Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_05104

Experiment: Bu1_10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_05103 and H281DRAFT_05104 are separated by 94 nucleotidesH281DRAFT_05104 and H281DRAFT_05105 are separated by 177 nucleotidesH281DRAFT_05105 and H281DRAFT_05106 are separated by 108 nucleotides H281DRAFT_05103: H281DRAFT_05103 - NusA antitermination factor, at 46,878 to 48,353 _05103 H281DRAFT_05104: H281DRAFT_05104 - bacterial translation initiation factor 2 (bIF-2), at 48,448 to 51,393 _05104 H281DRAFT_05105: H281DRAFT_05105 - ribosome-binding factor A, at 51,571 to 51,936 _05105 H281DRAFT_05106: H281DRAFT_05106 - tRNA pseudouridine synthase B, at 52,045 to 52,980 _05106 Position (kb) 48 49 50 51 52Strain fitness (log2 ratio) -2 -1 0 1at 48.268 kb on + strandat 48.268 kb on + strandat 52.007 kb on + strandat 52.007 kb on + strandat 52.007 kb on + strandat 52.008 kb on - strandat 52.008 kb on - strandat 52.026 kb on + strandat 52.027 kb on - strandat 52.159 kb on + strand, within H281DRAFT_05106at 52.159 kb on + strand, within H281DRAFT_05106at 52.160 kb on - strand, within H281DRAFT_05106at 52.160 kb on - strand, within H281DRAFT_05106at 52.160 kb on - strand, within H281DRAFT_05106at 52.182 kb on + strand, within H281DRAFT_05106at 52.182 kb on + strand, within H281DRAFT_05106at 52.182 kb on + strand, within H281DRAFT_05106at 52.183 kb on - strand, within H281DRAFT_05106

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Per-strain Table

Position Strand Gene LocusTag Fraction Bu1_10
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48,268 + +0.5
48,268 + -0.1
52,007 + -2.0
52,007 + +0.6
52,007 + -1.0
52,008 - -2.5
52,008 - +1.4
52,026 + -0.9
52,027 - -1.2
52,159 + H281DRAFT_05106 0.12 -0.2
52,159 + H281DRAFT_05106 0.12 -1.0
52,160 - H281DRAFT_05106 0.12 -0.0
52,160 - H281DRAFT_05106 0.12 +0.6
52,160 - H281DRAFT_05106 0.12 -0.1
52,182 + H281DRAFT_05106 0.15 -0.5
52,182 + H281DRAFT_05106 0.15 -1.3
52,182 + H281DRAFT_05106 0.15 +1.1
52,183 - H281DRAFT_05106 0.15 -0.5

Or see this region's nucleotide sequence