Experiment: Bu1_10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03009 and H281DRAFT_03010 are separated by 82 nucleotides H281DRAFT_03010 and H281DRAFT_03011 are separated by 63 nucleotides H281DRAFT_03011 and H281DRAFT_03012 are separated by 104 nucleotides H281DRAFT_03012 and H281DRAFT_03013 are separated by 96 nucleotides
H281DRAFT_03009: H281DRAFT_03009 - acetyl coenzyme A synthetase (ADP forming), alpha domain-containing protein, at 35,382 to 37,577
_03009
H281DRAFT_03010: H281DRAFT_03010 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 37,660 to 38,481
_03010
H281DRAFT_03011: H281DRAFT_03011 - NAD(P) transhydrogenase subunit beta, at 38,545 to 39,579
_03011
H281DRAFT_03012: H281DRAFT_03012 - hypothetical protein, at 39,684 to 39,989
_03012
H281DRAFT_03013: H281DRAFT_03013 - transcriptional regulator, LysR family, at 40,086 to 41,033
_03013
Position (kb) 38
39
40 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 37.545 kb on + strand at 37.665 kb on + strand at 37.665 kb on + strand at 37.726 kb on + strand at 37.726 kb on + strand at 37.846 kb on + strand, within H281DRAFT_03010 at 38.334 kb on + strand, within H281DRAFT_03010 at 38.413 kb on - strand at 38.413 kb on - strand at 38.589 kb on - strand at 38.667 kb on - strand, within H281DRAFT_03011 at 39.170 kb on + strand, within H281DRAFT_03011 at 39.171 kb on - strand, within H281DRAFT_03011 at 39.264 kb on - strand, within H281DRAFT_03011 at 39.292 kb on + strand, within H281DRAFT_03011 at 39.472 kb on + strand, within H281DRAFT_03011 at 39.473 kb on - strand, within H281DRAFT_03011 at 39.615 kb on + strand at 39.616 kb on - strand at 39.716 kb on - strand at 39.764 kb on - strand, within H281DRAFT_03012 at 39.764 kb on - strand, within H281DRAFT_03012 at 39.797 kb on + strand, within H281DRAFT_03012 at 39.902 kb on - strand, within H281DRAFT_03012 at 39.902 kb on - strand, within H281DRAFT_03012 at 40.034 kb on + strand at 40.035 kb on - strand at 40.035 kb on - strand at 40.217 kb on - strand, within H281DRAFT_03013 at 40.217 kb on - strand, within H281DRAFT_03013 at 40.292 kb on + strand, within H281DRAFT_03013 at 40.443 kb on + strand, within H281DRAFT_03013 at 40.444 kb on - strand, within H281DRAFT_03013 at 40.509 kb on - strand, within H281DRAFT_03013 at 40.528 kb on + strand, within H281DRAFT_03013
Per-strain Table
Position Strand Gene LocusTag Fraction Bu1_10 remove 37,545 + -2.8 37,665 + +0.6 37,665 + +0.7 37,726 + +0.5 37,726 + +0.5 37,846 + H281DRAFT_03010 0.23 -1.8 38,334 + H281DRAFT_03010 0.82 -0.4 38,413 - +1.5 38,413 - +0.5 38,589 - -3.2 38,667 - H281DRAFT_03011 0.12 +0.3 39,170 + H281DRAFT_03011 0.60 +0.0 39,171 - H281DRAFT_03011 0.60 +0.0 39,264 - H281DRAFT_03011 0.69 -0.4 39,292 + H281DRAFT_03011 0.72 +0.3 39,472 + H281DRAFT_03011 0.90 +0.0 39,473 - H281DRAFT_03011 0.90 -0.7 39,615 + +1.5 39,616 - +0.5 39,716 - +0.3 39,764 - H281DRAFT_03012 0.26 +1.1 39,764 - H281DRAFT_03012 0.26 +0.8 39,797 + H281DRAFT_03012 0.37 -0.5 39,902 - H281DRAFT_03012 0.71 -0.1 39,902 - H281DRAFT_03012 0.71 +0.1 40,034 + -2.3 40,035 - -0.6 40,035 - -0.7 40,217 - H281DRAFT_03013 0.14 -0.1 40,217 - H281DRAFT_03013 0.14 +1.7 40,292 + H281DRAFT_03013 0.22 -1.4 40,443 + H281DRAFT_03013 0.38 +0.3 40,444 - H281DRAFT_03013 0.38 -0.1 40,509 - H281DRAFT_03013 0.45 -0.9 40,528 + H281DRAFT_03013 0.47 -1.4
Or see this region's nucleotide sequence