Experiment: Bu1_10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00296 and H281DRAFT_00297 are separated by 180 nucleotides H281DRAFT_00297 and H281DRAFT_00298 are separated by 195 nucleotides H281DRAFT_00298 and H281DRAFT_00299 are separated by 264 nucleotides
H281DRAFT_00296: H281DRAFT_00296 - poly(3-hydroxybutyrate) depolymerase, at 315,763 to 317,040
_00296
H281DRAFT_00297: H281DRAFT_00297 - Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family, at 317,221 to 319,143
_00297
H281DRAFT_00298: H281DRAFT_00298 - hypothetical protein, at 319,339 to 319,584
_00298
H281DRAFT_00299: H281DRAFT_00299 - Protein of unknown function (DUF3096), at 319,849 to 320,001
_00299
Position (kb)
317
318
319
320 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 316.301 kb on + strand, within H281DRAFT_00296 at 316.346 kb on + strand, within H281DRAFT_00296 at 316.389 kb on + strand, within H281DRAFT_00296 at 316.559 kb on + strand, within H281DRAFT_00296 at 316.625 kb on + strand, within H281DRAFT_00296 at 316.626 kb on - strand, within H281DRAFT_00296 at 316.934 kb on + strand at 316.934 kb on + strand at 317.007 kb on + strand at 317.007 kb on + strand at 317.034 kb on - strand at 317.126 kb on - strand at 317.154 kb on + strand at 317.157 kb on - strand at 317.157 kb on - strand at 317.228 kb on - strand at 317.295 kb on + strand at 317.477 kb on + strand, within H281DRAFT_00297 at 317.478 kb on - strand, within H281DRAFT_00297 at 317.478 kb on - strand, within H281DRAFT_00297 at 317.650 kb on - strand, within H281DRAFT_00297 at 317.656 kb on + strand, within H281DRAFT_00297 at 317.656 kb on + strand, within H281DRAFT_00297 at 317.656 kb on + strand, within H281DRAFT_00297 at 317.657 kb on - strand, within H281DRAFT_00297 at 317.733 kb on - strand, within H281DRAFT_00297 at 317.733 kb on - strand, within H281DRAFT_00297 at 318.062 kb on + strand, within H281DRAFT_00297 at 318.182 kb on + strand, within H281DRAFT_00297 at 318.182 kb on + strand, within H281DRAFT_00297 at 318.182 kb on + strand, within H281DRAFT_00297 at 318.182 kb on + strand, within H281DRAFT_00297 at 318.182 kb on + strand, within H281DRAFT_00297 at 318.183 kb on - strand, within H281DRAFT_00297 at 318.183 kb on - strand, within H281DRAFT_00297 at 318.289 kb on - strand, within H281DRAFT_00297 at 318.320 kb on + strand, within H281DRAFT_00297 at 318.881 kb on - strand, within H281DRAFT_00297 at 319.079 kb on + strand at 319.111 kb on + strand at 319.111 kb on + strand at 319.111 kb on + strand at 319.240 kb on - strand at 319.240 kb on - strand at 319.245 kb on + strand at 319.245 kb on + strand at 319.245 kb on + strand at 319.246 kb on - strand at 319.246 kb on - strand at 319.255 kb on - strand at 319.522 kb on + strand, within H281DRAFT_00298 at 319.523 kb on - strand, within H281DRAFT_00298 at 319.523 kb on - strand, within