308 + Psyr_0590 0.36 -0.0 679,308 + Psyr_0590 0.36 -1.2 679,308 + Psyr_0590 0.36 -0.2 679,308 + Psyr_0590 0.36 -1.7 679,308 + Psyr_0590 0.36 +0.2 679,309 - Psyr_0590 0.36 -0.7 679,309 - Psyr_0590 0.36 +1.6 679,447 + Psyr_0590 0.51 -1.1 679,448 - Psyr_0590 0.52 -1.2 679,611 + Psyr_0590 0.70 -1.0 679,612 - Psyr_0590 0.70 -1.5 679,696 + Psyr_0590 0.79 +0.5 679,697 - Psyr_0590 0.79 -0.9 679,878 + +2.9 679,878 + -0.9 679,882 + -0.5
Or see this region's nucleotide sequence