Experiment: BHIS with Cefoxitin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4339 and BT4340 are separated by 182 nucleotides BT4340 and BT4341 are separated by 428 nucleotides
BT4339: BT4339 - glutamine synthetase (NCBI ptt file), at 5,714,701 to 5,716,890
BT4339
BT4340: BT4340 - hypothetical protein (NCBI ptt file), at 5,717,073 to 5,717,273
BT4340
BT4341: BT4341 - hypothetical protein (NCBI ptt file), at 5,717,702 to 5,719,861
BT4341
Position (kb)
5717
5718 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5716.213 kb on - strand, within BT4339 at 5716.215 kb on - strand, within BT4339 at 5716.322 kb on - strand, within BT4339 at 5716.418 kb on - strand, within BT4339 at 5716.473 kb on + strand, within BT4339 at 5716.631 kb on + strand, within BT4339 at 5716.761 kb on - strand at 5717.064 kb on + strand at 5717.067 kb on - strand at 5717.154 kb on + strand, within BT4340 at 5717.169 kb on - strand, within BT4340 at 5717.170 kb on + strand, within BT4340 at 5717.170 kb on + strand, within BT4340 at 5717.171 kb on - strand, within BT4340 at 5717.211 kb on - strand, within BT4340 at 5717.241 kb on + strand, within BT4340 at 5717.380 kb on + strand at 5717.381 kb on - strand at 5717.425 kb on - strand at 5717.458 kb on + strand at 5717.461 kb on + strand at 5717.462 kb on - strand at 5717.527 kb on + strand at 5717.535 kb on - strand at 5717.668 kb on - strand at 5717.683 kb on + strand at 5717.688 kb on + strand at 5717.702 kb on - strand at 5717.702 kb on - strand at 5717.753 kb on - strand at 5717.761 kb on + strand at 5717.856 kb on + strand at 5717.856 kb on + strand at 5717.856 kb on + strand at 5717.858 kb on + strand at 5717.860 kb on + strand at 5717.863 kb on - strand at 5717.948 kb on + strand, within BT4341 at 5717.966 kb on - strand, within BT4341 at 5717.981 kb on - strand, within BT4341 at 5717.997 kb on - strand, within BT4341 at 5718.071 kb on - strand, within BT4341 at 5718.083 kb on + strand, within BT4341 at 5718.084 kb on - strand, within BT4341 at 5718.155 kb on + strand, within BT4341 at 5718.250 kb on + strand, within BT4341 at 5718.272 kb on - strand, within BT4341
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.0125 mM remove 5,716,213 - BT4339 0.69 +1.0 5,716,215 - BT4339 0.69 +2.7 5,716,322 - BT4339 0.74 -1.0 5,716,418 - BT4339 0.78 +0.0 5,716,473 + BT4339 0.81 -1.0 5,716,631 + BT4339 0.88 -1.0 5,716,761 - -1.6 5,717,064 + +2.7 5,717,067 - -2.0 5,717,154 + BT4340 0.40 -2.2 5,717,169 - BT4340 0.48 -1.3 5,717,170 + BT4340 0.48 +0.0 5,717,170 + BT4340 0.48 -1.6 5,717,171 - BT4340 0.49 -2.0 5,717,211 - BT4340 0.69 -0.7 5,717,241 + BT4340 0.84 -2.3 5,717,380 + -2.0 5,717,381 - +0.0 5,717,425 - -3.8 5,717,458 + +2.0 5,717,461 + -1.6 5,717,462 - -3.0 5,717,527 + -1.6 5,717,535 - +0.5 5,717,668 - -1.3 5,717,683 + -0.6 5,717,688 + -1.7 5,717,702 - -2.1 5,717,702 - -0.2 5,717,753 - -1.3 5,717,761 + +0.7 5,717,856 + -3.0 5,717,856 + -2.8 5,717,856 + +2.0 5,717,858 + -0.7 5,717,860 + -1.5 5,717,863 - -2.3 5,717,948 + BT4341 0.11 +0.0 5,717,966 - BT4341 0.12 -1.1 5,717,981 - BT4341 0.13 +0.0 5,717,997 - BT4341 0.14 -1.0 5,718,071 - BT4341 0.17 -3.8 5,718,083 + BT4341 0.18 +1.2 5,718,084 - BT4341 0.18 -3.9 5,718,155 + BT4341 0.21 -1.6 5,718,250 + BT4341 0.25 -1.6 5,718,272 - BT4341 0.26 +0.6
Or see this region's nucleotide sequence