Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0010

Experiment: BHIS with Cefoxitin 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0009 and BT0010 are separated by 54 nucleotidesBT0010 and BT0011 are separated by 205 nucleotides BT0009: BT0009 - phosphate-binding periplasmic protein precursor, putative ABC transporter (NCBI ptt file), at 5,632 to 6,741 BT0009 BT0010: BT0010 - hypothetical protein (NCBI ptt file), at 6,796 to 7,869 BT0010 BT0011: BT0011 - hypothetical protein (NCBI ptt file), at 8,075 to 8,923 BT0011 Position (kb) 6 7 8Strain fitness (log2 ratio) -2 -1 0 1 2at 5.868 kb on + strand, within BT0009at 5.925 kb on - strand, within BT0009at 5.925 kb on - strand, within BT0009at 5.989 kb on - strand, within BT0009at 6.001 kb on - strand, within BT0009at 6.086 kb on - strand, within BT0009at 6.086 kb on - strand, within BT0009at 6.086 kb on - strand, within BT0009at 6.218 kb on - strand, within BT0009at 6.516 kb on + strand, within BT0009at 6.540 kb on - strand, within BT0009at 6.545 kb on - strand, within BT0009at 6.546 kb on + strand, within BT0009at 6.716 kb on - strandat 6.716 kb on - strandat 6.744 kb on + strandat 6.750 kb on + strandat 6.751 kb on - strandat 6.758 kb on + strandat 6.759 kb on - strandat 6.834 kb on + strandat 6.858 kb on - strandat 6.858 kb on - strandat 6.925 kb on - strand, within BT0010at 7.277 kb on + strand, within BT0010at 7.277 kb on + strand, within BT0010at 7.348 kb on - strand, within BT0010at 7.348 kb on - strand, within BT0010at 7.353 kb on - strand, within BT0010at 7.614 kb on - strand, within BT0010at 7.627 kb on - strand, within BT0010at 7.756 kb on + strand, within BT0010at 7.908 kb on + strandat 7.909 kb on - strandat 7.937 kb on - strandat 7.967 kb on - strandat 7.996 kb on + strandat 7.997 kb on - strandat 8.059 kb on - strandat 8.097 kb on - strandat 8.138 kb on - strandat 8.138 kb on - strandat 8.154 kb on - strandat 8.393 kb on + strand, within BT0011at 8.393 kb on + strand, within BT0011at 8.394 kb on - strand, within BT0011at 8.448 kb on - strand, within BT0011at 8.448 kb on - strand, within BT0011at 8.472 kb on - strand, within BT0011at 8.497 kb on - strand, within BT0011at 8.505 kb on - strand, within BT0011at 8.505 kb on - strand, within BT0011at 8.550 kb on - strand, within BT0011at 8.550 kb on - strand, within BT0011at 8.550 kb on - strand, within BT0011at 8.551 kb on + strand, within BT0011at 8.681 kb on + strand, within BT0011at 8.688 kb on - strand, within BT0011at 8.711 kb on - strand, within BT0011at 8.767 kb on + strand, within BT0011

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Cefoxitin 0.0125 mM
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5,868 + BT0009 0.21 -2.1
5,925 - BT0009 0.26 -1.7
5,925 - BT0009 0.26 -1.0
5,989 - BT0009 0.32 -2.0
6,001 - BT0009 0.33 -1.9
6,086 - BT0009 0.41 +2.0
6,086 - BT0009 0.41 +0.2
6,086 - BT0009 0.41 +0.0
6,218 - BT0009 0.53 -1.1
6,516 + BT0009 0.80 -2.3
6,540 - BT0009 0.82 -1.5
6,545 - BT0009 0.82 -1.8
6,546 + BT0009 0.82 -0.4
6,716 - -0.8
6,716 - +0.6
6,744 + +2.3
6,750 + -2.3
6,751 - +1.6
6,758 + -2.4
6,759 - -1.5
6,834 + +1.0
6,858 - +1.6
6,858 - -0.2
6,925 - BT0010 0.12 +0.0
7,277 + BT0010 0.45 +0.6
7,277 + BT0010 0.45 +2.0
7,348 - BT0010 0.51 -1.0
7,348 - BT0010 0.51 +0.4
7,353 - BT0010 0.52 -1.6
7,614 - BT0010 0.76 +1.0
7,627 - BT0010 0.77 -1.6
7,756 + BT0010 0.89 +0.0
7,908 + -2.3
7,909 - -1.0
7,937 - +1.6
7,967 - -1.9
7,996 + -2.0
7,997 - -2.6
8,059 - +0.0
8,097 - -1.9
8,138 - +0.3
8,138 - -1.0
8,154 - -1.1
8,393 + BT0011 0.37 -2.4
8,393 + BT0011 0.37 -1.0
8,394 - BT0011 0.38 -2.0
8,448 - BT0011 0.44 -1.6
8,448 - BT0011 0.44 -1.6
8,472 - BT0011 0.47 -2.3
8,497 - BT0011 0.50 -2.0
8,505 - BT0011 0.51 +0.4
8,505 - BT0011 0.51 -1.0
8,550 - BT0011 0.56 -1.3
8,550 - BT0011 0.56 -2.2
8,550 - BT0011 0.56 +0.0
8,551 + BT0011 0.56 +0.0
8,681 + BT0011 0.71 -0.4
8,688 - BT0011 0.72 +1.2
8,711 - BT0011 0.75 -0.4
8,767 + BT0011 0.82 -1.0

Or see this region's nucleotide sequence