Experiment: Carbon source Sucrose 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2575 and Dshi_2576 overlap by 4 nucleotides Dshi_2576 and Dshi_2577 are separated by 100 nucleotides Dshi_2577 and Dshi_2578 are separated by 260 nucleotides
Dshi_2575: Dshi_2575 - phosphoribosyl-ATP pyrophosphohydrolase (RefSeq), at 2,741,315 to 2,741,623
_2575
Dshi_2576: Dshi_2576 - imidazoleglycerol phosphate synthase, cyclase subunit (RefSeq), at 2,741,620 to 2,742,381
_2576
Dshi_2577: Dshi_2577 - putative RNA methylase (RefSeq), at 2,742,482 to 2,743,606
_2577
Dshi_2578: Dshi_2578 - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (RefSeq), at 2,743,867 to 2,744,589
_2578
Position (kb)
2742
2743
2744 Strain fitness (log2 ratio)
-2
-1
0
1 at 2742.564 kb on + strand at 2742.619 kb on - strand, within Dshi_2577 at 2742.704 kb on + strand, within Dshi_2577 at 2742.854 kb on + strand, within Dshi_2577 at 2742.956 kb on - strand, within Dshi_2577 at 2743.133 kb on - strand, within Dshi_2577 at 2743.255 kb on + strand, within Dshi_2577 at 2743.311 kb on + strand, within Dshi_2577 at 2743.373 kb on - strand, within Dshi_2577 at 2743.447 kb on + strand, within Dshi_2577 at 2743.498 kb on + strand at 2743.521 kb on - strand at 2743.629 kb on + strand at 2743.632 kb on - strand at 2743.638 kb on - strand at 2743.690 kb on + strand at 2743.698 kb on - strand at 2743.741 kb on + strand at 2743.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 2.5 mM remove 2,742,564 + -0.9 2,742,619 - Dshi_2577 0.12 -1.5 2,742,704 + Dshi_2577 0.20 +0.4 2,742,854 + Dshi_2577 0.33 -0.1 2,742,956 - Dshi_2577 0.42 -1.7 2,743,133 - Dshi_2577 0.58 -0.6 2,743,255 + Dshi_2577 0.69 -0.3 2,743,311 + Dshi_2577 0.74 +0.3 2,743,373 - Dshi_2577 0.79 -2.1 2,743,447 + Dshi_2577 0.86 -1.1 2,743,498 + -0.4 2,743,521 - -0.7 2,743,629 + -2.5 2,743,632 - +0.4 2,743,638 - -1.2 2,743,690 + +0.5 2,743,698 - +0.9 2,743,741 + +1.4 2,743,855 + -0.2
Or see this region's nucleotide sequence