Experiment: BHIS with Cefoxitin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1943 and BT1944 are separated by 14 nucleotides BT1944 and BT1945 overlap by 79 nucleotides BT1945 and BT1946 are separated by 92 nucleotides BT1946 and BT1947 are separated by 29 nucleotides
BT1943: BT1943 - hypothetical protein (NCBI ptt file), at 2,445,288 to 2,445,551
BT1943
BT1944: BT1944 - putative transposase (NCBI ptt file), at 2,445,566 to 2,445,970
BT1944
BT1945: BT1945 - conserved protein found in conjugate transposon (NCBI ptt file), at 2,445,892 to 2,446,674
BT1945
BT1946: BT1946 - hypothetical protein (NCBI ptt file), at 2,446,767 to 2,447,153
BT1946
BT1947: BT1947 - hypothetical protein (NCBI ptt file), at 2,447,183 to 2,447,599
BT1947
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2444.956 kb on - strand at 2444.992 kb on - strand at 2445.009 kb on - strand at 2445.047 kb on - strand at 2445.080 kb on - strand at 2445.136 kb on + strand at 2445.137 kb on - strand at 2445.180 kb on + strand at 2445.188 kb on + strand at 2445.218 kb on - strand at 2445.346 kb on - strand, within BT1943 at 2445.351 kb on + strand, within BT1943 at 2445.366 kb on - strand, within BT1943 at 2445.470 kb on + strand, within BT1943 at 2445.470 kb on + strand, within BT1943 at 2445.470 kb on + strand, within BT1943 at 2445.470 kb on + strand, within BT1943 at 2445.531 kb on - strand at 2445.632 kb on + strand, within BT1944 at 2445.632 kb on + strand, within BT1944 at 2445.642 kb on + strand, within BT1944 at 2445.643 kb on - strand, within BT1944 at 2445.738 kb on - strand, within BT1944 at 2445.785 kb on + strand, within BT1944 at 2445.793 kb on + strand, within BT1944 at 2445.842 kb on - strand, within BT1944 at 2445.894 kb on - strand at 2446.012 kb on - strand, within BT1945 at 2446.113 kb on - strand at 2446.134 kb on + strand, within BT1945 at 2446.215 kb on + strand, within BT1945 at 2446.243 kb on - strand, within BT1945 at 2446.269 kb on + strand, within BT1945 at 2446.270 kb on - strand, within BT1945 at 2446.271 kb on + strand, within BT1945 at 2446.368 kb on + strand, within BT1945 at 2446.371 kb on - strand, within BT1945 at 2446.371 kb on - strand, within BT1945 at 2446.602 kb on + strand at 2446.602 kb on + strand at 2446.696 kb on - strand at 2446.819 kb on + strand, within BT1946 at 2446.819 kb on + strand, within BT1946 at 2447.028 kb on - strand, within BT1946 at 2447.156 kb on + strand at 2447.431 kb on - strand, within BT1947 at 2447.483 kb on + strand, within BT1947
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.0125 mM remove 2,444,956 - -2.0 2,444,992 - +0.8 2,445,009 - -0.0 2,445,047 - +0.4 2,445,080 - -1.8 2,445,136 + -1.4 2,445,137 - -0.6 2,445,180 + -1.1 2,445,188 + -1.3 2,445,218 - -0.7 2,445,346 - BT1943 0.22 -2.6 2,445,351 + BT1943 0.24 -1.6 2,445,366 - BT1943 0.30 -1.1 2,445,470 + BT1943 0.69 -2.6 2,445,470 + BT1943 0.69 -1.6 2,445,470 + BT1943 0.69 -1.3 2,445,470 + BT1943 0.69 -3.3 2,445,531 - -1.0 2,445,632 + BT1944 0.16 +1.0 2,445,632 + BT1944 0.16 -1.9 2,445,642 + BT1944 0.19 -2.6 2,445,643 - BT1944 0.19 -0.0 2,445,738 - BT1944 0.42 -0.0 2,445,785 + BT1944 0.54 -0.0 2,445,793 + BT1944 0.56 +1.1 2,445,842 - BT1944 0.68 -0.0 2,445,894 - +1.0 2,446,012 - BT1945 0.15 -1.6 2,446,113 - -0.0 2,446,134 + BT1945 0.31 -3.3 2,446,215 + BT1945 0.41 -1.6 2,446,243 - BT1945 0.45 -1.6 2,446,269 + BT1945 0.48 -1.7 2,446,270 - BT1945 0.48 -2.3 2,446,271 + BT1945 0.48 -1.0 2,446,368 + BT1945 0.61 -0.9 2,446,371 - BT1945 0.61 -4.1 2,446,371 - BT1945 0.61 -1.6 2,446,602 + +0.3 2,446,602 + -0.2 2,446,696 - -0.3 2,446,819 + BT1946 0.13 -1.9 2,446,819 + BT1946 0.13 -1.5 2,447,028 - BT1946 0.67 -1.0 2,447,156 + -1.0 2,447,431 - BT1947 0.59 -0.8 2,447,483 + BT1947 0.72 -1.6
Or see this region's nucleotide sequence