Experiment: BHIS with Cefoxitin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0929 and pseudo-tRNA are separated by 357 nucleotides pseudo-tRNA and BT0930 are separated by 113 nucleotides BT0930 and BT0931 are separated by 55 nucleotides
BT0929: BT0929 - prolyl-tRNA synthetase (NCBI ptt file), at 1,142,831 to 1,144,324
BT0929
BT_t17: pseudo-tRNA - tRNA-OTHER (RefSeq), at 1,144,682 to 1,144,760
pseudo-tRNA
BT0930: BT0930 - hypothetical protein (NCBI ptt file), at 1,144,874 to 1,145,074
BT0930
BT0931: BT0931 - hypothetical protein (NCBI ptt file), at 1,145,130 to 1,145,774
BT0931
Position (kb)
1144
1145
1146 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1144.430 kb on + strand at 1144.431 kb on - strand at 1144.460 kb on + strand at 1144.463 kb on + strand at 1144.464 kb on - strand at 1144.465 kb on + strand at 1144.494 kb on + strand at 1144.497 kb on - strand at 1144.542 kb on - strand at 1144.544 kb on + strand at 1144.588 kb on + strand at 1144.605 kb on - strand at 1144.717 kb on - strand, within pseudo-tRNA at 1144.730 kb on - strand, within pseudo-tRNA at 1144.730 kb on - strand, within pseudo-tRNA at 1144.783 kb on - strand at 1144.798 kb on - strand at 1144.807 kb on + strand at 1144.826 kb on - strand at 1144.888 kb on - strand at 1144.940 kb on - strand, within BT0930 at 1144.944 kb on - strand, within BT0930 at 1144.946 kb on - strand, within BT0930 at 1144.969 kb on - strand, within BT0930 at 1144.969 kb on - strand, within BT0930 at 1144.990 kb on - strand, within BT0930 at 1144.992 kb on - strand, within BT0930 at 1145.047 kb on - strand, within BT0930 at 1145.088 kb on - strand at 1145.102 kb on - strand at 1145.103 kb on + strand at 1145.127 kb on + strand at 1145.166 kb on - strand at 1145.220 kb on - strand, within BT0931 at 1145.222 kb on - strand, within BT0931 at 1145.229 kb on - strand, within BT0931 at 1145.284 kb on - strand, within BT0931 at 1145.306 kb on - strand, within BT0931 at 1145.316 kb on - strand, within BT0931 at 1145.351 kb on + strand, within BT0931 at 1145.390 kb on + strand, within BT0931 at 1145.424 kb on + strand, within BT0931 at 1145.506 kb on + strand, within BT0931 at 1145.510 kb on + strand, within BT0931 at 1145.511 kb on - strand, within BT0931 at 1145.543 kb on - strand, within BT0931 at 1145.583 kb on - strand, within BT0931 at 1145.648 kb on + strand, within BT0931 at 1145.649 kb on - strand, within BT0931 at 1145.670 kb on - strand, within BT0931 at 1145.670 kb on - strand, within BT0931 at 1145.805 kb on - strand at 1145.871 kb on + strand at 1145.990 kb on - strand at 1146.038 kb on - strand at 1146.052 kb on - strand at 1146.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.0125 mM remove 1,144,430 + -1.1 1,144,431 - -0.1 1,144,460 + -1.1 1,144,463 + -1.3 1,144,464 - -2.1 1,144,465 + -0.1 1,144,494 + -0.7 1,144,497 - -1.4 1,144,542 - +0.1 1,144,544 + -0.8 1,144,588 + -1.1 1,144,605 - -1.1 1,144,717 - pseudo-tRNA BT_t17 0.44 -1.9 1,144,730 - pseudo-tRNA BT_t17 0.61 -1.0 1,144,730 - pseudo-tRNA BT_t17 0.61 -1.4 1,144,783 - -2.4 1,144,798 - -1.2 1,144,807 + -2.1 1,144,826 - -0.1 1,144,888 - -2.1 1,144,940 - BT0930 0.33 -0.1 1,144,944 - BT0930 0.35 -3.4 1,144,946 - BT0930 0.36 -2.7 1,144,969 - BT0930 0.47 -3.2 1,144,969 - BT0930 0.47 -0.1 1,144,990 - BT0930 0.58 -1.7 1,144,992 - BT0930 0.59 -1.9 1,145,047 - BT0930 0.86 -3.1 1,145,088 - -1.7 1,145,102 - -0.1 1,145,103 + -2.4 1,145,127 + +2.9 1,145,166 - -1.1 1,145,220 - BT0931 0.14 -0.1 1,145,222 - BT0931 0.14 -1.7 1,145,229 - BT0931 0.15 -2.9 1,145,284 - BT0931 0.24 -2.1 1,145,306 - BT0931 0.27 -0.1 1,145,316 - BT0931 0.29 -0.1 1,145,351 + BT0931 0.34 +2.5 1,145,390 + BT0931 0.40 -0.7 1,145,424 + BT0931 0.46 -1.1 1,145,506 + BT0931 0.58 -0.1 1,145,510 + BT0931 0.59 -1.7 1,145,511 - BT0931 0.59 +1.5 1,145,543 - BT0931 0.64 -1.9 1,145,583 - BT0931 0.70 +0.9 1,145,648 + BT0931 0.80 -2.7 1,145,649 - BT0931 0.80 -2.2 1,145,670 - BT0931 0.84 -1.4 1,145,670 - BT0931 0.84 +0.3 1,145,805 - +0.4 1,145,871 + -0.1 1,145,990 - -3.1 1,146,038 - -1.2 1,146,052 - -0.5 1,146,062 - -2.4
Or see this region's nucleotide sequence