H281DRAFT_00298 at 319.657 kb on + strand at 319.658 kb on - strand at 319.658 kb on - strand at 319.658 kb on - strand at 319.816 kb on + strand at 319.905 kb on - strand, within H281DRAFT_00299 at 319.917 kb on + strand, within H281DRAFT_00299 at 319.919 kb on + strand, within H281DRAFT_00299 at 319.919 kb on + strand, within H281DRAFT_00299 at 319.919 kb on + strand, within H281DRAFT_00299 at 319.919 kb on + strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.951 kb on + strand, within H281DRAFT_00299 at 319.952 kb on - strand, within H281DRAFT_00299 at 320.005 kb on + strand at 320.006 kb on - strand at 320.012 kb on + strand at 320.024 kb on - strand at 320.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bu1_10 remove 316,301 + H281DRAFT_00296 0.42 -1.7 316,346 + H281DRAFT_00296 0.46 +1.4 316,389 + H281DRAFT_00296 0.49 -2.8 316,559 + H281DRAFT_00296 0.62 +0.6 316,625 + H281DRAFT_00296 0.67 -0.6 316,626 - H281DRAFT_00296 0.68 -2.1 316,934 + +0.3 316,934 + +1.0 317,007 + +0.2 317,007 + +0.3 317,034 - +0.8 317,126 - +0.7 317,154 + +0.2 317,157 - +0.5 317,157 - -1.3 317,228 - +0.1 317,295 + -0.2 317,477 + H281DRAFT_00297 0.13 +1.0 317,478 - H281DRAFT_00297 0.13 +1.9 317,478 - H281DRAFT_00297 0.13 +0.2 317,650 - H281DRAFT_00297 0.22 +0.2 317,656 + H281DRAFT_00297 0.23 -1.7 317,656 + H281DRAFT_00297 0.23 +0.4 317,656 + H281DRAFT_00297 0.23 +0.6 317,657 - H281DRAFT_00297 0.23 -0.1 317,733 - H281DRAFT_00297 0.27 -0.8 317,733 - H281DRAFT_00297 0.27 -2.7 318,062 + H281DRAFT_00297 0.44 +1.2 318,182 + H281DRAFT_00297 0.50 -1.3 318,182 + H281DRAFT_00297 0.50 -0.6 318,182 + H281DRAFT_00297 0.50 +0.6 318,182 + H281DRAFT_00297 0.50 -0.5 318,182 + H281DRAFT_00297 0.50 -0.0 318,183 - H281DRAFT_00297 0.50 +0.5 318,183 - H281DRAFT_00297 0.50 +0.3 318,289 - H281DRAFT_00297 0.56 -1.1 318,320 + H281DRAFT_00297 0.57 +0.6 318,881 - H281DRAFT_00297 0.86 +0.7 319,079 + +0.8 319,111 + +0.5 319,111 + -0.6 319,111 + +0.8 319,240 - -0.3 319,240 - +0.2 319,245 + +0.2 319,245 + -1.3 319,245 + +2.0 319,246 - -0.2 319,246 - -0.4 319,255 - -0.7 319,522 + H281DRAFT_00298 0.74 -0.8 319,523 - H281DRAFT_00298 0.75 -0.7 319,523 - H281DRAFT_00298 0.75 -0.3 319,657 + -1.4 319,658 - -0.4 319,658 - -0.4 319,658 - -1.4 319,816 + +1.0 319,905 - H281DRAFT_00299 0.37 -2.9 319,917 + H281DRAFT_00299 0.44 -1.1 319,919 + H281DRAFT_00299 0.46 +0.3 319,919 + H281DRAFT_00299 0.46 -0.0 319,919 + H281DRAFT_00299 0.46 -2.1 319,919 + H281DRAFT_00299 0.46 -0.1 319,920 - H281DRAFT_00299 0.46 +1.6 319,920 - H281DRAFT_00299 0.46 -0.9 319,920 - H281DRAFT_00299 0.46 -0.1 319,920 - H281DRAFT_00299 0.46 -1.3 319,920 - H281DRAFT_00299 0.46 +0.6 319,920 - H281DRAFT_00299 0.46 -2.7 319,951 + H281DRAFT_00299 0.67 -0.4 319,952 - H281DRAFT_00299 0.67 +0.6 320,005 + -0.9 320,006 - +0.2 320,012 + +0.3 320,024 - -0.6 320,114 - +0.3
Or see this region's nucleotide sequence